Live single cell imaging assays in glass microwells produced by laser-induced deep etching

Abstract

Miniaturization of cell culture substrates enables controlled analysis of living cells in confined micro-scale environments. This is particularly suitable for imaging individual cells over time, as they can be precisely...

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

“…However, glass microfabrication is limited in terms of the freedom of design, quality, or throughput. Laser-induced deep etching (LIDE) can serve as a method for producing glass microwell arrays for live single-cell imaging assays . LIDE consists of an initial nonablating, laser exposure of glass followed by wet chemical etching and produces defect-free through holes with a high aspect ratio (Figure A).…”

“…Reproduced from Sandström, N.; Brandt, L.; Sandoz, P. A.; Zambarda, C.; Guldevall, K.; Schulz-Ruhtenberg, M.; Rösener, B.; Krüger, R. A.; Önfelt, B. Lab Chip 2022 , 22 , 2107–2121 (ref ), with permission of The Royal Society of Chemistry. (B) Light-scattering control in tomographic volumetric printing enables 3D printing in cell-laden hydrogels (ref ).…”

“…Laser-induced deep etching (LIDE) can serve as a method for producing glass microwell arrays for live single-cell imaging assays. 1 LIDE consists of an initial nonablating, laser exposure of glass followed by wet chemical etching and produces defect-free through holes with a high aspect ratio (Figure 1A). Glass plates with 3D-shaped bottom microwell designs were fabricated using LIDE and used for cell imaging assays with single-cell analysis.…”