Liver cirrhosis in rats: Regeneration and assessment of the role of phenobarbital

Rózga, Jacek; Foss, A.; Alumets, J.; Åhrén, Bo; Jeppsson, Bengt; Bengmark, S

doi:10.1016/0022-4804(91)90116-4

Cited by 19 publications

(28 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The rats treated with phenobarbital alone had loss of a regenerative capacity greater than that of the cirrhotic rats [10]. Therefore, phenobarbital either stimulates or inhibits the regenerative response following partial hepatectomy.…”

Section: Discussionmentioning

confidence: 97%

“…If body weight increased, the dose was increased by 0.04 ml/week. However, if body weight decreased, the administration of CCl 4 was decreased or discontinued for 1 week, depending on the loss in body weight [7,10]. The administration of CCl 4 continued for 10 weeks, and phenobarbital was continued throughout the CCl 4 feeding.…”

Section: Establishment Of Phenobarbital Rats and Cirrhotic Rats Malementioning

confidence: 99%

See 1 more Smart Citation

Phenobarbital in Comparison with Carbon Tetrachloride and Phenobarbital-Induced Cirrhosis in Rat Liver Regeneration

Hashimoto

Kothary

Raper

1999

Journal of Surgical Research

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 97%

Section: Establishment Of Phenobarbital Rats and Cirrhotic Rats Malementioning

confidence: 99%

Phenobarbital in Comparison with Carbon Tetrachloride and Phenobarbital-Induced Cirrhosis in Rat Liver Regeneration

Hashimoto

Kothary

Raper

1999

Journal of Surgical Research

View full text Add to dashboard Cite

“…Necrosis occurs predominantly in the pericentral region of the liver lobule. Regeneration in the CCl 4 -induced rat model was associated with delayed DNA synthesis and mitotic activity in the rat compared to the partial hepatectomy (PHx) model as reported by Rozga et al [10]. …”

Section: Toxin Injury Modelsmentioning

confidence: 81%

Rodent Models and Imaging Techniques to Study Liver Regeneration

Wei

Dirsch²,

McLean³

et al. 2014

Eur Surg Res

View full text Add to dashboard Cite

The liver has the unique capability of regeneration from various injuries. Different animal models and in vitro methods are used for studying the processes and mechanisms of liver regeneration. Animal models were established either by administration of hepatotoxic chemicals or by surgical approach. The administration of hepatotoxic chemicals results in the death of liver cells and in subsequent hepatic regeneration and tissue repair. Surgery includes partial hepatectomy and portal vein occlusion or diversion: hepatectomy leads to compensatory regeneration of the remnant liver lobe, whereas portal vein occlusion leads to atrophy of the ipsilateral lobe and to compensatory regeneration of the contralateral lobe. Adaptation of modern radiological imaging technologies to the small size of rodents made the visualization of rodent intrahepatic vascular anatomy possible. Advanced knowledge of the detailed intrahepatic 3D anatomy enabled the establishment of refined surgical techniques. The same technology allows the visualization of hepatic vascular regeneration. The development of modern histological image analysis tools improved the quantitative assessment of hepatic regeneration. Novel image analysis tools enable us to quantify reliably and reproducibly the proliferative rate of hepatocytes using whole-slide scans, thus reducing the sampling error. In this review, the refined rodent models and the newly developed imaging technology to study liver regeneration are summarized. This summary helps to integrate the current knowledge of liver regeneration and promises an enormous increase in hepatological knowledge in the near future. © 2014 S. Karger AG, Basel

show abstract

“…This repetitive hepatic destruction and regeneration leads to irreversible micronodular cirrhosis in rats in at least 4 months. Simultaneous administration of phenobarbital acts synergistically to faster cirrhosis establishment because it induces hepatic enzymes, increases the activity of cytochrome P 450 and finally potentiates the sensitivity of hepatocytes to CCl 4 [23] . Phenobarbital was added in the drinking water of the animals 14 days before the initial administration of CCl 4 , whereas its continuous administration till the end of the experiment seems to be necessary for faster cirrhosis establishment [24,25] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Experimentally Induced Liver Cirrhosis on Wound Healing of the Post-Extraction Tooth Socket in Rats

Karalis¹,

Pavlidis²,

Psarras³

et al. 2007

Eur Surg Res

View full text Add to dashboard Cite

Background: Wound healing in liver cirrhosis is known to be impaired possibly due to liver insufficiency and subsequent malnutrition status; however, there is no study to examine healing effectiveness of the tooth socket following an extraction in such patients. Materials and Methods: Irreversible cirrhosiswas induced in 30 Wistar rats by repetitive weekly doses of CCl₄ and continuous administration of phenobarbital in a 12-week course was monitored by body weight measurement and ascites development, and was proved histologically. One week later, cirrhotic and control rats were subjected to extractions of two maxillary grinders on each side, one side by simple method, the other by surgical method. Half of the animals of each subgroup were sacrificed on the 10th post-extraction day, whereas the other half on the 30th post-extraction day, and histological sections were examined from all tooth sockets for wound-healing activity. Results: A malnutrition status was detected in cirrhotic rats with significant difference in their body weight. Several histological parameters of socket healing were not statistically different between cirrhotic and control animals. However, a significant delay on epithelialization and cancellous bone formation was detected on the 10th post-extraction day for either simple or surgical extractions in cirrhotic animals. Conclusions: Liver cirrhosis in rats provokes a significant delay on epithelialization and mature cancellous bone formation and consecutively on early socket wound healing after a tooth extraction.

show abstract

Liver cirrhosis in rats: Regeneration and assessment of the role of phenobarbital

Cited by 19 publications

References 17 publications

Phenobarbital in Comparison with Carbon Tetrachloride and Phenobarbital-Induced Cirrhosis in Rat Liver Regeneration

Phenobarbital in Comparison with Carbon Tetrachloride and Phenobarbital-Induced Cirrhosis in Rat Liver Regeneration

Rodent Models and Imaging Techniques to Study Liver Regeneration

Effect of Experimentally Induced Liver Cirrhosis on Wound Healing of the Post-Extraction Tooth Socket in Rats

Contact Info

Product

Resources

About