Liver metastasis models of human colorectal carcinoma established in nude mice by orthotopic transplantation and their biologic characteristic

Liu, Qiu-Zhen; Tuo, Chao-wei; Wang, Bin; Wu, Bingquan; Zhang, Yanhua

doi:10.3748/wjg.v4.i5.409

Cited by 6 publications

(2 citation statements)

References 3 publications

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“…The site of transplantation is usually between the serous layer and seromuscular layer of the greater curvature of stomach. The "suture method" [20] or the "gastric bursa method" [21][22] has many practical difficulties and disadvantag es in establishing the model of transplantation, for example, the tumor tissue is easy to fall off postoperatively; manipulation is complex and needs relatively high skills; and there may be great blood loss during suturing, and mortality is relatively high. OB glue is biologic glue and has been used widely in surgery owing to its secure wound adhesion.…”

Section: Discussionmentioning

confidence: 99%

OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models

Shi¹,

Wei²,

Shen³

et al. 2008

WJG

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AIM:To establish nude mouse human gastric cancer orthotopic transplantation models using OB glue paste technique. METHODS: Using OB glue paste technique, orthotopic transplantation models were established by implanting SGC-7901 and MKN-45 human gastric cancer cell strains into the gastric wall of nude mice. Biological features, growth of the implanted tumors, the success rate of transplantation and the rate of auto-metastasis of the two models were observed. RESULTS: The success rates of orthotopic transplantation of the two models were 94.20% and 96%. The rates of hepatic metastasis, pulmonary metastasis, peritoneal metastasis, lymphocytic metastasis and splenic metastasis were 42.13% and 94.20%, 48.43% and 57.97%, 30.83% and 36.96%, 67.30% and 84.06%, and 59.75% and 10.53%, respectively. The occurrence of ascites was 47.80% and 36.96%. CONCLUSION: OB glue paste technique is easy to follow. The biological behaviors of the nude mouse human gastric cancer orthotopic transplantation models established with this technique are similar to the natural processes of growth and metastasis of human gastric cancer, and, therefore, can be used as an ideal model for experimental research of proliferative metastasis of tumors.

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Section: Discussionmentioning

confidence: 99%

OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models

Shi¹,

Wei²,

Shen³

et al. 2008

WJG

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show abstract

“…With the combination of TRAIL and chemotherapy, the dose of chemotherapeutic agent could be reduced, and thus decreased the side effects of chemotherapy. In order to explore a more effective approach to deliver TRAIL gene for colorectal treatment, or to evaluate the effect of TRAIL gene on liver metastasis (through liver metastasis models of human colorectal carcinoma established in nude mice) [31] ,further research on it is necessary.…”

Section: Discussionmentioning

confidence: 99%

Tumor necrosis factor-related apoptosis-inducing ligand gene on human colorectal cancer cell line HT29

et al. 2003

WJG

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AIM:To evaluate the therapeutic efficiency of Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL) gene on human colorectal cancer cell line HT29. METHODS:Human embryonal kidney cells transformed by introducing sheared fragments of Ad5 DNA (293 cell) were used for amplification of adenoviral vectors: Ad/GT-TRAIL,Ad/GT-Bax, Ad/GT-LacZ and Ad/PGK-GV16. Human colorectal cancer cell line HT29 was transfected with binary adenovirus-mediated TRAIL gene. Bax gene was used as positive control, LacZ gene was used as the vector control, and cells treated with PBS only were used as a mock control. The morphological changes, cell growth and apoptosis were measured by reversmicroscope, MTT method and flow cytometry. RESULTS:All adenoviral vectors titer determined by optical absorbency at A260nm were 1×10 10 viral particle/ml(vp/ ml).Obviously morphological changes of HT29 cells were observed when infected with Ad/GT-TRAIL, and these changes were much more obviously when Ad/PGK-GV16 was coinfected. The cell suppression percentage and the percentage of apoptotic cells were 52.5 % and 16.5 % respectively when infected with Ad/GT-TRAIL alone, while combining with Ad/PGK-GV16, the growth of HT29 was suppressed by 85.2 % and the percentage of apoptotic cells was 35.9 %. It showed a significantly enhanced therapeutic efficiency with binary system (P<0.05). CONCLUSION:A binary adenoviral vector system provides an effective approach to amplify viral vectors that express potentially toxic gene, TRAIL. Ad/GT-TRAIL showed a significantly enhanced therapeutic efficiency for HT29 when coinfected with Ad/PGK-GV16. Ad/GT-TRAIL could induce apoptosis of HT29 and inhibit its growth.

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Activity of boanmycin against colorectal cancer

Deng

Yong²,

Zheng³

et al. 2001

WJG

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Liver metastasis models of human colorectal carcinoma established in nude mice by orthotopic transplantation and their biologic characteristic

Cited by 6 publications

References 3 publications

OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models

OB glue paste technique for establishing nude mouse human gastric cancer orthotopic transplantation models

Tumor necrosis factor-related apoptosis-inducing ligand gene on human colorectal cancer cell line HT29

Activity of boanmycin against colorectal cancer

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