Liver Proteome of Mice with Distinct Genetic Susceptibilities to Fluorosis Treated with Different Concentrations of F in the Drinking Water

Abstract

Appropriate doses of fluoride (F) have therapeutic action against dental caries, but higher levels can cause disturbances in soft and mineralized tissues. Interestingly, the susceptibility to the toxic effects of F is genetically determined. This study evaluated the effects of F on the liver proteome of mice susceptible (A/J) or resistant (129P3/J) to the effects of F. Weanling male A/J (n = 12) and 129P3/J (n = 12) mice were housed in pairs and assigned to two groups given low-F food and drinking water containing 15 or 50 ppm F for 6 weeks. Liver proteome profiles were examined using nano-LC-ESI-MS/MS. Difference in expression among the groups was determined using the PLGS software. Treatment with the lower F concentration provoked more pronounced alterations in fold change in liver proteins in comparison to the treatment with the higher F concentration. Interestingly, most of the proteins with fold change upon treatment with 15 ppm F were increased in the A/J mice compared with their 129P3/J counterparts, suggesting an attempt of the former to fight the deleterious effects of F. However, upon treatment with 50 ppm F, most proteins with fold change were decreased in the A/J mice compared with their 129P3/J counterparts, especially proteins related to oxidative stress and protein folding, which might be related to the higher susceptibility of the A/J animals to the deleterious effects of F. Our findings add light into the mechanisms underlying genetic susceptibility to fluorosis.

Moringa Oleifera Leaf Extract Repairs the Oxidative Misbalance following Sub-Chronic Exposure to Sodium Fluoride in Nile Tilapia Oreochromis niloticus

“…These findings may suggest the economic and medicinal needs to find suitable herbal remedy to reduce these adverse effects of fluoride on fish. The exposure to high concentrations of fluoride could trigger the adverse toxic impact on different biological processes [8]. In different words, low concentration of fluoride (µM) might act as a promoter for cell proliferation and enzymatic activity; however, a high concentration of fluoride (mM) could act as an enzymatic inhibitor.…”

“…Few previous studies concluded the role of fluoride as a toxic, cumulative, environmental pollutant in aquatic life [8] where they are regularly exposed to elevated concentrations of fluoride in surface waters. The adverse impact of fluoride was ensured when it enters the food chain and accumulates in different tissues of aquatic organisms, particularly fish bone and the invertebrate's exoskeleton.…”

Chronic Exposure to Sodium Fluoride Triggers Oxidative Biochemistry Misbalance in Mice: Effects on Peripheral Blood Circulation

“…Small fluoride concentrations have therapeutical action against dental caries. However, exposure to high doses from water ingestion and the use of fluoride toothpastes or fluoride-rich diets increases the body burden of this ion [ 2 ].…”

Proteomic analysis of serum samples of paracoccidioidomycosis patients with severe pulmonary sequel

“…After depletion, Bradford assays [ 39 ] were performed to quantify proteins present in the pooled samples (n = 3 / group) and all samples were standardized to a concentration of 1μg/μL. Samples were submitted to proteomic analysis as previously described [ 40 ]. To 50 μL sample, 10 μL of 50 mM ammonium bicarbonate was added, before the following steps.…”