Liver Regeneration Following Radiofrequency Ablation

Mesna Preserves Hepatocyte Regenerating Capacity Following Liver Radiofrequency Ablation Under Pringle Maneuver

“…Nevertheless, mesna prophylactic administration minimized tissue injury in the non-ablated liver parenchyma that undertakes functional liver volume restoration. Accumulating experimental data have demonstrated the protective effect of mesna against I/ R injury in the liver [7,13,14], as well as other splanchnic organs [9,[14][15][16]. In agreement with previous studies, the protective effect of mesna was associated with inhibition of NF-kB activation.…”

“…In a previous experimental study, it has been reported that RFA induces liver regeneration [7]. As confirmed by serial CT scans, liver parenchyma was fully restored within a 10 wk period after RFA on the 15%-20% of liver mass in the rabbit.…”

“…We have previously shown that RFA on 15%-20% of total liver volume induces slow but sustained hepatocyte mitotic activity in the rabbit [7]. While liver ischemia/reperfusion (I/R) has been proven to be an effective stimulant of hepatocyte regeneration [8,9], when combined with 70% hepatectomy, leads to suppression of hepatocyte mitotic activity [9].…”

“…During the past few years, animal studies have demonstrated the effective use of mesna for the prevention or attenuation of I/R-induced injury in the liver [13,14], kidneys [15], stomach [14] and intestine [9,16]. Recent experimental data show a protective effect of mesna administration on hepatocyte regenerating activity in a rat model of partial hepatectomy under Pringle maneuver [7].…”

Bacterial Translocation in a Rat Model of Large Volume Hepatic Radiofrequency Ablation

“…Experimental studies performing open RFA in around 30% of liver mass have shown a rise of serum TNFa and IL-1b levels at 6 h after RFA in the pig [26], and of serum IL-6 at 24 and 48 h in the rat [42]. Furthermore, we have previously shown in a rabbit model that liver RFA triggers the initiation of liver regeneration process [43]. During the early phase of liver regeneration, TNFa and IL-1 serum levels are increased in order to prime resting hepatocytes for cell division [44].…”