Liver tissue partial pressure of oxygen and carbon dioxide during partial hepatectomy

Brooks, Adam; Eastwood, Jo; Beckingham, I. J.; Girling, Keith J.

doi:10.1093/bja/aeh112

Cited by 42 publications

(32 citation statements)

References 10 publications

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“…Unfortunately, no data are available on the in vivo oxygen partial pressure in rainbow trout liver tissue. In mammalian liver tissue, oxygen levels detected are in the range of 4-6·kPa, whereas at concentrations of 1.5-1.7·kPa, oxygen availability no longer matches consumption (Soller et al, 2001;Brooks et al, 2004). 'Physiologically normoxic' and 'critical' oxygen tensions correspond with 5-3% and 1% O 2 levels in gas phase, respectively.…”

Section: Discussionmentioning

confidence: 98%

“…Oxygen concentration measured under normoxic conditions in vivo in mammalian liver tissue ranges between 6 and 3·kPa, and shortterm anoxia does not result in complete tissue deoxygenation (e.g. Brooks et al, 2004;El Desoky et al, 1999). We have monitored transport and hydrolytic activities of the Na + /K + ATPase as a function of partial O 2 pressure (P O ∑) in the incubation medium in the range of 21-0.5·kPa.…”

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confidence: 99%

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Hypoxic responses of Na+/K+ ATPase in trout hepatocytes

Bogdanova

Grenacher

Nikinmaa

et al. 2005

Journal of Experimental Biology

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SUMMARY Reduction in oxygenation induces inhibition of Na+/K+ATPase in a number of cells and tissues, including hepatocytes. When not reversed, decrease in Na+/K+ pump activity leads to a gradual Na+ accumulation, cell swelling and death. However, when accompanied by suppression of dissipative cation pathways, it has also been shown to be a beneficial adaptive strategy used by some hypoxia-tolerant species to reduce ATP consumption during prolonged periods of anoxia. This study aims to investigate acute hypoxic responses of the Na+/K+ ATPase in primary cultures of trout hepatocytes. Gradual decrease in oxygenation was followed by an instantaneous transient dose-dependent downregulation of the Na+/K+ ATPase transport activity, but was without an effect on hydrolytic function of the enzyme. Hypoxia-induced inhibition of active K+ influx was reversed spontaneously when hypoxic incubation time exceeded 20 min. The stimulating effect of prolonged hypoxic exposure on the Na+/K+ pump is most probably secondary to hypoxia-induced activation of the Na+/H+ exchanger with the following Na+accumulation leading to Na+/K+ ATPase activation. Hypoxia-induced inhibition of the Na+/K+ pump was not caused by ATP depletion or global oxidative stress. However, local controlled production of reactive oxygen species seems to play an important role in hypoxia-induced regulation of the Na+/K+ ATPase. Treatment of cells with mercaptopropionyl glycine (MPG), a scavenger of \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \(\mathrm{O}\mathrm{H}^{.-}\) \end{document}, abolished hypoxia-induced inhibition of the Na+/K+ ATPase. Earlier on we have shown that activation of Na+/H+ exchanger under hypoxic conditions can be opposed by MPG treatment as well. Taken together our results suggest that regulation of both oxygen-sensitive transporters may be accomplished by local changes in free radical production.

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

Hypoxic responses of Na+/K+ ATPase in trout hepatocytes

Bogdanova

Grenacher

Nikinmaa

et al. 2005

Journal of Experimental Biology

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“…Additional effects, such as the transfer of vapor, were not considered in these calculations, 32 and the assumptions of air as the gas content may not be appropriate in some physiologic conditions, particularly in ischemic environments. 27 Nevertheless, these findings indicate diffusion in an important aspect of histotripsy-induced cavitation.…”

Section: Discussionmentioning

confidence: 93%

“…27 However, in vitro studies of histotripsy utilize phantoms of highly degassed agar to monitor bubble dynamics. 6,28,29 As noted in Fig.…”

Section: Representative Calculationmentioning

confidence: 99%

The influence of gas diffusion on bubble persistence in shock-scattering histotripsy

Bader

Bollen

2018

The Journal of the Acoustical Society of America

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Bubble cloud persistence reduces the efficacy of mechanical liquefaction with shock-scattering histotripsy. In this study, the contribution of gas transfer to bubble longevity was investigated in silico by solving the equations for bubble oscillations and diffusion in parallel. The bubble gas content increased more than 5 orders of magnitude during the expansion phase, arresting the inertial collapse. The residual gas bubble required more than 15 ms for passive dissolution post excitation, consistent with experimental observation. These results demonstrate gas diffusion is an important factor in the persistence of histotripsy-induced cavitation.

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“…The effect of an F i O 2 of 0.3 versus an F i O 2 of 1.0 on liver tissue oxygen tension was studied in 11 anesthetized patients undergoing hepatic resection [26] . Measurements were taken by a multi-analyte sensor (Paratrend , Diametrics Medical, UK) inserted under the liver capsule.…”

Section: Discussionmentioning

confidence: 99%

Effect of Supplemental Oxygen versus Dobutamine Administration on Liver Oxygen Tension in dPP-Guided Normovolemic Pigs

et al. 2009

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Background: Difference in pulse pressure (dPP) confirms adequate intravascular filling as a prerequisite for tissue perfusion. We hypothesized that both oxygen and dobutamine increase liver tissue oxygen tension (ptO₂). Methods: Eight anesthetized pigs received dPP-guided fluid management. Hepatic pO₂ was measured with Clark-type electrodes placed subcapsularly, and on the liver surface. Pigs received: (1) supplemental oxygen (F_iO₂ 1.0); (2) dobutamine 2.5 μg/kg/min, and (3) dobutamine 5 μg/kg/min. Data were analyzed using repeated-measures ANOVA followed by a Tukey post-test for multiple comparisons. ptO₂measured subcapsularly and at the liver surface were compared using the Bland-Altman plot. Results: Variation in F_iO₂ changed local hepatic tissue ptO₂ [subcapsular measurement: 39 ± 12 (F_iO₂ 0.3), 89 ± 35 mm Hg (F_iO₂ 1.0, p = 0.01 vs. F_iO₂ 0.3), 44 ± 10 mm Hg (F_iO₂ 0.3, p = 0.05 vs. F_iO₂ 1.0); surface measurement: 52 ± 35 (F_iO₂ 0.3), 112 ± 24 mm Hg (F_iO₂ 1.0, p = 0.001 vs. F_iO₂ 0.3), 54 ± 24 mm Hg (F_iO₂ 0.3, p = 0.001 vs. F_iO₂ 1.0)]. Surface measurements were widely scattered compared to subcapsular measurements (bias: –15 mm Hg, precision: 76.3 mm Hg). Dobutamine did not affect hepatic oxygenation. Conclusion: Supplemental oxygen increased hepatic tissue pO₂ while dobutamine did not. Although less invasive, the use of surface measurements is discouraged.

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Liver tissue partial pressure of oxygen and carbon dioxide during partial hepatectomy

Abstract: PtO2 and PtCO2 were measured directly using a Paratrend sensor in human liver tissue. During anaesthesia, changes in ventilation and liver blood flow caused predictable changes in PtCO2.

Cited by 42 publications

References 10 publications

Hypoxic responses of Na+/K+ ATPase in trout hepatocytes

Hypoxic responses of Na+/K+ ATPase in trout hepatocytes

The influence of gas diffusion on bubble persistence in shock-scattering histotripsy

Effect of Supplemental Oxygen versus Dobutamine Administration on Liver Oxygen Tension in dPP-Guided Normovolemic Pigs

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