Liver transplantation for cholangiocarcinoma

DeOliveira, Michelle L.

doi:10.1097/mot.0000000000000087

Cited by 33 publications

(11 citation statements)

References 37 publications

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“…CCA usually metastasize and cause relapse because it is commonly diagnosed in the advanced stage of the disease, and leads to poor prognosis [2][3][4]. Many studies have revealed that numerous genes are involved in CCA [5][6][7][8][9] and many therapeutic strategies have been developed to treat CCA [10][11][12]; however, the survival rate for CCA patients is still low [13]. In this case, the pathogenesis and progression of this malignancy remains to be investigated and new therapeutic targets need to be explored.…”

Section: Introductionmentioning

confidence: 99%

miR-191 Inhibition Induces Apoptosis Through Reactivating Secreted Frizzled-Related Protein-1 in Cholangiocarcinoma

Kang

Leng

Liu

et al. 2018

Cell Physiol Biochem

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Background/Aims: Cholangiocarcinoma (CCA) is one of the most common malignant tumors of the biliary tract originating from biliary epithelial cells. Although many therapeutic strategies have been developed to treat CCA, the survival rate for CCA patients is still quite low. Thus it is urgent to elucidate the pathogenesis of CCA and to explore novel therapeutic targets. miR-191 has been shown to be associated with many human solid cancers, but the function of miR-191 in CCA is still poorly understood. Methods: We first investigated the expression level of miR-191 in human CCA tissues and cell lines with quantitative real-time PCR (qRT-PCR). The effects of miR-191 on CCA cells were determined by Cell Counting Kit-8 assay, colony formation assay and acridine orange/ethidium bromide staining. Finally, we utilized qRT-PCR, western blot and luciferase reporter assays to verify the miR-191 target gene. Results: We showed that miR-191 was up-regulated in CCA cell lines and patients. Knockdown of miR-191 by transfection of its inhibitor sequence blocked RBE cells viability and induced apoptosis of RBE cells. Both qRT-PCR and western blot analysis showed that the secreted frizzled-related protein-1 (sFRP1) level was negatively correlated with that of miR-191. Luciferase assay validated that sFRP1 was a direct target of miR-191. Moreover, knockdown of miR-191 led to suppression of Wnt/β-catenin signaling activation. Co-transfection of sFRP1 small interfering RNA (siRNA) and miR-191 inhibitor re-activated the Wnt/β-catenin signaling pathway as detected by an increased level of β-catenin and phosphorylation of GSK-3β, and restored the expression of survivin and c-myc in RBE cells. Co-transfection of sFRP1 siRNA with miR-191 inhibitor restored the colony formation ability and viability of RBE cells. Conclusion: Taken together, our results demonstrate a novel insight into miR-191 biological function in CCA. Our findings suggest that miR-191 is a potential therapeutic target of CCA treatment.

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Section: Introductionmentioning

confidence: 99%

miR-191 Inhibition Induces Apoptosis Through Reactivating Secreted Frizzled-Related Protein-1 in Cholangiocarcinoma

Kang

Leng

Liu

et al. 2018

Cell Physiol Biochem

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“…75 Orthotopic liver transplantation failed as therapeutic approach for cholangiocarcinoma due to its unfavorable biology. 76 In 2002, Slakey published the first case of RFA in ICC, performing ablation and surgery in recurrent disease after liver surgery. 77 Similar case reports from various working groups followed in the next years.…”

Section: Intrahepatic Cholangiocellular Carcinomamentioning

confidence: 99%

Ablation in Primary Liver Tumors

2019

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Minimal invasive thermal ablation techniques have a key role in the treatment regimen of primary liver tumors. These relatively low-risk procedures are established in nonresectable liver tumors and even challenge the surgical approach, which is regarded as first-line treatment in eligible patients.The location and size of the liver tumor, the applied ablation, and guidance technique are crucial for treatment outcome. The confirmation of an adequate ablation zone including sufficient tumor overlap and a safety margin (A0 ablation analogous to R0 resection) is crucial to minimize local recurrence rates and improve survival. A variety of different ablation devices is available. The outcome of conventional computed tomography- or ultrasound-guided thermal ablation in small lesions is well comparable to surgery. However, in liver lesions with large diameter overlapping ablation zones are required to ensure an adequate ablation margin. Therefore, stereotactic techniques in combination with a multiple needle approach, three-dimensional trajectory planning, and image fusion for intraoperative treatment evaluation have been successfully introduced.

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“…Only 29% of these patients were surgical candidates, and in the subgroup of the remaining patients with stage I disease RFA was the only treatment strategy with improved survival (17). Orthotopic liver transplantation (OLT) is not considered to be a standard therapeutic approach for localized cholangiocarcinoma (18).…”

Section: Introduction Introductionmentioning

confidence: 99%

Minimally Invasive Locoregional Treatment of Intrahepatic Cholangiocellular Carcinoma

Putzer¹,

Schullian²,

Jaschke³

et al. 2018

SGO

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Surgical resection is considered first-line treatment for intrahepatic cholangiocellular carcinoma (ICC). In case of a unresectable tumor, chemotherapy is part of standardized treatment guidelines, despite dismal outcome. Technical advances in locoregional therapies such as radiofrequency ablation, microwave ablation, irreversible electroporation, transarterial chemoembolization and selective internal radiation therapy bear the promise for enhancing local tumor control, while reducing treatment associated morbidity and improving survival as well asquality of life. This review focuses on the latest improvements of minimally invasive locoregional therapies and their implementation in modern multimodality treatment protocols for ICC.

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Liver transplantation for cholangiocarcinoma

Abstract: The poor outcome of cholangiocarcinoma, irrespective of the therapy, justifies the search for novel approaches. Only selective patients with perihilar cholangiocarcinoma subjected to a neoadjuvant protocol may qualify for liver transplantation.

Cited by 33 publications

References 37 publications

miR-191 Inhibition Induces Apoptosis Through Reactivating Secreted Frizzled-Related Protein-1 in Cholangiocarcinoma

miR-191 Inhibition Induces Apoptosis Through Reactivating Secreted Frizzled-Related Protein-1 in Cholangiocarcinoma

Ablation in Primary Liver Tumors

Minimally Invasive Locoregional Treatment of Intrahepatic Cholangiocellular Carcinoma

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