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Kogawa, Katsuhisa; Muramatsu, Hirohito; Tanaka, Maki; Nishihori, Yoshiki; Hagiwara, Seiya; Kuribayashi, Kageaki; Nakamura, Kiminori; Koike, Kazuhiko; Sakamaki, Sumio; Niitsu, Yoshiro

doi:10.1023/a:1006633616629

Cited by 25 publications

(3 citation statements)

References 14 publications

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“…As described above, inhibition of tumor growth by Ge-132 might be observed only in cancer-derived cells lines, such as HepG2 cells. 29) We also investigated the possible effect of Ge-132 on intracellular oxidative stress induced by hydrogen peroxide. Previously, Ge-132 was reported to have an effect in increased oxidative stress models such as paraquart poisoning and lowdensity lipoprotein oxidation.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Activity of Ge-132, a Synthetic Organic Germanium, on Cultured Mammalian Cells

Wada

Hanyu

Nozaki

et al. 2018

Biological & Pharmaceutical Bulletin

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Ge-132 is a synthetic organic germanium that is used as a dietary supplement. The antioxidant activity of Ge-132 on cultured mammalian cells was investigated in this study. First, Ge-132 cytotoxicity on mammalian cultured cells was determined by measuring lactate dehydrogenase (LDH) levels. Ge-132 had no cytotoxic effect on three different cell lines. Second, the cell proliferative effect of Ge-132 was determined by measuring ATP content of whole cells and counting them. Ge-132 treatment of Chinese hamster ovary (CHO-K1) and SH-SY5Y cells promoted cell proliferation in a dose-dependent manner. Finally, antioxidant activity of Ge-132 against hydrogen peroxide-induced oxidative stress was determined by measuring the levels of intracellular reactive oxygen species (ROS) and carbonylated proteins. Pre-incubation of CHO-K1 and SH-SY5Y cells with Ge-132 suppressed intracellular ROS production and carbonylated protein levels induced by hydrogen peroxide. Our results suggest that Ge-132 has antioxidant activity against hydrogen peroxide-induced oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Antioxidant Activity of Ge-132, a Synthetic Organic Germanium, on Cultured Mammalian Cells

Wada

Hanyu

Nozaki

et al. 2018

Biological & Pharmaceutical Bulletin

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“…Scavenging of ROS leads to a reduction in metastasis, evidenced by the fact that catalase or superoxide dismutase effectively suppresses metastasis in an experimental metastasis model. [31][32][33] SKT may effectively prevent metastasis by scavenging ROS, although SKT is not likely to prevent tumor adhesion to endothelial cells as described above.…”

Section: Discussionmentioning

confidence: 99%

Suppressive Effect of Shichimotsu-koka-to (Kampo Medicine) on Pulmonary Metastasis of B16 Melanoma Cells.

Ohno

Ogihara

2002

Biological & Pharmaceutical Bulletin

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The lung, as well as the liver, is one of the organs most frequently involved in metastatic deposits from primary tumors.1-3) Tumor cell metastasis is a complex, multistep process that involves cell separation from the primary tumor, entry into the vascular and lymphatic systems, transport to and arrest within the microcirculation of distant organs, and extravasation. 4,5) The emergence of metastasis in organs distant from the primary tumor is the most devastating aspect of cancer. From this point of view, various inhibitors, such as inhibitors of angiogenesis and matrix metalloproteinase, are presently being developed as novel therapeutic drugs.Several Kampo medicines, such as Keishi-ka-kei-to, Juzen-taiho-to, Shimotsu-to, Unsei-in, Hochu-ekki-to, and Shosaiko-to have been so far reported to exhibit an antimetastatic effect.6-9) Among them, Juzen-taiho-to reduces liver metastasis by colon 26-L5 carcinoma cells as well as pulmonary metastasis by B16-BL6 melanoma cells, and its antimetastatic effect appears likely to be mediated by the activation of macrophages and/or T cells in the host immune system. 7) Keishi-ka-kei-to is also able to inhibit the pulmonary metastasis of B16 melanoma cells and its effect is in part due to the stimulation of CD8 ϩ T cells. 6) Shosaiko-to suppresses pulmonary metastasis induced by Lewis lung carcinoma cells, and the enhanced number of peritoneal macrophages and elevated binding of C3 cleavage products to macrophages after Shosaiko-to treatment may be related to its antimetastatic effects. 9) However, there have been few studies of Kampo medicines in the metastatic setting.Shichimotsu-koka-to (SKT), which is used to treat hypertension, especially essential and renal hypertension, and atherosclerosis, consists of seven medicinal plants (Paeoniae Radix, Angelicae Radix, Astragali Radix, Rehmanniae Radix, Cnidii Rhizoma, Phellodendri Cortex, and Uncariae Uncus et Ramulus), some of which have been studied to clarify their pharmacological actions. SKT shares four medicinal plants (Rehmanniae radix, Paeoniae radix, Cnidii rhizoma, Angelicae radix) with Shimotsu-to and Unsei-in, which exhibit antimetastatic effects against colon26-L5 carcinoma cells-induced liver metastasis. In addition, SKT shares five medicinal plants (Astragali Radix, Rehmanniae Radix, Paeoniae Radix, Cnidii Rhizoma, Angelicae Radix) with Juzentaiho-to, which also has antimetastatic effects. However, in addition to its antimetastatic effect, the pharmacological and biological actions of SKT have not been thoroughly investigated to date. A few reports found that SKT decreases systolic blood pressure and attenuates glomerular sclerotic lesions in the kidney after 4 weeks of treatment in salt-induced hypertensive Dahl strain rats, 10) and shows radical scavenging activity by increasing superoxide dismutase activity and decreasing xanthine oxidase activity in the brain. 11) SKT is able to quench superoxide anion as determined by electronspin resonance analysis in vitro. Considering that reactive oxygen species play an agg...

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“…On the other hand, scavenging of ROS leads to a reduction in metastasis, as evidenced by the facts that catalase or superoxide dismutase effectively suppresses metastasis in an experimental metastasis model, [5][6][7][8] and that ascorbic acid-2-O-phosphate-6-O-palmitate exerts antimetastatic activity by increasing intracellular anti-oxidant activity. 9) Taken together these results, ROS seem likely to affect various processes of metastasis, resulting in the development of metastasis.…”

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confidence: 99%