LLM-Domain B-GATA Transcription Factors Promote Stomatal Development Downstream of Light Signaling Pathways in <i>Arabidopsis thaliana</i> Hypocotyls

Klermund, Carina; Ranftl, Quirin L.; Diener, Julia; Bastakis, Emmanouil; Richter, René; Schwechheimer, Claus

doi:10.1105/tpc.15.00783

Cited by 67 publications

(72 citation statements)

References 58 publications

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“…The ICE proteins form heterodimers with SPCH, MUTE, and FAMA, for promoting stomatal cell fate transitions during this developmental process. It has been reported that SPCH transcription is induced by red light (Klermund et al, 2016). We observed that, while SPCH transcription is only marginally affected by light, its protein accumulation is markedly promoted in the light (Figures 6A to 6C).…”

Section: Photostabilization Of Ice Proteins During Stomatal Developmentsupporting

confidence: 52%

Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis

2017

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Stomata are epidermal openings that facilitate plant-atmosphere gas exchange during photosynthesis, respiration, and water evaporation. Stomatal differentiation and patterning are spatially and temporally regulated by the master regulators SPEECHLESS (SPCH), MUTE, and FAMA, which constitute a central gene regulatory network along with Inducer of Expression (ICE) transcription factors for this developmental process. Stomatal development is also profoundly influenced by environmental conditions, such as light, temperature, and humidity. Light induces stomatal development, and various photoreceptors modulate this response. However, it is unknown how light is functionally linked with the master regulatory network. Here, we demonstrate that, under dark conditions, the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) degrades ICE proteins through ubiquitination pathways in leaf abaxial epidermal cells in Accordingly, the ICE proteins accumulate in the nuclei of leaf abaxial epidermal cells in COP1-defective mutants, which constitutively produce stomata. Notably, light in the blue, red, and far-red wavelength ranges suppresses the COP1-mediated degradation of the ICE proteins to induce stomatal development. These observations indicate that light is directly linked with the ICE-directed signaling module, via the COP1-mediated protein surveillance system, in the modulation of stomatal development.

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Section: Photostabilization Of Ice Proteins During Stomatal Developmentsupporting

confidence: 52%

Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis

2017

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“…In the dark, when COP1 is stable, it degrades SCRM1 and SCRM2, independently of the YDA pathway [65]. Another report indicated that a light-induced GATA transcription factor of subfamily-B (B-GATA) promotes stomatal development in hypocotyls of A. thaliana [66,67]. B-GATA works upstream of SPCH/MUTE/FAMA, but downstream of and suppressed by PIFs [68].…”

Section: Stomata Developmentmentioning

confidence: 99%

“…B-GATA works upstream of SPCH/MUTE/FAMA, but downstream of and suppressed by PIFs [68]. B-GATAs promote SPCH expression by directly binding to its promoter region [66]. Arabidopsis PIF4 binds and suppresses SPCH, a master regulator of stomatal lineage initiation under high temperature [69].…”

Section: Stomata Developmentmentioning

confidence: 99%

Regulation of Photomorphogenic Development by Plant Phytochromes

Tripathi

Hoang

Han

et al. 2019

IJMS

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Photomorphogenesis and skotomorphogenesis are two key events that control plant development, from seed germination to flowering and senescence. A group of wavelength-specific photoreceptors, E3 ubiquitin ligases, and various transcription factors work together to regulate these two critical processes. Phytochromes are the main photoreceptors in plants for perceiving red/far-red light and transducing the light signals to downstream factors that regulate the gene expression network for photomorphogenic development. In this review, we highlight key developmental stages in the life cycle of plants and how phytochromes and other components in the phytochrome signaling pathway play roles in plant growth and development.

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“…Also, it appears that SPCH could bind to its own promoter and likely form a positive feedback for its own expression (Lau et al, 2014). The LLM-domain B-GATA transcription factor GNL1 (GNC-Like/cytokinin responsive GATA factor 1) interacted in vivo with the SPCH promoter and may be involved in red light-induced SPCH expression and stomatal development in hypocotyls (Klermund et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

IDD16 negatively regulates stomatal initiation via trans‐repression of SPCH in Arabidopsis

Lin

Feng

et al. 2019

Plant Biotechnology Journal

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Summary In Arabidopsis , the initiation and proliferation of stomatal lineage cells is controlled by SPEECHLESS ( SPCH ). Phosphorylation of SPCH at the post‐translational level has been reported to regulate stomatal development. Here we report that IDD 16 acts as a negative regulator for stomatal initiation by directly regulating SPCH transcription. In Arabidopsis , IDD 16 overexpression decreased abaxial stomatal density in a dose‐dependent manner. Time course analysis revealed that the initiation of stomatal precursor cells in the IDD 16 ‐ OE plants was severely inhibited. Consistent with these findings, the transcription of SPCH was greatly repressed in the IDD 16 ‐ OE plants. In contrast, IDD 16‐ RNA i transgenic line resulted in enhanced stomatal density, suggesting that IDD 16 is an intrinsic regulator of stomatal development. Ch IP analysis indicated that IDD 16 could directly bind to the SPCH promoter. Furthermore, Arabidopsis plants overexpressing IDD 16 exhibited significantly increased drought tolerance and higher integrated water use efficiency ( WUE ) due to reduction in leaf transpiration. Collectively, our results established that IDD 16 negatively regulates stomatal initiation via trans‐repression of SPCH , and thus provide a practical tool for increasing plant WUE through the manipulation of IDD 16 expression.

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LLM-Domain B-GATA Transcription Factors Promote Stomatal Development Downstream of Light Signaling Pathways in Arabidopsis thaliana Hypocotyls

Cited by 67 publications

References 58 publications

Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis

Light Inhibits COP1-Mediated Degradation of ICE Transcription Factors to Induce Stomatal Development in Arabidopsis

Regulation of Photomorphogenic Development by Plant Phytochromes

IDD16 negatively regulates stomatal initiation via trans‐repression of SPCH in Arabidopsis

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