LncIMF2 promotes adipogenesis in porcine intramuscular preadipocyte through sponging MiR-217

Yi, Xudong; He, Zhaozhao; Tian, Tingting; Kou, Zhongyun; Pang, Wei

doi:10.1080/10495398.2021.1956509

Cited by 15 publications

(13 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At present, many studies have reported the regulatory effect of non-coding RNAs, including circRNA, on animal fat metabolism. [22][23][24] CircRNA is an ideal biomarker with high application value. Understanding the role of circRNA in porcine adipogenesis will be of great significance for pork quality and breed improvement.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

CircHOMER1 inhibits porcine adipogenesis via the miR‐23b/SIRT1 axis

et al. 2023

The FASEB Journal

View full text Add to dashboard Cite

Fat deposition is critical to pork quality. However, the mechanism of fat deposition remains to be elucidated. Circular RNAs (circRNAs) are ideal biomarkers and are involved in adipogenesis. Here, we investigated the effect and mechanism of circHOMER1 on porcine adipogenesis in vitro and in vivo. Western blotting, Oil red O staining, and HE staining were used to assess the function of circHOMER1 in adipogenesis. The results showed that circHOMER1 inhibited adipogenic differentiation of porcine preadipocytes and suppressed adipogenesis in mice. Dual-luciferase reporter gene, RIP, and pull-down assays demonstrated that miR-23b directly bound to circHOMER1 and the 3′-UTR of SIRT1. Rescue experiments further illustrated the regulatory relationship among circHOMER1, miR-23b, and SIRT1. Conclusively, we demonstrate that circHOMER1 plays an inhibitory role in porcine adipogenesis through miR-23b and SIRT1. The present study revealed the mechanism of porcine adipogenesis, which may be helpful to improve pork quality.

show abstract

Section: Discussionmentioning

confidence: 99%

“…At present, many studies have reported the regulatory effect of non‐coding RNAs, including circRNA, on animal fat metabolism 22–24 . CircRNA is an ideal biomarker with high application value.…”

Section: Discussionmentioning

confidence: 99%

CircHOMER1 inhibits porcine adipogenesis via the miR‐23b/SIRT1 axis

et al. 2023

The FASEB Journal

View full text Add to dashboard Cite

show abstract

“…Meanwhile, a basic source gene-circRNA-miRNA connective network ( Figure 5 ) was constructed, which can provide a reference for subsequent studies on the function of these DECs. As shown in Figure 5 , some of DECs’ target miRNA, such as miR-127, miR-217, and miR-183, have been reported to affect lipid metabolism or adipogenesis [ 11 , 54 , 55 ]. The results suggestthat circRNAs may be involved in the process of adipogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Studies had proven that several lncRNAs and circRNAs are involved in adipogenesis. For instance, IncIMF2 acts as a molecular sponge for microRNA-217, which has been found associated with adipogenesis, thereby affecting the expression of the miR-217 target gene [ 11 ]. LncRNA IRLnc influence IMF decomposition by regulating the expression of nuclear receptor subfamily 4 group A member 3 [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Differentially Expressed mRNAs, lncRNAs and circRNAs Related to Intramuscular Fat Deposition in Laiwu Pigs

Zhao

Wang

et al. 2022

Genes

View full text Add to dashboard Cite

Long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) are important classes of small noncoding RNAs that can regulate numerous biological processes. To understand the role of message RNA (mRNAs, lncRNAs and circRNAs) in the regulation of intramuscular fat (IMF) deposition, in this study the expression profiles of longissimus dorsi (LD) muscle from six Laiwu pigs (three with extremely high and three with extremely low IMF content) were sequenced based on rRNA-depleted library construction. In total, 323 differentially expressed protein-coding genes (DEGs), 180 lncRNAs (DELs) and 105 circRNAs (DECs) were detected between the high IMF and low IMF groups. Functional analysis indicated that most DEGs, and some target genes of DELs, were enriched into GO terms and pathways related to adipogenesis, suggesting their important roles in regulating IMF deposition. In addition, 12 DELs were observed to exhibit a positive relationship with stearoyl-CoA desaturase (SCD), phosphoenolpyruvate carboxykinase 1(PCK1), and adiponectin (ADIPOQ), suggesting they are highly likely to be the target genes of DELs. Finally, we constructed a source gene-circRNA-miRNA connective network, and some of miRNA of the network have been reported to affect lipid metabolism or adipogenesis. Overall, this work provides a valuable resource for further research and helps to understand the potential functions of lncRNAs and circRNAs in IMF deposition.

show abstract

“…LncRNA Blnc1 is one of the conserved lncRNAs in the process of adipocyte differentiation It can promote adipocyte metabolism by binding to protein Zbtb7b (19). With the research of lncRNAs combined with bioinformatics technology, more and more lncRNAs have been identified with potential functions to regulate adipocyte metabolism, such as lncRNA uc.333 (20), lncIMF2 (21), MALAT1 (22), lncPRDM16 (23), etc. However, there are some limitations for those studies.…”

Section: Introductionmentioning

confidence: 99%

Identification and analysis of lncRNA, miRNA and mRNA related to subcutaneous and intramuscular fat in Laiwu pigs

et al. 2023

View full text Add to dashboard Cite

BackgroundLong non-coding RNAs (lncRNAs) regulate adipocyte differentiation and metabolism, However, their function on subcutaneous and intramuscular adipose tissues in pigs is unclear. Intramuscular fat (IMF) is an important indicator for evaluating meat quality. Breeds with high IMF content are often accompanied by high subcutaneous fat (SCF), which severely affects the meat rate of pigs. It is of great significance for porcine breeding to study the mechanism of lncRNA related to adipogenesis and lipid metabolism.MethodsWe identified differentially expressed lncRNAs, miRNAs and mRNAs in subcutaneous and intramuscular adipose tissues in three female Laiwu pigs by deep RNA-sequencing(|log2foldchange|≥1, P_value ≤ 0.05). The gene expression profiles of IMF and SCF in Laiwu pigs were comparatively analyzed by Bioinformatics methods to identify key lncRNAs, miRNAs, and mRNAs associated with lipid metabolism and adipogenesis.ResultsA total of 1209 lncRNAs (DElncRNAs), 286 miRNAs (DEmiRNAs), and 1597 mRNAs (DEgenes) were differentially expressed between two types of adipose. Among them, 17 DElncRNAs and 103 target genes play a role in the co-expression network, as well as 59 DElncRNAs, 44 DEmiRNAs, and 88 DEgenes involved in ceRNA network. In GO(Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis of DElncRNAs their target genes involved in many adipogenesis and lipid metabolism biological processes and signaling pathways, such as PPAR signaling pathway, Wnt signaling pathway, MAPK signaling pathway.ConclusionsBy constructing co-expression and ceRNAs network we found that Wnt signaling pathway play a critical regulatory role in intramuscular adipogenesis and lipid accumulation in Laiwu pigs. TCONS_00006525, TCONS_00046551 and TCONS_00000528 may target WNT5A, WNT10B and FDZ3 in co-expression network, TCONS_00026517 and other lncRNAs regulate the expression of PPARG, RXRG and SCD in ceRNA network, and were involved in Wnt signaling pathway. This study provides a theoretical basis for further understanding the post-transcriptional regulation mechanism of meat quality formation, predicting and treating diseases caused by ectopic fat.

show abstract

LncIMF2 promotes adipogenesis in porcine intramuscular preadipocyte through sponging MiR-217

Cited by 15 publications

References 43 publications

CircHOMER1 inhibits porcine adipogenesis via the miR‐23b/SIRT1 axis

CircHOMER1 inhibits porcine adipogenesis via the miR‐23b/SIRT1 axis

Comprehensive Analysis of Differentially Expressed mRNAs, lncRNAs and circRNAs Related to Intramuscular Fat Deposition in Laiwu Pigs

Identification and analysis of lncRNA, miRNA and mRNA related to subcutaneous and intramuscular fat in Laiwu pigs

Contact Info

Product

Resources

About