LncMSEN1, a mantle-specific LncRNA participating in nacre formation and response to polyI:C stimulation in pearl oyster Pinctada fucata martensii

Zheng, Zhe; Li, Wenhui; Xu, Jin; Xie, Bingyi; Yang, Modong; Huang, Huajie; Li, Huishan; Wang, Qingheng

doi:10.1016/j.fsi.2019.12.015

Cited by 15 publications

(7 citation statements)

References 32 publications

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“…However, miRNAs have been described and/or analyzed in a few mollusk species during the past decade [ 53 , 54 , 55 , 56 , 57 , 58 , 59 ], including M. galloprovincialis [ 26 , 60 ]. On the other hand, lncRNAs have been less studied, but some recent publications have reported the identification, expression profiles, and even the potential functions of some mollusk lncRNAs [ 29 , 61 , 62 , 63 , 64 , 65 , 66 ]. However, the identification and RNA-Seq analysis of M. galloprovincialis lncRNAs is limited to a recent publication describing the lncRNAs modulated in gills after V. splendidus bath infection [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…At the same time, although the functional classification of lncRNAs remains very challenging, these transcripts can be divided into two groups based on the location at which the lncRNA acts: trans-acting and cis-acting lncRNAs [ 27 , 68 ]. Whereas trans-acting lncRNAs are transcribed, processed, and then migrate to distal locations to exert their function, cis-acting lncRNAs regulate the expression of neighboring genes, and these cis-acting lncRNAs constitute a substantial proportion of lncRNAs with an assigned function [ 66 ]. Therefore, in the absence of experimental evidence, the processes affected by an array of lncRNAs modulated under certain conditions could be at least partially estimated based on the function of their neighboring genes.…”

Section: Discussionmentioning

confidence: 99%

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Differential Expression of Long Non-Coding RNA (lncRNA) in Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under Immune Stimuli

Pereiro

Moreira

Novoa

et al. 2021

Genes

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The Mediterranean mussel is one of the most economically relevant bivalve mollusk species in Europe and China. The absence of massive mortalities and their resistance to pathogens affecting other cultured bivalves has been under study in recent years. The transcriptome response of this species to different immune stimuli has been extensively studied, and even the complexity of its genome, which has recently been sequenced, has been suggested as one of the factors contributing to this resistance. However, studies concerning the non-coding RNA profiles remain practically unexplored—especially those corresponding to the lncRNAs. To the best of our knowledge, this is the second characterization and study of lncRNAs in this bivalve species. In this work, we identified the potential repertoire of lncRNAs expressed in mussel hemocytes, and using RNA-Seq we analyzed the lncRNA profile of mussel hemocytes stimulated in vitro with three different immune stimuli: LPS, poly I:C, and β-glucans. Compared to unstimulated hemocytes, LPS induced the highest modulation of lncRNAs, whereas poly I:C and β-glucans induced a similar discrete response. Based on the potential cis-regulatory activity of the lncRNAs, we identified the neighboring protein-coding genes of the regulated lncRNAs to estimate—at least partially—the processes in which they are implicated. After applying correlation analyses, it seems that—especially for LPS—the lncRNAs could participate in the regulation of gene expression, and substantially contribute to the immune response.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Differential Expression of Long Non-Coding RNA (lncRNA) in Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under Immune Stimuli

Pereiro

Moreira

Novoa

et al. 2021

Genes

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“…A previous study demonstrated that lncRNA is involved in diverse pathological processes of tumorigenesis and development at different levels ( 16 ). In addition, lncRNA has been demonstrated to participate in a number of important physiological and pathological processes, such as cell proliferation ( 17 ), differentiation ( 18 ), tissue formation ( 19 ) and organ formation ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

Maternally expressed 3 inhibits the biological activity of oral squamous cell carcinoma SCC25 and CAL27 cell lines

Wang

Li²,

Qiu

et al. 2021

Oncol Lett

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The abnormal expression of long non-coding RNA (lncRNA) maternally expressed 3 (MEG3) is closely related to several tumor diagnosis and progression, such as endometrial carcinoma and ovarian cancer. However, the role of MEG3 in oral squamous cell carcinoma (OSCC) is rarely reported. The current study aimed to evaluate the expression of lncRNA MEG3 in OSCC tissues and cell lines and its effect on the biological behavior of OSCC cell lines. The expression of lncRNA MEG3 in the OSCC tissues and cell lines was detected by reverse transcription-quantitative (RT-q) PCR. The relationship between MEG3 expression and the clinicopathologic characteristics and prognosis of patients with OSCC was analyzed. The lncRNA MEG3 overexpression plasmid and control plasmid were transfected into SCC25 and CAL27 cell lines using the lipofectin method. MTT assay was performed to detect the growth and proliferation of the cell lines. Transwell chamber test was used to detect changes in cell migration and invasion. Flow cytometry was employed to detect changes in apoptosis. Western blotting and RT-qPCR were conducted to detect the expression of the p53 gene. The expression of lncRNA MEG3 in the OSCC tissues and cell lines was significantly compared with normal tissues and cell lines, respectively. The expression level of MEG3 was related to clinical stage, lymph node metastasis, distant metastasis and survival status. Overexpression of lncRNA MEG3 inhibited the proliferation, migration, and invasion of SCC25 and CAL27 cell lines, induced apoptosis and promoted the expression of p53 gene. lncRNA MEG3 played the role of a tumor inhibitor gene and significantly inhibited the biological activity of OSCC cell lines, which may provide a novel idea for molecular targeted therapy of OSCC.

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“…Then, LncRNA is a cluster of epigenetic regulators that have an important role in biomineralization, immunity, and stress resistance (Yu et al, 2016;Feng et al, 2018;Huang et al, 2018;Zheng et al, 2019). Previously, LncRNAs had been reported to play a role in biomineralization of pearl oysters, such as LncMSEN1, LncMSEN2, and LncRunt (Zheng et al, 2019;Zheng et al, 2020a;Zheng et al, 2020b). In this study, we cloned a novel LncRNA that is highly expressed in the mantle…”

Section: Discussionmentioning

confidence: 92%

“…Also, LncRNAs are reported to participate in controlling shell formation and body growth by affecting pigmentation, shell matrix gene expression, and glycogen accumulation (Sun and Feng, 2018). In addition, our previous study proposed that the biomineralization process and defense function of the mantle tissue are crosslinked and regulated by LncRNAs in the pearl oyster (Zheng et al, 2020b;Zheng et al, 2020c). As mentioned above, changes in environmental conditions could induce responses at multiple levels in mollusk species, including affecting the biomineralization process.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA LncMPEG1 responds to multiple environmental stressors by affecting biomineralization in pearl oyster Pinctada fucata martensii

Cai

Xie

et al. 2022

Front. Mar. Sci.

Self Cite

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Marine environmental change directly affects bivalve growth and survival. Exoskeleton formation, the main energy dissipation in the physiological metabolism, typically reflects the body growth of the bivalve. However, how bivalves regulate the biomineralization of the exoskeleton under environmental stressors is not yet clear. Long non-coding RNA regulates various life processes through complex mechanisms in vertebrates and invertebrates. In this research, we cloned the complete sequence of a mantle-specific expressed long non-coding RNA (designated as LncMPEG1) from a pearl oyster, Pinctada fucata martensii. A quantitative real-time PCR analysis showed that LncMPEG1 expression was significantly high in early umbo larvae and juveniles, which would be the critical periods of shell development. LncMPEG1 was identified in the outer epithelium of the middle fold from the mantle edge, mantle pallial, and mantle center by using in situ hybridization. Additionally, the expression of LncMPEG1 was stimulated by shell damage, alien invasion, heat and cold temperature stress, and hypoxia stress. In the mantle, a decreased in LncMPEG1 expression was detected by RNA interference, which can cause the irregular growth of crystals on the inner surface of the prismatic layer and nacre in the shells. Therefore, we propose that LncMPEG1 could be a key regulator in biomineralization and responds to environmental stress in the mantle .

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LncMSEN1, a mantle-specific LncRNA participating in nacre formation and response to polyI:C stimulation in pearl oyster Pinctada fucata martensii

Cited by 15 publications

References 32 publications

Differential Expression of Long Non-Coding RNA (lncRNA) in Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under Immune Stimuli

Differential Expression of Long Non-Coding RNA (lncRNA) in Mediterranean Mussel (Mytilus galloprovincialis) Hemocytes under Immune Stimuli

Maternally expressed 3 inhibits the biological activity of oral squamous cell carcinoma SCC25 and CAL27 cell lines

Long non-coding RNA LncMPEG1 responds to multiple environmental stressors by affecting biomineralization in pearl oyster Pinctada fucata martensii

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