lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis

Abstract

Background. Previous studies have demonstrated that lncRNAs play functional roles in regulating cancer cell proliferation, invasion, and apoptosis. Recent studies confirmed that lncRNA 00312 has important biological functions in lung and colorectal cancer. However, the role of lncRNA 00312 in renal cell carcinoma (RCC) remains unclear. Our aim was to explore the function of lncRNA 00312 in RCC and its potential molecular mechanism. Methods. RCC cell lines A498 and ACHN were used as in vitro models in this study. RT-PCR was performed to determine lncRNA 00312, miR-34a-5p, and ASS1 mRNA expression. Proliferation and invasion were examined by CCK-8 and Transwell assay to confirm the function role of lncRNA 00312. Western blot analysis was used to examine the expression of apoptotic proteins Bax and Bcl-2. Results. lncRNA was significantly downregulated in RCC cells such as A498 and ACHN; the expression of lncRNA 00312 in RCC tissues was significantly lower than that in adjacent normal tissues. Patients with low expression of lncRNA 00312 have worse prognosis regarding pathological grade, tumor size, and TNM stage. Overexpression of lncRNA 00312 suppressed A498 and ACHN cell proliferation and invasion, while promoting apoptosis. Our study found that miR-34a-5p had the potential binding site with lncRNA 00312 and revealed the role of miR-34a-5p in RCC. Furthermore, we confirmed that lncRNA 00312 played its role with the participation of ASS1 and miR-34a-5p. Conclusion. lncRNA 00312 can inhibit RCC proliferation and invasion and promote apoptosis in vitro by suppressing miR-34a-5p and overexpressing ASS1. Our study demonstrated that the lncRNA 00312/miR-34a-5p/ASS1 axis may play a functional role in the progression of RCC; lncRNA 00312 abundance is a prognostic factor candidate for RCC survival, which provides new insights for RCC clinical treatment.

Long noncoding RNA SNHG4 promotes renal cell carcinoma tumorigenesis and invasion by acting as ceRNA to sponge miR-204-5p and upregulate RUNX2

“…In recent decades, many studies have found the critical function of lncRNAs in many types of human cancer, including RCC [ 11 , 26 , 27 ]. This evidence is of benefit to clarify the mechanistic basis of RCC.…”

“…For example, enhanced lncRNA ARSR expression was shown in RCC‐initiating cells, related with dismal survival, and contributed to the sunitinib resistance in RCC patients [ 10 ]. Results from Zeng et al showed that lncRNA 00312 could prohibit RCC cell proliferation and migration and boost apoptosis in vitro by repressing miR-34a-5p and enhancing expression of ASS1 [ 11 ]. Some lncRNAs named small nucleolar RNA host genes (SNHGs), which can encode small nucleolar RNAs (snoRNAs) [ 12 , 13 ].…”

LncRNA SNHG17 Promotes Tumor Progression and Predicts Poor Survival in Human Renal Cell Carcinoma via Sponging miR-328-3p

“…[6,7] LncRNAs exert oncogenic or suppressive functions in various human cancers, including RCC. [8][9][10] LncRNA small nuclear RNA host gene 17 (SNHG17) is located on 20q11. 23, with a 1,186 nt in length.…”

lncRNA-RMST functioned as a SOX2 transcription co-regulator to regulate miR-1251 in the progression of Hirschsprung’s disease

“…For instance, lncRNA TPTEP1 could inhibit the non-small cell lung cancer (NSCLC) cells to proliferate through abating miR-328-5p expression (Cao et al, 2020). In addition, in renal cell carcinoma, lncRNA00312 attenuated cell proliferation and migration obviously (Zeng et al, 2020). However, the study about lncRNA functioned in HSCR is rarely reported.…”