LncRNA AK077216 is downregulated in diabetic retinopathy and inhibited the apoptosis of retinal pigment epithelial cells by downregulating miR-383

Abstract

LncRNA AK077216 is a novel lncRNA with critical role in osteoclastogenesis and bone resorption. Our preliminary RNA-seq data suggested its downregulated expression pattern in diabetic retinopathy (DR). We therefore further investigated the function of AK077216 in DR. Plasma samples used in this study were derived from blood, which was extracted from 60 diabetic retinopathy patients. AK077216 in plasma of DR patients, diabetic patients (DB) and controls (Control) was detected by RT-qPCR. ROC curve analysis was performed to evaluate the diagnostic value of plasma AK077216 for DR. It was found that plasma AK077216 was downregulated only in DR patients but not in diabetic patients (no obvious complications) comparing to healthy controls. Downregulation of AK077216 distinguished DR patients from diabetic patients and healthy controls. High glucose treatment failed to significantly affect AK077216 expression in cells of human retinal pigment epithelial cell line ARPE-19. MiR-383 was inversely correlated with AK077216 in DR patients. AK077216 overexpression caused miR-383 downregulation, but miR-383 overexpression did not significantly alter AK077216 expression. AK077216 overexpression inhibited ARPE-19 cell apoptosis, while miR-383 overexpression played an opposite role and attenuated the effects of AK077216 overexpression. Therefore, AK077216 is downregulated in diabetic retinopathy and inhibited the apoptosis of ARPE-19 cells by downregulating miR-383.

Mbd2 Mediates Retinal Cell Apoptosis by Targeting the lncRNA Mbd2-AL1/miR-188-3p/Traf3 Axis in Ischemia/Reperfusion Injury

“…A recent study has reported that Mbd2 upregulates miR-301a-5p to mediate renal cell apoptosis during vancomycin treatment. 10 Although many studies reported that lncRNAs were involved in the progression of retinal diseases, [26][27][28] it is still unclear whether Mbd2 regulates the expression of lncRNAs. Here, we found that lncRNA Mbd2-AL1 was a direct target of Mbd2, as suggested by our results.…”

MicroRNA Expression Analysis of Mice Retinas with Oxygen-Induced Retinopathy by RNA Sequencing

“…miR-383-5p was downregulated in three studies. It was reported that miR-383 inhibits the apoptosis of retinal pigment epithelial cells in diabetic retinopathy [ 39 ] and inhibits proliferation, migration, and angiogenesis of endothelial cells exposed to gliomas through VEGF-mediated FAK and Src signaling pathways [ 40 ]. In contrast, miR-211 was identified in two independent studies, but in opposite directions.…”

Importance of the Use of Oxidative Stress Biomarkers and Inflammatory Profile in Aqueous and Vitreous Humor in Diabetic Retinopathy

“…H-19, a lncRNA that prevents glucose-induced endothelial mesenchymal transition in the retina, is downregulated in hyperglycemic conditions leading to activation of endothelial transition via TFGF-beta [288]. A recent study has found that lncRNA AK077216 is downregulated in subjects with DR independently from glycemic conditions, this lncRNA is able to inhibit ARPE-19 cells apoptosis via inhibition of miR-383 proapototic function [289]. The proliferation promoting lncRNA BANCR has also been found overexpressed in patients with DR which may play a role as a potential therapeutic target [290,291].…”