lncRNA C22orf32-1 contributes to the tumorigenesis of nasopharyngeal carcinoma

Nie, Guohui; Zhao, Li; Duan, Hongfang; Luo, Liang; Hu, Hongyi; Yang, Weiqiang; Nie, Lei; Zhu, Rufei; Chen, Xiaofan; Zhang, Wei

doi:10.3892/ol.2017.6021

Cited by 17 publications

(14 citation statements)

References 38 publications

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“…A growing number of studies have indicated that lncRNAs can determine some cellular processes in cancer, including proliferation, metastasis, and differentiation (Eades et al, ; Jiang et al, ). Many earlier studies have presented that lncRNAs are associated with carcinogenesis, metastasis, prognosis, and diagnosis, and function as oncogenes or cancer suppressor genes (Chen et al, ; Jen et al, ; Nie et al, ). Herein, more studies are required to elucidate the biological and molecular mechanisms of lncRNAs on cancer.…”

Section: Discussionmentioning

confidence: 99%

LncRNA DGCR5 promotes lung adenocarcinoma (LUAD) progression via inhibiting hsa‐mir‐22‐3p

Dong

Ren

Jin

et al. 2017

Journal Cellular Physiology

141

135

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Long non-coding RNAs (lncRNAs) serve critical roles in the pathogenesis of various cancers, including lung adenocarcinoma (LUAD). Herein, in this study, we aimed to investigate the biological and clinical significance of lncRNA DiGeorge syndrome critical region gene 5 (DGCR5) in LUAD. It was observed that DGCR5 was upregulated in LUAD tissues and LUAD cell lines. Inhibition of DGCR5 can prevent LUAD progression via playing anti-apoptosis roles. Both mRNA expression and protein levels of BCL-2 were increased by DGCR5 downregulation while reversely BAX was increased. Additionally, a novel microRNA target of DGCR5, hsa-mir-22-3p was identified through bioinformatics search and confirmed by dual-luciferase reporter system. Gain and loss-of-function studies were performed to verify whether DGCR5 exerts its biological functions through regulating hsa-mir-22-3p in vitro. Overexpression of DGCR5 was able to reverse the tumor inhibitory effect of hsa-mir-22-3p mimics. Furthermore, in vivo tests tumor xenografts were established to detect the function of DGCR5 in LUAD tumorigenesis. Downregulated DGCR5 expression was greatly associated with smaller tumor size, implying a favorable prognosis of LUAD patients. Taken these together, DGCR5 could be considered as a prognostic biomarker and therapeutic target in LUAD diagnosis and treatment.

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Section: Discussionmentioning

confidence: 99%

LncRNA DGCR5 promotes lung adenocarcinoma (LUAD) progression via inhibiting hsa‐mir‐22‐3p

Dong

Ren

Jin

et al. 2017

Journal Cellular Physiology

141

135

View full text Add to dashboard Cite

show abstract

“…In this content, it has been reported that several lncRNAs are related with NPC pathogenesis. For instance, lncRNA-LINC00460 facilitated NPC tumorigenesis through sponging miR-149-5p to up-regulate IL-6 (10) , lncRNA FAM226A promoted NPC tumorigenesis and metastasis by acting as ceRNA to sponge miR-590-3p/miR- 1275 and up-regulate ITGB3 (17) , lncRNA HOTAIR contributed to the tumorigenesis of NPC via up-regulating FASN (11) , lncRNA LINC01133 mediated NPC tumorigenesis by binding to YBX1 (15) , lncRNA C22 or f32-1 contributed to the tumorigenesis of NPC (12) , lncRNA PXN-AS1-L promoted the malignancy of NPC cells via up-regulation of SAPCD2 (13) , and the lncRNA PVT1 regulated NPC cell proliferation via activating the KAT2A acetyltransferase and stabilizing HIF-1a (14) . Consistent with these previous reports, in the current study, we demonstrated that suppression tumor suppressors in variety kinds of cancers (23) .…”

Section: Discussionmentioning

confidence: 99%

“…In this regard, several lncRNAs including EWSAT1 (9) , LINC00460 (10) , HOTAIR (11) , C22orf32-1 (12) , PXN-AS1-L (13) , PVT1 (14) , LINC01133 (15) , DANCR (16) , and FAM225A (17) haven been reported to be associated with NPC tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

Role of lncRNA-ENST00000412010 in regulating nasopharyngeal cancer cell survival

Cheng¹,

Hu²,

Lu³

et al. 2020

Rhin

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Background: Nasopharyngeal carcinoma (NPC) is a rare tumor with highly recurrent and lack of effective treatment. Long noncoding RNAs (lncRNAs) have been reported to play roles in various cancers including NPC. Methods: In the current study, two cell lines of NPC (CNE-2Z and 5-8F cells) were transfected with short hairpin RNA (shRNA) targeting lncRNA-ENST00000412010 (shlncRNA) or control shRNA (shControl). Cell proliferation, survival, in vitro colony formation, and in vivo xenograft tumor formation were then investigated. Results: The study found that cells transfected with shlncRNA grew significantly slower than the cells transfected with shControl as measured on day 5; increased in Annexin V expression; decreased in colony formation; and smaller in xenograft tumor size on day 45. Expression of DNA damage-inducible transcript 3, dual specificity protein phosphatase 5, insulin receptor substrate 1, interleukin-6, and tribbles homolog 3 genes was significantly up-regulated in the cells transfected with shlncRNA, while gene expression of matrix metalloproteinase-7 and cyclin-dependent kinase 4 inhibitor B was significantly down-regulated in the cells transfected with shlncRNA. Immunoblotting assay confirmed DUSP5 protein was significantly increased while proteins of MMP-7 and CDKN2B were significantly lower in the cells lacking lncRNA than that of the control cells. Conclusions: These findings suggested that lncRNA-ENST00000412010 plays a role in modulating NPC survival and tumorigenesis through regulating molecules associated with cell cycle and protein phosphatase.

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“…In the past decade, studies have uncovered the role of lncRNAs in carcinogenesis, suggesting that these genes may be used as promising biomarkers in cancer ( 4 – 6 ). Among them, lncRNA ANRIL is associated with an unfavorable prognosis and aggravates invasion in malignant OC ( 5 ), whereas lncRNA homeobox (HOX) A11-antisense suppresses the oncogenic phenotype of epithelial OC ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

LncRNA HOTTIP promotes the proliferation and invasion of ovarian cancer cells by activating the MEK/ERK pathway

Liu

et al. 2020

Mol Med Rep

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recent studies have revealed that long non-coding rnas (lncrnas) serve important roles in carcinogenesis and that this type of gene may be used as biomarkers in cancer. a high level of lncrna HoXa distal transcript antisense rna (HoTTiP) is associated with unfavorable prognosis for patients with ovarian cancer (oc), but the mechanism of HoTTiP involved in oc development remains to be elucidated. The present study aimed to investigate the mechanism of HoTTiP in metastasis-associated oc cell behaviors. HoTTiP levels in ovarian cells were quantified by reverse transcription-quantitative Pcr, cell proliferation was analyzed by colony formation assay, and apoptosis was assessed by flow cytometry. Cell migratory and invasive abilities were evaluated by wound healing and Transwell assays, respectively. The expression levels of mitogen-activated protein kinase kinase (MeK)/erK pathway-associated proteins were detected by western blotting. The results demonstrated that knockdown of HoTTiP in OC cells significantly reduced the phosphorylation levels of MeK and erK, inhibited the proliferation and invasion of oc cells and promoted their apoptosis. Furthermore, the effects of HoTTiP on cell migration and invasion were partly associated with the epithelial-mesenchymal transition (eMT) process. Proliferation, invasion and eMT of oc cells were enhanced following overexpression of HoTTiP; however, these effects were reversed by the MeK/erK pathway inhibitor u0126. in conclusion, HoTTiP was demonstrated to promote the proliferation, migration and invasion of oc cells by activating the MeK/erK pathway. Therefore, HoTTiP may serve as a potential therapeutic target for oc.

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lncRNA C22orf32-1 contributes to the tumorigenesis of nasopharyngeal carcinoma

Cited by 17 publications

References 38 publications

LncRNA DGCR5 promotes lung adenocarcinoma (LUAD) progression via inhibiting hsa‐mir‐22‐3p

LncRNA DGCR5 promotes lung adenocarcinoma (LUAD) progression via inhibiting hsa‐mir‐22‐3p

Role of lncRNA-ENST00000412010 in regulating nasopharyngeal cancer cell survival

LncRNA HOTTIP promotes the proliferation and invasion of ovarian cancer cells by activating the MEK/ERK pathway

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