lncRNA FEZF1‑AS1 promotes migration, invasion and epithelial‑mesenchymal transition of retinoblastoma cells by targeting miR‑1236‑3p

Abstract

long non-coding rnas (lncrnas) and micrornas (mirs) have been reported to regulate disease progression in numerous types of disease, including retinoblastoma (rb). Therefore, the present study aimed to investigate the effects of the lncRNA FEZ family zinc finger 1 antisense RNA 1 (FEZF1-AS1) on Rb and to determine its possible mechanism of action. reverse transcription-quantitative Pcr and western blot analysis were conducted to detect the gene or protein expression. cell counting Kit-8, wound healing and transwell invasion assays were performed to estimate the capabilities of cell viability, invasion and migration. The potential association between FEZF1-AS1 and miR-1236-3p in Y79 cells was measured via dual-luciferase reporter assay. The results of the present study revealed that the levels of FEZF1-AS1 were significantly upregulated in different Rb cell lines, with the most prominent upregulation observed in Y79 cells. In addition, the cell viability, invasive and migratory abilities, and the ability to undergo epithelial-mesenchymal transition (eMT), were significantly inhibited following the transfection of short hairpin RNA (shRNA)-FEZF1-AS1 into Y79 cells. Further experimental validation confirmed that miR-1236-3p may be a direct target of FEZF1-AS1. Notably, the miR-1236-3p inhibitor was discovered to reverse the inhibitory effects of shRNA-FEZF1-AS1 on cell viability, invasion, migration and EMT. In conclusion, the findings of the present study suggested that lncRNA-FEZF1-AS1 may promote the viability, migration, invasion and EMT of Rb cells by modulating miR-1236-3p.

Comprehensive Analysis of Serum Small Extracellular Vesicles-Derived Coding and Non-Coding RNAs from Retinoblastoma Patients for Identifying Regulatory Interactions

“…Highly aberrant lncRNAs detected in our RB sEVs OXCT1-AS1, HPYR1, HDAC2-AS2, LINC02499, slc8a1-as1, ZFAND2A-DT, LINC01359, SCOC-AS1 have not yet been reported in RB. Except AFAP1-AS1 and BANCR, all other RB sEVs lncRNA (BDNF-AS, MALAT1, HOTAIR, PANDAR, XIST, DANCR, UCA1, ZFPM2-AS1, SNHG16, and NEAT1) expression patterns from the present study were not replicating with RB tissues [ 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 ] ( Table S10 ). The distinct RNA expression profiles in sEVs and corresponding tumor tissues, among different RB patients, and from different cohorts, could be due to intra and inter-individual variability as well as tumor heterogeneity.…”

Bioinformatic analysis identifies potential key genes in the pathogenesis of age-related macular degeneration

“…The results of the present study demonstrated that the expression levels of FEZF1 may promote the cell viability, invasion, migration, and epithelial-mesenchymal transition (EMT) of cells. [ 23 ] EMT plays a key role in the pathogenesis of subretinal fibrosis in late AMD. [ 24 ] Olfml3, another URG may be a proangiogenic factor that promotes angiogenesis.…”

RUNX1, FUS, and ELAVL1-induced circPTPN22 promote gastric cancer cell proliferation, migration, and invasion through miR-6788-5p/PAK1 axis-mediated autophagy

“…Currently, exploration of the mechanism of circRNAs is mainly focused on three aspects: “miRNA sponge,” “RBP scaffolding,” and “serving as a simple polypeptide translation template.” Of these, acting as miRNA sponges in cancer cells is considered the most prevalent mechanism. It is well-established that in many cancers, including GC, miRNAs act as oncogenes or tumor suppressors, playing a pivotal role in tumor progression [ 41 , 42 ]. As new members of the competitive endogenous RNA family and regulators of miRNA activity, circRNAs may exert a significant role in gene expression regulation in cancer and other diseases [ 43 , 44 ].…”