LncRNA MALAT1 promotes decidualization of endometrial stromal cells via sponging miR‐498‐3p and targeting histone deacetylase 4

Abstract

Decidualization of human endometrial stromal cells (hESCs) is important for the maintenance of a successful pregnancy. Histone deacetylase 4 (HDAC4) was reported to be involved in the dysfunction of decidua-derived mesenchymal stem cells.However, the role of HDAC4 underlying decidualization of hESCs remains unclear.We intended to explore the function and molecular mechanism of HDAC4 in hESCs.In vitro expansion of hESCs using a serum-free medium was used to confirm the characteristics of hESCs. Gene expression in hESCs was evaluated by reverse transcription-quantitative polymerase chain reaction. CCK-8 assay, TUNEL staining, flow cytometry analysis, and Western blot analysis were performed to test the effects of HDAC4 and metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on hESCs. RNA pull-down and luciferase reporter assays were performed to validate the relationship between genes. In this study, the characteristics of hESCs were sustained in serum-free medium during a process of propagation. HDAC4 knockdown suppressed hESCs viability and promoted hESCs apoptosis. HDAC4 was targeted by miR-498-3p in hESCs. MALAT1 bound with miR-498-3p in hESCs. HDAC4 expression was positively regulated by MALAT1 and negatively regulated by miR-498-3p in hESCs. HDAC4 upregulation countervailed the effects of MALAT1 silencing on hESCs proliferation, apoptosis, and decidualization of hESCs. Overall, MALAT1 facilitated the decidualization of hESCs via binding with miR-498-3p and upregulating HDAC4, which might provide a new direction for the maintenance of a successful pregnancy.

β‐Lactoglobulin affects the oxidative status and viability of equine endometrial progenitor cells via lncRNA‐mRNA‐miRNA regulatory associations

“…Obtained results agree with the study by Jimenez et al, who showed that inhibition of miR‐200 expression during decidualization resulted in a decreased expression of IGFBP1 and PRL 45 . Furthermore, MALAT‐1 was recognized as a molecule facilitating the decidualization of human endometrial stem cells (hESCs) via binding miR‐498‐3p and thus upregulating its target, that is histone deacetylase 4 (HDAC4) 16 . MALAT1 also regulates the viability of hESCs.…”

“…decidualization of human endometrial stem cells (hESCs) via binding miR-498-3p and thus upregulating its target, that is histone deacetylase 4 (HDAC4). 16 MALAT1 also regulates the viability of hESCs. Its downregulation promoted apoptosis associated with increased BAX/ BCL-2 ratio and suppressed the growth of hESCs.…”

“…In addition, Yu et al showed that overexpression of miR‐375 during decidualization of endometrial stromal cells in vitro significantly reduced the expression of PRL, IGFBP1 and another decidualization marker, namely forkhead box protein O1 (FOXO1) 10 . Furthermore, metastasis‐associated lung adenocarcinoma transcript 1 (MALAT1), a long non‐coding RNA, was found to improve the decidualization of ESCs via binding with miR‐498‐3p, which resulted in the upregulation of histone deacetylase 4 (HDAC4) and maintained cells viability 16 . However, Liang et al showed that MALAT‐1 increases during endometriosis and might promote the proliferation and migration of endometriotic stromal cells in a miR‐200c‐dependent manner 17 .…”

“…10 Furthermore, metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), a long non-coding RNA, was found to improve the decidualization of ESCs via binding with miR-498-3p, which resulted in the upregulation of histone deacetylase 4 (HDAC4) and maintained cells viability. 16 However, Liang et al…”

Identification and validation of immune cells and hub genes alterations in recurrent implantation failure: A GEO data mining study

“…In addition, we also predicted 35 lncRNAs associated with hub genes, many of which have been shown to play a critical role in the pregnancy process. For example, Shi et al confirmed that LncRNA MALAT1 promotes decidualization of human endometrial stromal cells (hESCs) to maintain a successful pregnancy ( Shi et al, 2022 ), and downregulated MALAT1 relates to RPL ( Wang et al, 2018 ). This study showed that MALAT1 interacted with the key gene SLC7A7 through miR-205-5p, miR-22-3p, and similarly, MALAT1 also interacts with the key gene PTGS2 through miR-1297, miR-26a-5p, miR-26b-5p, miR-28-5p, miR-3145-3p, miR-508–3.…”