LncRNA OIP5-AS1 Signatures as a Biomarker of Gestational Diabetes Mellitus and a Regulator on Trophoblast Cells

Abstract

<b><i>Objectives:</i></b> Gestational diabetes mellitus (GDM) is a common disorder in pregnant women. Long noncoding RNA (lncRNA) is a fundamental mediator in the pathogenesis of GDM. The study aimed to detect the clinical importance of lncRNA OIP5-AS1 and its underlying regulation on trophoblast cells. <b><i>Design:</i></b> The expression of OIP5-AS1 and miR-137-3p was assessed by the quantitative real-time PCR technique. The prognostic effect of OIP5-AS1 was analyzed by the receiver operating characteristic curve. The influences of OIP5-AS1 on cells were indicated by cell counting kit-8, transwell experiments, and flow cytometry. Luciferase activity assay was used to identify the target relationships among OIP5-AS1, miR-137-3p, and EZH2. <b><i>Participants:</i></b> A total of 75 pregnant women with GDM who were treated in the Dongying People’s Hospital were selected as the GDM group. Besides, 72 pregnant women with non-GDM who underwent physical examination in the same hospital were selected as the control group. <b><i>Results:</i></b> Decreased expression of OIP5-AS1 was confirmed in GDM patients, and the level of OIP5-AS1 could be used as a basis for evaluating GDM patients. Upregulation of OIP5-AS1 ameliorated the viability, migration, invasion, and apoptosis of HG-stimulated HTR-8/SVneo cells by sponging miR-137-3p. EZH2 was a direct target of miR-137-3p. <b><i>Conclusions:</i></b> OIP5-AS1 level decreased in women with GDM. OIP5-AS1 appeared to help separating GDM patients from healthy pregnant women. The OIP5-AS1/miR-137-3p/EZH2 pathway could exert its function on HG-induced HTR-8/SVneo models.

Expression and diagnostic value of lncRNA HCG18 in gestational diabetes mellitus

“…Li et al [20] explored that RPL13P5 forms a co-expression chain with TSC2 gene through the PI3K-Akt signaling pathway to promote insulin resistance in GDM patients. Another report showed OIP5-AS1 as a potential biomarker of GDM and a regulator of trophoblasts [21]. As mentioned above, HCG18 can play a key regulatory role in a variety of tumors, while its predictive and diagnostic role in GDM has not been clearly reported.…”

The role of ncRNA regulatory mechanisms in diseases—case on gestational diabetes

“…In addition, decreased expression of OIP5-AS1 was demonstrated in women with GDM. Overexpression of OIP5-AS1 can ameliorate HG-induced HTR-8/SVneo cell injury in part by sponging miR-137-3p [ 50 ]. In addition to the above mechanisms, lncRNAs can interact with other RNAs to form co-expression networks and ceRNAs networks involved in GDM pathogenesis.…”

Non-coding RNAs in diabetes mellitus and diabetic cardiovascular disease

“…Similar to miRNAs, lncRNAs have been observed to be aberrantly expressed in DM. For example, lncRNA OIP5-AS1 is reduced in GDM, while HOTAIR is highly expressed, both can be used as biomarkers of GDM ( 111 , 112 ). LncRNA RPL13p5 is highly expressed in GDM patients, forming a co-expression chain with TSC2 gene through PI3K-Akt signaling pathway to promote IR ( 113 ).…”