lncRNA OIP5-AS1 targets ROCK1 to promote cell proliferation and inhibit cell apoptosis through a mechanism involving miR-143-3p in cervical cancer

Abstract

Opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is one kind of cytoplasmic long non-coding RNA (lncRNA), which has been demonstrated to play a critical function in multiple cancers. However, the detailed mechanism of OIP5-AS1 in the regulation of cervical cancer progression is still obscure. Here, we demonstrated that lncRNA OIP5-AS1 was upregulated in cervical cancer and was correlated with poor prognosis by bioinformatics studies. OIP5-AS1 depletion inhibited cell proliferation and promoted cell apoptosis in cervical cancer cells. Furthermore, we clarified that ROCK1 was the downstream effector of OIP5-AS1 and OIP5-AS1 acted as a molecular sponge of miR-143-3p. Finally, we verified that OIP5-AS1 exerted its function in the regulation of cervical cancer progression via interacting with miR-143-3p to regulate ROCK1 expression. Our study revealed novel mechanisms about how lncRNA OIP5-AS1 executed its function in cervical cancer and thus provided potential therapeutic targets for the disease.

<p>Knockdown of MIR4435-2HG Suppresses the Proliferation, Migration and Invasion of Cervical Cancer Cells via Regulating the miR-128-3p/MSI2 Axis in vitro</p>

“…11 In another study, OIP5-AS1 expression is markedly upregulated in CC cells, which is significantly associated with lymph node metastasis and tumor stage. 9 Similarly, we found that MIR4435-2HG expression was dramatically increased in CC tissues and cells. Besides MIR4435-2HG expression exhibited significant correlations with FIGO stage and lymph node metastasis.…”

“…10,11,26 Song et al have disclosed that down-regulation of OIP5-AS1 inhibits viability and promotes apoptosis in CC cells. 9 Zhu et al have disclosed that silencing of CDKN2B-AS1 suppresses metastasis and promotes apoptosis in CC cells. 27 Liu et al have demonstrated that inhibition of lncRNA GHET1 decreases the viability and migration ability of Figure 6 MIR4435-2HG knockdown inhibits the proliferation, migration and invasion of CC cells in vitro by regulating the miR-128-3p/MSI2 axis.…”

“…8 LncRNA OIP5-AS1 has been revealed to promote the proliferation and inhibit cell apoptosis in CC. 9 LncRNA PCAT6 has been reported to accelerate the progression of CC. 10 LncRNA CAR10 expression is significantly up-regulated in CC tissues, whereas overexpression of CAR10 can promote the proliferation of CC cells.…”

Inhibited Long Non-Coding RNA OIP5-AS1 Elevated microRNA-92a to Suppress Proliferation and Metastasis of Ovarian Cancer Cells by Silencing ITGA6

“…It was found that OIP5-AS1 and ITGA6 were upregulated while miR-92a was downregulated in OC tissues when compared with adjacent tissues. Consistently, Song et al have unveiled that OIP5-AS1 was highly expressed in cervical cancer tissues [9]. A previous study has suggested that miR-92a was downregulated in breast cancer cells [16], and it has been identi ed that the expression of ITGA6 was increased in cisplatin-resistant SKOV3 and cisplatin-resistant A2780 cells, and also in drug-resistant tissues in comparison to the controls [18].…”

“…OPA-interacting protein 5 antisense transcript 1 (OIP5-AS1) situated at chromosome 15q15.1 and is evolutionarily conserved in vertebrates [8]. OIP5-AS1 has been revealed to participate in the progression of cervical cancer [9] and breast cancer [10]. Nevertheless, the role of OIP5-AS1 in OC remains rarely studied.…”