LncRNA profile of glioblastoma reveals the potential role of lncRNAs in contributing to glioblastoma pathogenesis

Abstract

Abstract. Long non-coding RNAs (lncRNAs) have recently emerged as a major class of regulatory molecules involved in a broad range of biological processes and complex diseases. Our aim was to identify important lncRNAs that might play an important role in contributing to glioblastoma (GBM) pathogenesis by conducting lncRNA and mRNA profile comparison between GBM and normal brain tissue. The differentially expressed lncRNA and mRNA profiles of the tissue between GBM patient and age-matched donor without GBM diseases were analyzed using microarrays. We propose a novel model for the identification of lncRNA-mRNA targeting relationships that combine the potential targets of the differentially expressed lncRNAs with the differentially expressed mRNA abundance data. Bioinformatic analysis of the predicted target genes (gene ontology, pathway and network analysis) was performed for further research. The lncRNA microarray reveals differentially expressed lncRNAs between GBM and normal brain tissues. In the GBM group, 654 lncRNAs were upregulated and 654 were downregulated (fold change ≥4.0 or ≤0.25, P<0.01). We found 104 matched lncRNAmRNA pairs for 91 differentially expressed lncRNAs and 84 differentially expressed genes. Target gene-related pathway analysis showed significant changes in PPAR pathways in the GBM group compared with the normal brain group (P<0.05).

Unraveling the Expression Profiles of Long Noncoding RNAs in Rat Cardiac Hypertrophy and Functions of lncRNA BC088254 in Cardiac Hypertrophy Induced by Transverse Aortic Constriction

“…As in many other studies, we discovered that the expression of some lncRNAs was related to the expressions of nearby mRNAs [28]. Previous studies have shown that the lncRNA cardiac hypertrophy-related factor was able to downregulate miR-489 expression levels and regulate Myd88 expression in hypertrophy [29]. It is possible that some microRNAs exist between BC088254 and phb2, and the effect of BC088254 are related to these genes.…”

Microarray Expression Profile Analysis of Long Non-Coding RNAs in Human Gastric Cardiac Adenocarcinoma

“…Just like protein-coding genes, lncRNAs molecules may also function as tumor oncogenes or tumor suppressors through changing chromatin structure or some protein-coding genes expression level [18]. Hence, lncRNAs can be used as attractive biomarkers for tumor diagnosis and prognosis [19]. However, the roles of lncRNAs in the development of GCA and the correlation between GCA and the expression levels of these lncRNAs remains unknown.…”

Long non‐coding RNAs in brain tumours: Focus on recent epigenetic findings in glioma

“…Several studies have correlated lncRNA expression profiles with different histological subtypes and malignancy grades in gliomas 32, 36, 37. Global gene expression analyses identified 27 lncRNAs that are differentially expressed between astrocytomas and oligodendrogliomas 32.…”