LncRNA RUSC1-AS1 promotes osteosarcoma progression through regulating the miR-340-5p and PI3K/AKT pathway

Tong, Chang-Jun; Deng, Qingchun; Ou, Dijun; Long, Xia; He, Liu; Huang, Kang

doi:10.18632/aging.203047

Cited by 21 publications

(12 citation statements)

References 42 publications

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“…For instance, UNC5B-AS1 promotes the malignant progression of hepatocellular carcinoma, ovarian cancer, prostate cancer, lung cancer, and thyroid cancer ( 36 – 40 ). Notably, the tumor promotion role of RUSC1-AS1 has been well-characterized in OS ( 41 , 42 ). Rui Jiang et al.…”

Section: Discussionmentioning

confidence: 99%

Identification of cuproptosis-related lncRNA prognostic signature for osteosarcoma

Liu

Feng

et al. 2022

Front. Endocrinol.

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BackgroundCopper is an indispensably mineral element involved in various metabolic processes and functions in the active sites of many metalloproteins. Copper dysregulation is associated with cancers such as osteosarcoma (OS), the most common primary bone malignancy with invasiveness and metastasis. However, the causality between cuproptosis and OS remains elusive. We aim to identify cuproptosis-related long non-coding RNAs (lncRNAs) for osteosarcomatous prognosis, immune microenvironment response, and immunotherapy.MethodsThe Person correlation and differential expression analysis were used to identify differentially expressed cuproptosis-related lncRNAs (CRLs). The univariate, least absolute shrinkage and selection operator (LASSO), and multivariate Cox regression analysis were performed to construct the CRL signature. The Kaplan–Meier (K-M) survival analysis, receiver operating characteristic (ROC) curve, internal validation, independent prognostic analysis, and nomograph were used to evaluate the prognostic value. The functional enrichment, tumor microenvironment, immunotherapy and chemotherapy response between the two distinct groups were further explored using a series of algorithms. The expression of signature CRLs was verified by real-time quantitative polymerase chain reaction (RT-qPCR) in OS cell lines.ResultsA novel CRL signature consisting of four CRLs were successfully identified. The K-M survival analysis indicated that the OS patients in the low-risk groups had a better prognosis than that in the high-risk group. Then, the ROC curve and subgroup survival analysis confirmed the prognostic evaluation performance of the signature. Equally, the independent prognostic analysis demonstrated that the CRL signature was an independently predicted factor for OS. Friends analysis determined the hub genes that played a critical role in differentially expressed genes between two distinct risk groups. In addition, the risk score was related to immunity status, immunotherapy response, and chemotherapeutic drug sensitivity. Finally, the expression of these signature CRLs detected by RT-qPCR was consistent with the bioinformatic analysis results.ConclusionIn summary, our study confirmed that the novel CRL signature could effectively evaluate prognosis, tumor immune microenvironment, and immunotherapy response in OS. It may benefit for clinical decision-making and provide new insights for personalized therapeutics.

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Section: Discussionmentioning

confidence: 99%

Identification of cuproptosis-related lncRNA prognostic signature for osteosarcoma

Liu

Feng

et al. 2022

Front. Endocrinol.

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“…It was also found to be upregulated in osteosarcoma in the same study as COX10-AS1, which is mentioned above [ 95 ]. There are many more studies exploring its interactions with miRNAs in tumor progression [ 97 , 98 ]. In the present systematic review, it was found to be upregulated in patients with Sertoli cell-only syndrome [ 29 ], and deregulated in asthenozoospermia [ 41 ] and oligozoospermia [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

The Role of Long Noncoding RNAs on Male Infertility: A Systematic Review and In Silico Analysis

Kyrgiafini

Sarafidou

Mamuris

2022

Biology

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Male infertility is a complex disorder affecting many couples worldwide. Long noncoding RNAs (lncRNAs) regulate important cellular processes; however, a comprehensive understanding of their role in male infertility is limited. This systematic review investigates the differential expressions of lncRNAs in male infertility or variations in lncRNA regions associated with it. The PRISMA guidelines were used to search Pubmed and Web of Science (1 June 2022). Inclusion criteria were human participants, patients diagnosed with male infertility, and English language speakers. We also performed an in silico analysis investigating lncRNAs that are reported in many subtypes of male infertility. A total of 625 articles were found, and after the screening and eligibility stages, 20 studies were included in the final sample. Many lncRNAs are deregulated in male infertility, and interactions between lncRNAs and miRNAs play an important role. However, there is a knowledge gap regarding the impact of variants found in lncRNA regions. Furthermore, eight lncRNAs were identified as differentially expressed in many subtypes of male infertility. After in silico analysis, gene ontology (GO) and KEGG enrichment analysis of the genes targeted by them revealed their association with bladder and prostate cancer. However, pathways involved in general in tumorigenesis and cancer development of all types, such as p53 pathways, apoptosis, and cell death, were also enriched, indicating a link between cancer and male infertility. This evidence, however, is preliminary. Future research is needed to explore the exact mechanism of action of the identified lncRNAs and investigate the association between male infertility and cancer.

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“…Furthermore, rno-mir-340-5p and rno-mir-21-5p presented many target genes enriched in the PI3K-Akt signaling pathway, which is closely related to corneal transplantation rejection. Additionally, rno-mir-340-5p plays a role in the progression of osteosarcoma through the PI3K Akt pathway [20]. However, the mechanisms of rno-mir-340-5p/PI3K-Akt have been rarely reported.…”

Section: Discussionmentioning

confidence: 99%

Expression and Predictive Functional Profiles of MicroRNAs Data in Vascularized Composite Allotransplantation Acute Rejection

Fang¹,

Li²,

Zhu³

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: This study aimed to investigate the mechanisms of acute rejection for vascularized composite allotransplantation (VCA) using microRNAs (miRNAs) differential expression in a VCA animal model. Methods: Brown Norway rats were used as transplant donors and Lewis rats as VCA receptors. The changes were divided into different stages before and after transplantation in Lewis rats, and all appearance changes were recorded. Also, histological evaluations were performed on all recipients, and the expression of microRNAs was analyzed when acute immune rejection occurred. Then, we used GO and KEGG Pathway enrichment analyses to predict miRNA targets. Finally, differentially expressed miRNAs were detected by RT-qPCR. Results: Compared to pre-operation, 22 miRNAs were differentially expressed after operations. Among them, nine were upregulated, and 13 were downregulated in skin tissues. The RT-qPCR results revealed that rno-miR-340-5p and rno-miR-21-5p were significantly upregulated and enriched in the PI3K-Akt signaling pathway. Moreover, rno-miR-145-5p and rno-miR-195-5p were significantly downregulated, and most of their target genes were enriched in the Hippo signaling pathway. The histological evaluations showed that, after VCA, the skin tissue presented severe acute rejection. Conclusions: The miRNAs rno-miR-340-5p, rno-miR-21-5p, rno-miR-145-5p, and rno-miR-195-5p were significantly regulated during VCA acute rejection, when the four miRNAs analyses were done on skin biopsies. These miRNAs might be potential biomarkers for objective, early, and minimally invasive rejection diagnosis.

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LncRNA RUSC1-AS1 promotes osteosarcoma progression through regulating the miR-340-5p and PI3K/AKT pathway

Cited by 21 publications

References 42 publications

Identification of cuproptosis-related lncRNA prognostic signature for osteosarcoma

Identification of cuproptosis-related lncRNA prognostic signature for osteosarcoma

The Role of Long Noncoding RNAs on Male Infertility: A Systematic Review and In Silico Analysis

Expression and Predictive Functional Profiles of MicroRNAs Data in Vascularized Composite Allotransplantation Acute Rejection

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