LncRNA SNHG5 promotes growth and invasion in melanoma by regulating the miR-26a-5p/TRPC3 pathway

Gao, Jun; Zeng, Kang; Liu, Yi; Gao, Lin; Liu, Lishi

doi:10.2147/ott.s184078

Cited by 57 publications

(40 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Accumulating research has confirmed the importance of lncRNAs in tumorigenesis. 17,18 In HCC, several lncRNAs were identified as important regulators. For instance, SNHG16 overexpression suppresses HCC cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

LINC00339 promotes growth and invasiveness of hepatocellular carcinoma by the miR-1182/SKA1 pathway

Xiao¹,

Yu²,

Ma³

2019

OTT

View full text Add to dashboard Cite

Background: Extensive research has shown that long noncoding RNA (lncRNA) is involved in tumorigenesis, including hepatocellular carcinoma (HCC). The lncRNA LINC00339 was reported to regulate the development of lung cancer or breast cancer. However, whether LINC00339 participates in HCC progression remains unclear. Here, our results showed that LINC00339 was upregulated in HCC. Methods: qRT-PCR and in situ hybridization (ISH) was used to analyze LINC00339 expression in tumor tissues and cell lines. CCK8 and colony formation assays were used to analyze cell proliferation. Transwell assay was used to analyze cell migration and invasion. Xenograft experiment was used to test tumor growth in vivo. Results: LINC00339 overexpression was correlated with an advanced stage, metastasis, and bad prognosis in HCC patients. Functional investigation showed that LINC00339 knockdown significantly suppressed HCC cell proliferation, migration, and invasion. Moreover, decreased LINC00339 expression inhibited HCC growth in vivo. Mechanistically, LINC00339 could interact with miR-1182 to promote SKA1 expression. We also demonstrated that SKA1 acted as an oncogene and SKA1 upregulation reversed the effect of LINC00339 silencing. Conclusion: Our results illustrated that the LINC00339/miR-1182/SKA1 axis plays an essential role in HCC progression.

show abstract

Section: Discussionmentioning

confidence: 99%

LINC00339 promotes growth and invasiveness of hepatocellular carcinoma by the miR-1182/SKA1 pathway

Xiao¹,

Yu²,

Ma³

2019

OTT

View full text Add to dashboard Cite

show abstract

“…Through miR-26 sponging and up-regulation of TRPC3 (transient receptor potential channel 3), SNHG5 stimulates melanoma cell growth. The exogenous knockdown of SNHG5 in melanoma cells in vitro decreases their proliferation rate and invasive capacity, while it stimulated apoptosis (91). Further research and details on the role of SNHG5 in malignant diseases are presented in Supplementary Table 3.…”

Section: Small Nucleolar Rna Host Gene 5 (Snhg5)mentioning

confidence: 99%

An Emerging Class of Long Non-coding RNA With Oncogenic Role Arises From the snoRNA Host Genes

et al. 2020

View full text Add to dashboard Cite

The small nucleolar RNA host genes (SNHGs) are a group of long non-coding RNAs, which are reported in many studies as being overexpressed in various cancers. With very few exceptions, the SNHGs (SNHG1, SNHG3, SNHG5, SNHG6, SNHG7, SNHG12, SNHG15, SNHG16, SNHG20) are recognized as inducing increased proliferation, cell cycle progression, invasion, and metastasis of cancer cells, which makes this class of transcripts a viable biomarker for cancer development and aggressiveness. Through our literature research, we also found that silencing of SNHGs through small interfering RNAs or short hairpin RNAs is very effective in both in vitro and in vivo experiments by lowering the aggressiveness of solid cancers. The knockdown of SNHG as a new cancer therapeutic option should be investigated more in the future.

show abstract

“…2 Due to melanoma's susceptibility to metastasis, the prognosis of melanoma patients with advanced disease is unfavorable, with five-year survival rates below 20%. 3 Therefore, clarifying the mechanisms of melanoma pathogenesis and establishing biomarkers for early diagnosis and therapy would be a significant milestone in improving patient outcomes.…”

Section: Introductionmentioning

confidence: 99%

Long Noncoding RNA X-Inactive Specific Transcript Facilitates Cellular Functions in Melanoma via miR-139-5p/ROCK1 Pathway

Tian

Sun

Chen

et al. 2020

OTT

View full text Add to dashboard Cite

Although X-inactive specific transcript (XIST) is known to play a critical role in the pathogenesis of melanoma, the mechanisms through which this remains unclear. Methods: RNAseq, immunohistochemistry, and qRT-PCR were used to identify the levels of XIST, miR-139-5p, and Rho-Associated Coiled-Coil Containing Protein Kinase-1 (ROCK1) in melanoma tissues and cells. A subcellular fractionation assay was used to determine the location of XIST. CCK-8 and colony formation assays were used to evaluate cellular proliferation. Cell migration and wound healing assays were used to detect the effects on cell migration. RNA pull-down was used to confirm the interaction between XIST and miR-139-5p. Besides, the xenograft tumor experiment was performed to further verify the roles of XIST in melanoma. Results: In this study, an increased level of XIST was revealed in melanoma tissues and cells, which was associated with higher TNM stage and positive lymph node metastasis. XIST was found to function as a "molecular sponge" of miR-139-5p to facilitate cellular functions. Moreover, these consequences could be partially reversed by inhibition of miR-139-5p. MiR-139-5p was found to target ROCK1 directly, leading to suppression of ROCK1 expression; this effect could be partially reversed by inhibiting XIST expression. Furthermore, the deletion of ROCK1 induced anti-oncogenic effects similar to those seen with knockout of XIST. Upregulation of miR-139-5p and knockdown of XIST could inhibit cell functions in melanoma. Conclusion: Our findings suggested that the lncRNA XIST facilitates cellular functions in melanoma via the miR-139-5p/ROCK1 pathway.

show abstract

LncRNA SNHG5 promotes growth and invasion in melanoma by regulating the miR-26a-5p/TRPC3 pathway

Cited by 57 publications

References 32 publications

<p>LINC00339 promotes growth and invasiveness of hepatocellular carcinoma by the miR-1182/SKA1 pathway</p>

<p>LINC00339 promotes growth and invasiveness of hepatocellular carcinoma by the miR-1182/SKA1 pathway</p>

An Emerging Class of Long Non-coding RNA With Oncogenic Role Arises From the snoRNA Host Genes

<p>Long Noncoding RNA X-Inactive Specific Transcript Facilitates Cellular Functions in Melanoma via miR-139-5p/ROCK1 Pathway</p>

Contact Info

Product

Resources

About