lncRNA TUG1 Facilitates Colorectal Cancer Stem Cell Characteristics and Chemoresistance by Enhancing GATA6 Protein Stability

Sun, Junfeng; Zhou, Hangyuan; Bao, Xingqi; Wu, Yue; Jia, Haowei; Zhao, Hongchao; Liu, Guanghui

doi:10.1155/2021/1075481

Cited by 15 publications

(8 citation statements)

References 42 publications

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“…According to related studies, LncRNA TUG1, a chromatinmodifying complex located on human chromosome 22q12.2, has a high expression in vascular endothelial cells and regulates the CP and CA of oncocytes, making it a potential tumor biomarker and therapeutic target [2]. Cur-rently, a lot of research has authenticated that LncRNA TUG1 influences the malignant behavior of tumors by inhibiting miRNAs [3,4]. For instance, LncRNA TUG1 can adsorb miR-29c-3p, which enhances the malignant behavior of gastric adenocarcinoma [5].…”

Section: Introductionmentioning

confidence: 99%

Downregulation of miR-221-3p by LncRNA TUG1 Promoting the Healing of Closed Tibial Fractures in Mice

Liu

Yuan

2022

BioMed Research International

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Objective. To probe into the effect of LncRNA TUG1 on the healing of closed tibial fracture in mice. Methods. The closed tibial fracture model of mice was established, selecting the mouse osteoblast line MC3T3-E1, with the cells separated into four groups. The expression levels of TUG1 and miR-221-3p were determined by RT-qPCR analysis, with the targeting relationship between TUG1 and miR-221-3p authenticated by dual luciferase reporter (DLR) assay, detection of cell migration (CM) ability based on Transwell cell migration (TCM) assay, and cell proliferation (CP) acquired by cell counting kit-8 (CCK-8). Results. Prediction results of the target gene by bioinformatics software showed that miR-221-3p had binding sites with the 3 ′ -UTR of TUG1, and DLR assay authenticated the targeting relationship between LncRNA TUG1 and miR-221-3p. Downregulation of TUG1 inhibited osteoblast CP and CM and promoted osteoblast cell apoptosis (CA). Cell cycle analysis indicated that miR-221-3p provoked cell cycle arrest in G1 stage of MC3T3-E1 cells. The siLncRNA-NC group had higher anticyclin D1 and D3 levels than the siLncRNA TUG1 group, with a lower CA rate in the former, implying that miR-221-3p overexpression inhibited osteoblast CP and CM and LncRNA TUG1 inhibited CA. Downregulation of miR-221-3p partly reversed the retardation out of downregulating TUG1 on osteoblast CP and CM. Bcl-2 level was higher in the LncRNA TUG1 group compared to the siLncRNA TUG1 and miR-221-3p overexpression groups, with remarkably lower SDF-1 level in the miR-221-3p overexpression group than those in the control, miRNA-NC, and LncRNA TUG1 groups. Conclusion. The downregulation of miR-221-3p by LncRNA TUG1 can promote the healing of closed tibial fractures in mice.

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Section: Introductionmentioning

confidence: 99%

Downregulation of miR-221-3p by LncRNA TUG1 Promoting the Healing of Closed Tibial Fractures in Mice

Liu

Yuan

2022

BioMed Research International

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“…Sun et al. discovered that TUG1 increased chemoresistance and enhanced cancer stem cell behaviors via stabilizing GATA6 protein in colorectal cancer ( 68 ). TUG1 targeted AKT/mTOR signaling pathway via sponging miR-582-3p, which promoted ovarian cancer malignant behaviors ( 69 ).…”

Section: Lncrnas Regulate the Expression Of F-box Proteinsmentioning

confidence: 99%

The functions of long noncoding RNAs on regulation of F-box proteins in tumorigenesis and progression

et al. 2022

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Accumulated evidence has revealed that F-box protein, a subunit of SCF E3 ubiquitin ligase complexes, participates in carcinogenesis and tumor progression via targeting its substrates for ubiquitination and degradation. F-box proteins could be regulated by cellular signaling pathways and noncoding RNAs in tumorigenesis. Long noncoding RNA (lncRNA), one type of noncoding RNAs, has been identified to modulate the expression of F-box proteins and contribute to oncogenesis. In this review, we summarize the role and mechanisms of multiple lncRNAs in regulating F-box proteins in tumorigenesis, including lncRNAs SLC7A11-AS1, MT1JP, TUG1, FER1L4, TTN-AS1, CASC2, MALAT1, TINCR, PCGEM1, linc01436, linc00494, GATA6-AS1, and ODIR1. Moreover, we discuss that targeting these lncRNAs could be helpful for treating cancer via modulating F-box protein expression. We hope our review can stimulate the research on exploration of molecular insight into how F-box proteins are governed in carcinogenesis. Therefore, modulation of lncRNAs is a potential therapeutic strategy for cancer therapy via regulation of F-box proteins.

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“…Recent studies prove that specific ncRNAs may be the key targets in overcoming chemotherapeutic resistance. In case of the lncRNA subgroup in CRC these are for example HOTAIRM1 [ 30 ], lncRNA-cCSC1 [ 26 ], SCARNA2 [ 31 ], LINC01347 [ 32 ], HAND2-AS1 [ 33 ], lnc273–31, and lnc273–34 [ 34 ], TUG1 [ 35 ].…”

Section: Chemoresistance-associated Ncrnasmentioning

confidence: 99%

“…TUG1 is another lncRNA inducing oxaliplatin resistance in CRC stem cells, where its expression is elevated. It is proven to stabilise transcription factor GATA6 [ 35 ].…”

Section: Chemoresistance-associated Ncrnasmentioning

confidence: 99%

Long Non-Coding RNA and microRNA Interplay in Colorectal Cancer and Their Effect on the Tumor Microenvironment

Rajtmajerová

Trailin

Liška

et al. 2022

Cancers

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As the current staging and grading systems are not sufficient to stratify patients for therapy and predict the outcome of the disease, there is an urgent need to understand cancer in its complexity. The mutual relationship between tumour and immune or stromal cells leads to rapid evolution and subsequent genetic and epigenetic changes. Immunoscore has been introduced as a diagnostic tool for colorectal cancer (CRC) only recently, emphasising the role of the specific tumor microenvironment in patient’s prognosis and overall outcome. Despite the fact that non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), cannot be translated into proteins, they significantly affect cell’s transcriptome and translatome. miRNA binding to mRNA efficiently blocks its translation and leads to mRNA destruction. On the other hand, miRNAs can be bound by lncRNAs or circular RNAs (circRNAs), which prevents them from interfering with translation. In this way, ncRNAs create a multi-step network that regulates the cell’s translatome. ncRNAs are also shed by the cell as exogenous RNAs and they are also found in exosomes, suggesting their role in intercellular communication. Hence, these mechanisms affect the tumor microenvironment as much as protein signal molecules. In this review, we provide an insight into the current knowledge of the microenvironment, lncRNAs’, and miRNAs’ interplay. Understanding mechanisms that underlie the evolution of a tissue as complex as a tumour is crucial for the future success in therapy.

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lncRNA TUG1 Facilitates Colorectal Cancer Stem Cell Characteristics and Chemoresistance by Enhancing GATA6 Protein Stability

Cited by 15 publications

References 42 publications

Downregulation of miR-221-3p by LncRNA TUG1 Promoting the Healing of Closed Tibial Fractures in Mice

Downregulation of miR-221-3p by LncRNA TUG1 Promoting the Healing of Closed Tibial Fractures in Mice

The functions of long noncoding RNAs on regulation of F-box proteins in tumorigenesis and progression

Long Non-Coding RNA and microRNA Interplay in Colorectal Cancer and Their Effect on the Tumor Microenvironment

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