LncRNA TUG1 influences papillary thyroid cancer cell proliferation, migration and EMT formation through targeting miR-145

Abstract

LncRNA TUG1, a tumor oncogene associated with various human cancers, has been reported to be involved in regulating various cellular processes, such as proliferation, apoptosis and invasion through targeting multiple genes. However, its biological function in thyroid cancer cells has not been elucidated. The aim of this study is to measure TUG1 expression level and evaluate its function in thyroid cancer cells. LncRNA TUG1 expression levels in thyroid cancer tissues and three thyroid cancer cell lines (the ATC cell lines SW1736 and KAT18 and the FTC cell line FTC133) were assessed by qRT-PCR and compared with that of the human normal breast epithelial cell HGC-27. MTT assay, colony formation assay, transwell assay and western blot analysis were performed to assess the effects of TUG1 on proliferation, metastasis and EMT formation in thyroid cancer cells in vitro. Rescue assay was performed to further confirm that TUG1 contributes to the progression of thyroid cancer cells through regulating miR-145/ZEB1 signal pathway. LncRNA TUG1 was found to be up-regulated in thyroid cancer tissues and thyroid cancer cells compared with that in the human normal breast epithelial cell HGC-27. Increased lncRNA TUG1 expression was found to significantly promote tumor cell proliferation, and facilitate cell invasion, while down-regulated TUG1 could obviously inhibit cell proliferation, migration/invasion and reverse EMT to MET. These results indicated that TUG1 may contribute to the progression of thyroid cancer cells by function as a ceRNA competitive sponging miR-145, and that lncRNA TUG1 is associated with tumor progression in thyroid cancer cells.

Retracted: LINC00311 promotes cancer stem‐like properties by targeting miR‐330‐5p/TLR4 pathway in human papillary thyroid cancer

“…For instance, lncRNA TUG1 contributes to the progression of PTC by regulating miR‐145 . Another lncRNA called ABHD11‐AS1 was dramatically upregulated in PTC cells and promoted tumorigenesis via modulating BHD11‐AS1/miR‐199a‐5p/SLC1A5 axis . In addition, lncRNA myocardial infarction‐associated transcript (MIAT) was also highly expressed in PTC cells and enhanced tumor progression .…”

Long noncoding RNA NEAT1 regulate papillary thyroid cancer progression by modulating miR‐129‐5p/KLK7 expression

“…As well as in the research of Liu et al () which displays a correlation between lncRNA H19, miR‐17‐5p, and YES1 in thyroid cancer as well as in Li, Shen, Li, Zhang, and Jin ()'s research which revealed the correlation between lncRNA n340790 and miR‐1254 in carcinogenesis acceleration of thyroid cancer. LncRNA/miRNA has been found to related with Wnt/β‐Catenin signaling (Wang, Lu, Geng, Yang, & Shi, ) or EMT formation (Lei, Gao, & Xu, ) in thyroid cancer. Their effectiveness in the treatment of PTC could be compared and applied into clinical researches in the hope of better the prognosis.…”

LncRNA TNRC6C‐AS1 regulates UNC5B in thyroid cancer to influence cell proliferation, migration, and invasion as a competing endogenous RNA of miR‐129‐5p

“…H19 was reported to competitively bind to miR‐17‐5p to regulate YES1 expression in TC, resulting in the promotion of TC cell proliferation and invasion . TUG1 also contributed to the progression of TC by functioning as a ceRNA competitively sponging miR‐145 . Similarly, through sponging miR‐129‐5p, TNRC6C‐AS1 indirectly upregulated UNC5B expression, thus promoting TC development.…”