LncRNAs are regulated by chromatin states and affect the skeletal muscle cell differentiation

Qi, Xiaolong; Hu, Mingyang; Xiang, Yue; Wang, Daoyuan; Xu, Yongming; Hou, Ye; Zhou, Huanhuan; Luan, Yu; Wang, Zhangxu; Zhang, Weiya; Li, Xinyun; Song, Zhao; Zhao, Yi‐Fang

doi:10.1111/cpr.12879

Cited by 13 publications

(9 citation statements)

References 57 publications

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“…Some lncRNAs play functional roles in myoblast proliferation and differentiation, such as lnc-ORA [ 49 ], IGF2 AS [ 50 ], SMUL [ 51 ], and lncIRS1 [ 52 ]. In this study, we found that the expression of Gm10561 was upregulated during C2C12 differentiation, consistent with a previous report [ 41 ]. Knockdown of Gm10561 remarkably suppressed proliferation and differentiation of C2C12 myoblasts, while Gm10561 overexpression had the opposite effect.…”

Section: Discussionsupporting

confidence: 93%

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Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432

et al. 2022

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Skeletal myogenesis is a highly ordered process finely regulated by various factors. Long non-coding RNAs play an important regulatory role in myogenesis via multiple mechanisms. In this study, we identified the lncRNA Gm10561 , which was upregulated during myogenic differentiation and is highly expressed in skeletal muscle. Knockdown of Gm10561 inhibited the proliferation and differentiation of C2C12 myoblasts in vitro and muscle growth in vivo . Overexpression of Gm10561 promoted the proliferation and differentiation of both C2C12 myoblasts and porcine muscle satellite cells. Notably, lncRNA Gm10561 is localized in the cytoplasm and competitively bound to miR-432, which directly targets MEF2C and E2F3 . It was confirmed that lncRNA Gm10561 regulates the proliferation and differentiation of myoblasts by acting as a sponge of miR-432 to modulate MEF2C and E2F3 expression. Thus, the lncRNA- Gm10561 -miR-432- MEF2C / E2F3 axis plays an important role in myogenesis.

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Section: Discussionsupporting

confidence: 93%

“…A recent study identified 385 differential expressed lncRNAs during C2C12 differentiation, whose expression were controlled by changes of chromatin state, using RNA-seq combined with ATAC-seq and ChIP-seq analysis. G m10561 was one of the differentially expressed lncRNAs [ 41 ]. However, the role of Gm10561 in myogenesis is unknown.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432

et al. 2022

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“…Notably, the foetal stage is the critical period of myogenesis, when the majority of skeletal muscle fibres form 5 . It has become established that many specific signalling pathways and transcription factors (TFs) are involved in myogenesis 5–8 . Wingless and Int (Wnt), paired box 3 ( Pax3 ), and Pax7 are necessary myogenic regulatory factors ( MRFs ) that regulate the myogenic lineage differentiation of mesenchymal stem cells during embryo stage 5,9–12 .…”

Section: Introductionmentioning

confidence: 99%

“…5 It has become established that many specific signalling pathways and transcription factors (TFs) are involved in myogenesis. [5][6][7][8] Wingless and Int (Wnt), paired box 3 (Pax3), and Pax7 are necessary myogenic regulatory factors (MRFs) that regulate the myogenic lineage differentiation of mesenchymal stem cells during embryo stage. 5,[9][10][11][12] Although previous studies provide strong evidence of the contribution by MRFs to skeletal muscle development, 13,14 the precise mechanisms underlying skeletal muscle development remain unclear.…”

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confidence: 99%

Transcriptional states and chromatin accessibility during bovine myoblasts proliferation and myogenic differentiation

Wang

et al. 2022

Cell Proliferation

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Objectives: Although major advances have been made in bovine epigenome study, the epigenetic basis for fetal skeletal muscle development still remains poorly understood. The aim is to recapitulated the time course of fetal skeletal muscle development in vitro, and explore the dynamic changes of chromatin accessibility and gene expression during bovine myoblasts proliferation and differentiation.Methods: PDGFR-cells were isolated from bovine fetal skeletal muscle, then cultured and induced myogenic differentiation in vitro in a time-course study (P, D0, D2, and D4). The assay for transposase-accessible chromatin sequencing (ATAC-seq) and RNA sequencing (RNA-seq) were performed.Results: Among the enriched transcriptional factors with high variability, we determined the effects of MAFF, ZNF384, and KLF6 in myogenesis using RNA interference (RNAi). In addition, we identified both stage-specific genes and chromatin accessibility regions to reveal the sequential order of gene expression, transcriptional regulatory, and signal pathways involved in bovine skeletal muscle development. Further investigation integrating chromatin accessibility and transcriptome data was conducted to explore cis-regulatory regions in line with gene expression. Moreover, we combined bovine GWAS results of growth traits with regulatory regions defined by chromatin accessibility, providing a suggestive means for a more precise annotation of genetic variants of bovine growth traits. Conclusion:Overall, these findings provide valuable information for understanding the stepwise regulatory mechanisms in skeletal muscle development and conducting beef cattle genetic improvement programs. | INTRODUCTIONSkeletal muscle, which accounts for about 40% of total body weight, 1 is a highly dynamic tissue of the body. The growth and maintenance of skeletal muscle play an important role in crucial Qian Li and Yahui Wang contributed equally to this work and shared the first authorship.

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“…Current evidence suggests that lncRNAs may act as molecular sponges of miRNAs via their miRNA response elements (MREs) for the regulation of miRNA expression, thereby depressing the speci c target genes of the miRNAs, which is described as the competing endogenous RNA (ceRNA) hypothesis. lncRNAs have been demonstrated to play crucial roles in various biological processes, such as in ammation, proliferation, differentiation, immune escape, migration and apoptosis [27][28][29][30][31]. Accumulating evidence suggests that this regulatory action plays pivotal roles during the development of ischemic stroke [32][33][34].…”

Section: Introductionmentioning

confidence: 99%

miR-212-5p protects the brain against ischemic damage in rats and its function is regulated by the lncfos/miR-212-5p/CASP7 axis

Qiu

Qin

et al. 2022

Preprint

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miR-212-5p has been reported to be involved in many biological processes. However, the role of miR-212-5p in ischemic stroke remains unclear. This study aimed to explore the biological role and potential mechanism of miR-212-5p inbrain damage in rats with ischemic stroke by investigating the lncfos/miR-212-5p/CASP7 axis. Rats were subjected to middle cerebral artery occlusion (MCAO) and intracerebroventricular injection of miRNA agomir, miRNA antagomir, shRNA lentiviral vector or negative control. The neurological deficit score, infarct volume and histopathology, neuronal apoptosis, lncfos, miR-212-5p and CASP7 expression in the peri-infarct area were assessed. In this study, we found thatthe expression level of miR-212-5p was significantly downregulated in the peri-infarct area and blood in MCAO rats and in the blood of patients with ischemic stroke. The double luciferase experiment showed that CASP7 was the direct target gene of miR-212-5p and that lncfos was the direct target gene of miR-212-5p. Lateral ventricular injection of miR-212-5p agomir can effectively inhibit apoptosis induced by ischemic brain damage, reduce infarct volume, improve neurological deficit symptoms and downregulate the expression of CASP7 in the peri-infarct area in MCAO rats. Suppressing lncfos with sh-fos can upregulate the expression of miR-212-5p and plays a neuroprotective role in rat MCAO models. We conclude that miR-212-5p plays a neuroprotective role in rat MCAO models and that its function is regulated by the lncfos/miR-212-5p/CASP7 axis.

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LncRNAs are regulated by chromatin states and affect the skeletal muscle cell differentiation

Cited by 13 publications

References 57 publications

Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432

Long non-coding RNA Gm10561 promotes myogenesis by sponging miR-432

Transcriptional states and chromatin accessibility during bovine myoblasts proliferation and myogenic differentiation

miR-212-5p protects the brain against ischemic damage in rats and its function is regulated by the lncfos/miR-212-5p/CASP7 axis

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