LncRNAs in neuropsychiatric disorders and computational insights for their prediction

Baruah, Cinmoyee; Nath, Prangan; Barah, Pankaj

doi:10.1007/s11033-022-07819-x

Cited by 11 publications

(1 citation statement)

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“…LncRNAs are defined as RNA transcripts of greater than 200 nucleotides without evident protein‐coding function. LncRNAs have been observed to play versatile roles in regulating diverse biological processes including gene regulation, transcription, mRNA splicing, protein interaction, and genomic imprinting (Baruah et al, 2022; Batista & Chang, 2013). LncRNAs can interact with their targets by RNA–RNA pairings, RNA–DNA hybrids, RNA structure‐mediated interactions, and protein linkers (Ma et al, 2013; Wilusz et al, 2009; Xu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

LncRNA CARMN facilitates odontogenic differentiation of dental pulp cells by impairing EZH2

Li,

Huo,

et al. 2023

Oral Diseases

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ObjectiveThis study aimed to reveal the potential role of CARMN in odontogenic differentiation of dental pulp cells (DPCs).MethodsLaser capture microdissection was used to detect Carmn in DPCs and odontoblasts in P0 mice. After manipulating CARMN expression in odontogenic differentiation induced hDPCs, the state of odontogenic differentiation was evaluated by ALP staining, ARS, and related marker expression in qRT‐PCR and western blotting. The subcutaneous transplantation of HA/β‐TCP loaded with hDPCs was performed to verify CARMN's role in promoting odontogenic differentiation in vivo. RNAplex and RIP were employed to reveal potential mechanism of CARMN in hDPCs.ResultsCARMN expressed more abundantly in odontoblasts than DPCs in P0 mice. CARMN expression boosted during in vitro odontogenic differentiation of hDPCs. CARMN overexpression enhanced odontogenic differentiation of hDPCs in vitro, while inhibition impaired the process. CARMN overexpression in HA/β‐TCP composites promoted more mineralized nodule formation in vivo. CARMN knockdown led to soared EZH2, while CARMN overexpression brought about EZH2 inhibition. CARMN functioned via direct interaction with EZH2.ConclusionsThe results uncovered CARMN as a modulator during the odontogenic differentiation of DPCs. CARMN promoted odontogenic differentiation of DPCs by impairing EZH2.

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