Loach embryos prooxidant-antioxidant status under the influence of amide derivatives of 1,4-naphthoquinone

Abstract

The mechanisms of disorders in cell functions induced by 1,4-naphthoquinone amide derivatives are not clarified yet. The article is dedicated to the study of features of these substances influence on loach Misgurnus fossilis L. embryos pro/antioxidant homeostasis during early embryogenesis. The aim of this work was to study the effect of 2-chloro-3-hydroxy-1,4-naphthoquinone, 2-chloro-3-(3-oxo-3-(piperidine-1-yl)propylamine)-1,4-naphthoquinone (FO-1), 2-chloro-3-(3-(morpholine-4-yl)-3-oxopropylamine)-1,4-naphthoquinone (FO-2 at concentrations of 10-3, 10-5, 10-7 M on the content of TBA-reactive substances (a byproduct of lipid peroxidation) and the activities of superoxide dismutase and catalase in loach embryos. It was established that 1,4-naphthoquinone amide derivatives and 2-chloro-3-hydroxy-1,4-naphthoquinone decreased the content of lipid peroxidation products in embryo cells in a dose-dependent manner. The investigated compounds cause an increase in superoxide dismutase and catalase activities compared with the control value. The results of the two-factor ANOVA test indicate that 2-chloro-3-hydroxy-1,4-naphthoquinone and 1,4-naphthoquinone amide derivatives (FO-1, FO-2) have predominant influence on the TBA-reactive substances content and superoxide dismutase activity. However, the time of loach embryos development has a more pronounced effect on catalase activity than the studied 1,4-naphthoquinone derivatives.

Two novel 1,4‑naphthoquinone derivatives induce human gastric cancer cell apoptosis and cell cycle arrest by regulating reactive oxygen species‑mediated MAPK/Akt/STAT3 signaling pathways

“…1,4-Naphthoquinones are active quinone derivatives that exhibit several different biological responses including anti-inflammatory, anticancer, apoptosis and radical scavenging activities (21); however, the severe side effects have limited their utility as clinical agents. To reduce their side effects and improve the effects of 1,4-naphthoquinone derivatives, BQ and OQ were synthesized and their potential mechanisms were explored in human gastric cancer cells.…”

Ультраструктура Зародків В’юна За Впливу Новосинтезованих Амідних Похідних 1,4-Нафтохінону

“…Дослідженнями підтверджено [8], що 2-гідрокси-3-хлоро-1,4-нафтохінон (Mr=211) й амідні похідні ФО-1, ФО-2 у концентраціях 10 -3 , 10 -5 , 10 -7 М сприяють зниженню вмісту вторинних продуктів пероксидного окиснення ліпідів (ТБК-активних продуктів) у зародках в'юна. За їхньої дії зростає активність супероксиддисмутази та каталази, порівняно з контролем.…”