This paper provides closed-loop tuning of the cascaded-tilted integral derivative controller (CC-TID) for load frequency control (LFC) of a microgrid system. A microgrid system is the arrangement of distributed generation resources such as a wind turbine generator (WTG), fuel cell (FC), aqua electrolyzer (AE), diesel engine generator (DEG), and battery energy storage system (BESS). Different controllers such as proportional integral derivative (PID), two degrees of freedom (2DOFPID), three degrees of freedom (3DOFPID), and tilted integral derivative (TID) are used not only to sustain the disparity between real power generation and load demand but also accomplish zero steady-state error to enrich the frequency and tie power regulations. The anticipated controller encompasses both the value of cascade (CC) and fractional order (FO) controls for better elimination of system instabilities. In the proposed CC-3DOFPID-TID controller, the TID controller is cast-off as a slave controller, and the 3DOFPID controller aided the role of the dominant controller. The controlled parameters are optimized by an adaptive symbiotic organism search (ASOS) algorithm for keen results of difficulties in LFC. To persist in an ecosystem, symbiotic relations are predictable by an organism through imitators. Further, the dynamic behaviors of the controller optimized by ASOS, teaching learning-based optimization (TLBO), and differential evolution particle swarm optimization (DEPSO) are compared by extensive simulations in MATLAB/SIMULINK. Moreover, the supremacy of the proposed controller is performed through system dynamics comparison among PID, 2DOFPID, 3DOF-PID, and CC-3DOFPID-TID controllers. Finally, the sensitivity of the proposed controller has been proven through random load perturbation.