Local and metastatic tumor growth and membrane properties of LM fibroblasts in athymic (nude) mice

Glaucopsia in Emergency Department

“…Significant changes were also observed in the fatty acid composition of plasma membranes, microsomes, and mitochondria, with significant increases in saturated fatty acids of 16 and 18 carbons in length in the microsomes and mitochondria, accompanied by significant reductions were found in the 18 carbon-length unsaturated fatty acids. Ethanolamine-supplemented serum produced a significant reduction in the ratio of unsaturated:saturated fatty acids (Kier et al, 1988).…”

“…When the cells were injected into nude mice, the frequency of lung metastasis was 46% compared to frequencies of 74% and 68% for serum-and choline-fed cells, respectively. Choline-, DMAE-, and serum-cultured cells induced extensive, highly invasive metastases (Kier et al, 1988).…”

“…Carcinogenicity LM fibroblasts grown in the presence of monomethylethanolamine resulted in fewer lung metastases (42%) in the nude mouse relative to the metastases induced by serum-or choline supplemented serum (74% and 68%, respectively), possibly due to modifications of surface membrane components. Metastases from monomethylethanolamine-supplemented LM fibroblasts produced only embolus formation without invasion of local tissues (Kier and Schroeder, 1982;Kier et al, 1988).…”

“…In Kier et al (1988), tumors derived from LM cells grown on monomethylethanolamine supplemented serum and injected into nude mice had significant reductions in the specific activity of (Na + + K + )-ATPase (42.9 ± 3.6 nmol/minute per mg, p<0.05), NADH-dependent cytochrome-c reductase (234 ± 15 nmol/minute per mg, p<0.025), rotenone-insensitive NADH dependent cytochrome-c reductase (218 ± 17 nmol/minute per mg, p<0.025), and rotenone sensitive NADH-dependent cytochrome-c reductase (53 ± 5 nmol/minute per mg, p<0.05), relative to choline-supplemented serum. Significant changes were also observed in the fatty acid composition of plasma membranes, microsomes, and mitochondria, with significant increases in saturated fatty acids of 16 and 18 carbons in length in the microsomes and mitochondria, accompanied by significant reductions were found in the 18 carbon-length unsaturated fatty acids.…”