Local and Remote Postconditioning Decrease Intestinal Injury in a Rabbit Ischemia/Reperfusion Model

Mi, Yang; Dong, Jianxin; Li, Lubin; Che, H.; Ye, Jun; Song, Fang; Wang, Tao; Zhang, Jv-Wen

doi:10.1155/2016/2604032

Cited by 4 publications

(5 citation statements)

References 25 publications

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“…Plasma LDH and CK were within reference values during all time points and did not change over time. Contrary to the significant changes in oxidative stress levels found in studies investigating IPoC in a rat model [ 14 – 16 , 18 – 20 , 33 , 35 ], we found no differences between the treatment groups, contradicting an effect of IPoC on oxidative stress in the applied experimental model. A possible explanation may be that the low flow ischaemia implemented in this study elicited less oxidative stress than the complete occlusion of the cranial mesenteric artery (CMA) performed in the rat model.…”

Section: Discussioncontrasting

confidence: 99%

“…Intestinal IPoC studies in rat models have also demonstrated decreased levels of apoptotic markers in animals subjected to IPoC, reporting lower TUNEL positive cell counts [ 15 – 18 , 33 ], and cleaved caspase-3 expression [ 15 , 16 ]. A previous study in horses undergoing IPoC could demonstrate less epithelial denudation and lower paracellular permeability after experimental intestinal ischaemia [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

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Ischaemic postconditioning reduces apoptosis in experimental jejunal ischaemia in horses

Verhaar¹,

Buhr²,

Köckritz-Blickwede³

et al. 2021

BMC Vet Res

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Background Ischaemic postconditioning (IPoC) refers to brief periods of reocclusion of blood supply following an ischaemic event. This has been shown to ameliorate ischaemia reperfusion injury in different tissues, and it may represent a feasible therapeutic strategy for ischaemia reperfusion injury following strangulating small intestinal lesions in horses. The objective of this study was to assess the degree cell death, inflammation, oxidative stress, and heat shock response in an equine experimental jejunal ischaemia model with and without IPoC. Methods In this randomized, controlled, experimental in vivo study, 14 horses were evenly assigned to a control group and a group subjected to IPoC. Under general anaesthesia, segmental ischaemia with arterial and venous occlusion was induced in 1.5 m jejunum. Following ischaemia, the mesenteric vessels were repeatedly re-occluded in group IPoC only. Full thickness intestinal samples and blood samples were taken at the end of the pre-ischaemia period, after ischaemia, and after 120 min of reperfusion. Immunohistochemical staining or enzymatic assays were performed to determine the selected variables. Results The mucosal cleaved-caspase-3 and TUNEL cell counts were significantly increased after reperfusion in the control group only. The cleaved-caspase-3 cell count was significantly lower in group IPoC after reperfusion compared to the control group. After reperfusion, the tissue myeloperoxidase activity and the calprotectin positive cell counts in the mucosa were increased in both groups, and only group IPoC showed a significant increase in the serosa. Tissue malondialdehyde and superoxide dismutase as well as blood lactate levels showed significant progression during ischaemia or reperfusion. The nuclear immunoreactivity of Heat shock protein-70 increased significantly during reperfusion. None of these variables differed between the groups. The neuronal cell counts in the myenteric plexus ganglia were not affected by the ischaemia model. Conclusions A reduced apoptotic cell count was found in the group subjected to IPoC. None of the other tested variables were significantly affected by IPoC. Therefore, the clinical relevance and possible protective mechanism of IPoC in equine intestinal ischaemia remains unclear. Further research on the mechanism of action and its effect in clinical cases of strangulating colic is needed.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ischaemic postconditioning reduces apoptosis in experimental jejunal ischaemia in horses

Verhaar¹,

Buhr²,

Köckritz-Blickwede³

et al. 2021

BMC Vet Res

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“…The conclusion was that the protection of IPoC was related to reduced production of reactive oxygen species, enhanced activities of antioxidant systems, and inhibited apoptosis of intestinal mucosal cells. In a rabbit model, Yang et al 26 showed similar results, without any difference in the beneficial effects of LIPoC or RIPoC methods. This difference in relation to the rat model probably is due to a characteristic of the animal species.…”

Section: Abreu Ms Et Almentioning

confidence: 83%

“…Before abandoning the studies about alleviating the harmful effects of IRI, Our group of investigators decided to try the effects of IPoC methods. Until the present time, there has been a lot of evidence that RIPoC can reduce IRI in distant organs of rats, such as the intestines [17][18][19] , the myocardium 20,21 , the brain 22 , and the lungs 23 . Thus, RIPoC appears to be a promising method to explore for improving IRI damage, as it is a more straightforward procedure to be utilized in the complicated surgery of liver transplantation in humans.…”

Section: Discussionmentioning

confidence: 99%

Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia

et al. 2023

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Purpose: Acute mesenteric ischemia (AMI) is a condition in pediatric surgery that ranges from intestine necrosis to death. Ischemic postconditioning (IPoC) methods were developed to reduce the damage caused by revascularization. This study aimed to evaluate the efficacy of these methods in an experimental weaning rat model. Methods: Thirty-two 21-day-old Wistar rats were allocated into four groups according to the surgical procedure performed: control, ischemia-reperfusion injury (IRI), local (LIPoC) and remote IPoC (RIPoC). At euthanasia, fragments of the intestine, liver, lungs, and kidneys were submitted to histological, histomorphometric, and molecular analyses. Results:In the duodenum, intestines, and kidneys histological alterations promoted by IRI were reversed by remote postconditioning method. In the distal ileum, the histomorphometric alterations could be reversed by the postconditioning methods with more evident effects promoted by the remote method. The molecular analysis found that the levels of expression of Bax (proapoptotic) and Bcl-XL (antiapoptotic) genes in the intestine were increased by IRI. These alterations were equally reversed by the postconditioning methods, with more evident effects of the remote method. Conclusion: IPoC methods positively reduced the damage caused by IRI in weaning rats.

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“…Therefore, inhibition of oxidative stress and inflammatory response is widely accepted as the optimum therapeutic strategy for I/R injury ( 8 ). However accumulated clinical feedback reveals that serious side effects remain a vital challenge in dealing with oxidative damage, such as acute kidney injury ( 9 ), arrhythmia ( 10 , 11 ), hypertension ( 12 ), sepsis ( 13 ), and bowel infarct ( 14 ). Therefore, there is an increasing demand for potential natural products with lower toxicity and fewer side effects.…”

Section: Introductionmentioning

confidence: 99%

A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury

Zhang

Zhao

et al. 2022

Front. Nutr.

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We isolated and purified a novel polysaccharide from the root of Chuanminshen violaceum, namely, Chuanminshen violaceumis polysaccharide (CVP) and confirmed its structure and molecular weight. Furthermore, in vivo experiment, CVP’s protective effect against myocardial ischemia-reperfusion (I/R) injury in mice was evidenced by significantly reducing I/R-induced myocardial infarction (MI) size, decreasing the secretion of heart damage biomarkers, and improving cardiac function. Then, the myocardial anoxia/reoxygenation (A/R) injury model was established to mimic reperfusion injury. Noticeably, ferroptosis was the major death manner for A/R-damaged H9c2 cells. Meanwhile, CVP significantly inhibited ferroptosis by decreasing intracellular Fe2+ level, enhancing GPX4 expression, and suppressing lipid peroxidation to confront A/R injury. In conclusion, CVP, with a clear structure, ameliorated I/R injury by inhibiting ferroptosis.

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Local and Remote Postconditioning Decrease Intestinal Injury in a Rabbit Ischemia/Reperfusion Model

Cited by 4 publications

References 25 publications

Ischaemic postconditioning reduces apoptosis in experimental jejunal ischaemia in horses

Ischaemic postconditioning reduces apoptosis in experimental jejunal ischaemia in horses

Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia

A Novel Polysaccharide From Chuanminshen violaceum and Its Protective Effect Against Myocardial Injury

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