Local and systemic immune responses in pigs intramuscularly injected with an inactivated Mycoplasma hyopneumoniae vaccine

Marchioro, Silvana Beutinger; Maes, Dominiek; Flahou, Bram; Pasmans, Frank; Sacristán, Rubén Del Pozo; Vranckx, Katleen; Melkebeek, Vesna; Cox, Eric; Wuyts, Niels; Haesebrouck, Freddy

doi:10.1016/j.vaccine.2012.12.068

Cited by 45 publications

(32 citation statements)

References 25 publications

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“…infections in other domestic animals through immunization. However, it has not been possible to date to develop efficacious broadly protective vaccines to the closely related M. hyopneumoniae , the etiologic agent of porcine enzootic pneumonia [27], [35], [36]. M. hypopneumoniae shares the same basic pathophysiology and broad strain diversity as M. ovipneumoniae [2], [23], [27].…”

Section: Discussionmentioning

confidence: 99%

Safety and Immunogenicity of a Mycoplasma ovipneumoniae Bacterin for Domestic Sheep (Ovis aries)

et al. 2014

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BackgroundMortality from epizootic pneumonia is hindering re-establishment of bighorn sheep populations in western North America. Mycoplasma ovipneumoniae, a primary agent of this disease, is frequently carried asymptomatically by the domestic sheep and goats that constitute the reservoir of this agent for transmission to bighorn sheep. Our long-term objective is to reduce the risk of M. ovipneumoniae infection of bighorn sheep; one approach to this objective is to control the pathogen in its reservoir hosts.MethodsThe safety and immunogenicity of M. ovipneumoniae for domestic sheep was evaluated in three experimental immunization protocols: 1) live M. ovipneumoniae (50 ug protein); 2) killed M. ovipneumoniae (50 ug whole cell protein) in oil adjuvant; and 3) killed M. ovipneumoniae (250 ug whole cell protein) in oil adjuvant. Immunogenicity was assessed by two serum antibody measures: competitive enzyme-linked immunosorbent assay (cELISA) (experiments 1–3) and serum growth inhibition (Experiment 3). Passive immunogenicity was also assessed in the third experiment using the same assays applied to blood samples obtained from the lambs of immunized ewes.Results and ConclusionsAdverse reactions to immunization were generally minor, but local reactions were regularly observed at immunization sites with bacterins in oil adjuvants. No evidence of M. ovipneumoniae specific antibody responses were observed in the first or second experiments and no resistance to colonization was observed in the first experiment. However, the ewes in the third experiment developed strong cELISA serum antibody responses and significant serum M. ovipneumoniae inhibition activity, and these responses were passively transferred to their lambs. The results of these trials indicate that immunization with relatively large antigenic mass combined with an adjuvant is capable of inducing strong active antibody responses in ewes and passively immunizing lambs.

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Section: Discussionmentioning

confidence: 99%

Safety and Immunogenicity of a Mycoplasma ovipneumoniae Bacterin for Domestic Sheep (Ovis aries)

et al. 2014

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“…Although the vaccine employed was different, cells producing IFN‐γ in peripheral blood after MPS vaccination are rare (Marchioro et al . ), which may obscure the expression level of IFN‐γ mRNA. Another possibility is that host cells other than circulating blood cells are responsible for the production of IFN‐γ.…”

Section: Discussionmentioning

confidence: 99%

Genetic selection for resistance to mycoplasmal pneumonia of swine (MPS) in the Landrace line influences the expression of soluble factors in blood after MPS vaccine sensitization

Shimazu

Borjigin

Katayama

et al. 2013

Animal Science Journal

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We recently developed a Landrace line that is resistant to mycoplasmal pneumonia of swine (MPS) infection by genetic selection for five generations, and we reported that the immunophenotype of this line is different from that of the non-selected line in terms of changes in peripheral blood leukocyte population after MPS vaccination. This study followed up previous findings demonstrating changes in soluble factors in blood, namely, hormones, Mycoplasma hyopneumoniae-specific immunoglobulin G (IgG), and cytokines. These two lines were injected with MPS vaccine on days -7 and 0 after blood sampling on those days, and blood samples were collected on days -14, -7, 0, 2, 7 and 14. We found changes in the levels of many hormones and cytokines in both lines. However, we found that only growth hormone (GH) and interferon (IFN)-γ levels were statistically different between these two lines. GH concentration was reduced (day 0) and IFN-γ concentration was increased (day 14) in the MPS-selected line compared with the non-selected line, despite unchanged IFN-γ messenger RNA expression in blood cells. Although detailed mechanisms underlying these phenotypes remain unsolved, these traits would be useful to improve MPS resistance in pig production and provide an insight into MPS infection.

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“…The exact mechanisms of protection are not yet fully understood. Studies suggest that systemic cell‐mediated immune responses are important for protection (Marchioro et al., ). Vranckx et al.…”

Section: Prevention and Controlmentioning

confidence: 99%

“…Therefore, it can not only evade the immune system but could disseminate to internal organs and persist within its host without causing disease. Isolation of M. hyopneumoniae from other tissue than lung has in fact been reported (Le Carrou, Laurentie, Kobisch, & Gautier-Bouchardon, 2006;Marchioro et al, 2013;Marois, Le Carrou, Kobisch, & Gautier-Bouchardon, 2007).…”

Section: Comparative Studies With Other Mycoplasmasmentioning

confidence: 99%

Update onMycoplasma hyopneumoniaeinfections in pigs: Knowledge gaps for improved disease control

Maes

Sibila

Kuhnert

et al. 2017

Transbound Emerg Dis

214

229

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SummaryMycoplasma hyopneumoniae (M. hyopneumoniae) is the primary pathogen of enzootic pneumonia, a chronic respiratory disease in pigs. Infections occur worldwide and cause major economic losses to the pig industry. The present paper reviews the current knowledge on M. hyopneumoniae infections, with emphasis on identification and analysis of knowledge gaps for optimizing control of the disease. Close contact between infected and susceptible pigs is the main route of M. hyopneumoniae transmission. Management and housing conditions predisposing for infection or disease are known, but further research is needed to better understand M. hyopneumoniae transmission patterns in modern pig production systems, and to assess the impor-

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Local and systemic immune responses in pigs intramuscularly injected with an inactivated Mycoplasma hyopneumoniae vaccine

Cited by 45 publications

References 25 publications

Safety and Immunogenicity of a Mycoplasma ovipneumoniae Bacterin for Domestic Sheep (Ovis aries)

Safety and Immunogenicity of a Mycoplasma ovipneumoniae Bacterin for Domestic Sheep (Ovis aries)

Genetic selection for resistance to mycoplasmal pneumonia of swine (MPS) in the Landrace line influences the expression of soluble factors in blood after MPS vaccine sensitization

Update onMycoplasma hyopneumoniaeinfections in pigs: Knowledge gaps for improved disease control

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