Local application of adipose-derived mesenchymal stem cells supports the healing of fistula: prospective randomised study on rat model of fistulising Crohn’s disease

Ryska, O; Šerclová, Zuzana; Měšťák, Ondřej; Matoušková, Eva; Veselý, Pavel; Mrázová, Iveta

doi:10.1080/00365521.2017.1281434

Cited by 16 publications

(21 citation statements)

References 41 publications

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“…Finally, the full histological remission was not observed in any dog [107]. Moreover, the local application of ASCs was associated with a significantly higher rate of fistula closure in a rat model of perianal fistulas [111].…”

Section: Animal Modelsmentioning

confidence: 89%

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In Vitro Cultures of Adipose-Derived Stem Cells: An Overview of Methods, Molecular Analyses, and Clinical Applications

Jankowski

Dompe

Sibiak

et al. 2020

Cells

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Adipose-derived stem cells (ASCs) exhibiting mesenchymal stem cell (MSC) characteristics, have been extensively studied in recent years. Because they have been shown to differentiate into lineages such as osteogenic, chondrogenic, neurogenic or myogenic, the focus of most of the current research concerns either their potential to replace bone marrow as a readily available and abundant source of MSCs, or to employ them in regenerative and reconstructive medicine. There is close to consensus regarding the methodology used for ASC isolation and culture, whereas a number of molecular analyses implicates them in potential therapies of a number of pathologies. When it comes to clinical application, there is a range of examples of animal trials and clinical studies employing ASCs, further emphasizing the advancement of studies leading to their more widespread use. Nevertheless, in vitro studies will most likely continue to play a significant role in ASC studies, both providing the molecular knowledge of their ex vivo properties and possibly serving as an important step in purification and application of those cells in a clinical setting. Therefore, it is important to consider current methods of ASC isolation, culture, and processing. Furthermore, molecular analyses and cell surface properties of ASCs are essential for animal studies, clinical studies, and therapeutic applications of the MSC properties.

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Section: Animal Modelsmentioning

confidence: 89%

“…Local application of adipose-derived mesenchymal stem cells supports the healing of fistula: prospective randomised study on rat model of fistulising Crohn's disease [111] Prospective, randomized, controlled study 32 rats Perianal fistula model Allogeneic Ryska,…”

Section: Human Ascsmentioning

confidence: 99%

In Vitro Cultures of Adipose-Derived Stem Cells: An Overview of Methods, Molecular Analyses, and Clinical Applications

Jankowski

Dompe

Sibiak

et al. 2020

Cells

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“…In previous human studies for other conditions, systemic adverse effects such as infections and viral reactivation secondary to immune suppression occurred less frequently with local application of cells as compared to systemic application [73, 74]. Although both IP and intravenous (IV) routes of administration had equivalent efficacy in an animal model, many cells become entrapped in the lungs after IV injection, leading to a reduction in the number of cells available for therapeutic purposes [14, 75, 76].…”

Section: Clinical Applicationsmentioning

confidence: 99%

Stem cell therapy in necrotizing enterocolitis: Current state and future directions

Drucker

McCulloh

et al. 2018

Seminars in Pediatric Surgery

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Stem cell therapy is a promising treatment modality for necrotizing enterocolitis. Among the many promising stem cells identified to date, it is likely that mesenchymal stem cells will be the most useful and practical cell-based therapies for this condition. Using acellular components such as exosomes or other paracrine mediators are promising as well. Multiple mechanisms are likely at play in the positive effects provided by these cells, and further research is underway to further elucidate these effects.

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“…AD-MSCs were used in this induced CD mice model where systemic infusion of AD-MSCs improved the clinical and histopathologic severity of colitis, reduced diarrhea and inflammation, and increased mice survival. This study found that AD-MSCs down-regulated Th1-driven inflammatory responses which subsequently decreased the production of inflammatory cytokines and chemokines [19]. In another study dogs with perianal fistulas were treated intralesionally with 2 × 107 human embryonic stem cell derived MSCT.…”

Section: Animal Studiesmentioning

confidence: 81%

Crohn’s Disease: Hematopoietic and Mesenchymal Stem Cell Therapy as Potential Treatment

2020

JCRC

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Purpose: The purpose of this work was to comprehensively review literature to determine the safety and efficacy of stem cells for the treatment of Crohn’s disease and compare the effects of hematopoietic stem cells (HSCs) to that of mesenchymal stem cells (MSCs). Results: The data search included 5 animal models and clinical trials found on PubMed. The 5 studies included for HSCs and the 15 trails for MSCs found them to be a safe and effective as a treatment option (allogeneic and autologous bone marrow and adipose derived) for CD except for one multicenter randomized trial for HSC therapy. Most trials investigate the impact of stem cells specifically on perianal fistula, a common occurrence in patients with CD. Conclusion: While both HSCs and MSCs proved their safety and efficacy, MSC studies showed a greater therapeutic effect over HSCs. Adipose MSCs and Bone Marrow Derived MSCs reached similar clinical healing rates, with studies backing support for both sides. Across the board, autologous stem cell transplants proved to be safer and more effective compared to their allogeneic counterparts.

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Local application of adipose-derived mesenchymal stem cells supports the healing of fistula: prospective randomised study on rat model of fistulising Crohn’s disease

Cited by 16 publications

References 41 publications

In Vitro Cultures of Adipose-Derived Stem Cells: An Overview of Methods, Molecular Analyses, and Clinical Applications

In Vitro Cultures of Adipose-Derived Stem Cells: An Overview of Methods, Molecular Analyses, and Clinical Applications

Stem cell therapy in necrotizing enterocolitis: Current state and future directions

Crohn’s Disease: Hematopoietic and Mesenchymal Stem Cell Therapy as Potential Treatment

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