Local Expression of Interleukin-17a is Correlated with Nasal Eosinophilia and Clinical Severity in Allergic Rhinitis

Abstract

Interleukin (IL)-17A is a major cytokine produced by Th17 cells, which are associated with chronic inflammations. The local expression of IL-17A in allergic rhinitis (AR) remains to be characterized. We sought to determine the role of IL-17A expression in human inferior turbinate mucosa in the pathophysiology of AR. Inferior turbinate mucosa was sampled from medical treatment–resistant, surgery-required patients with perennial AR (PAR, n = 21), nonallergic rhinitis with eosinophilia syndrome (NARES, n = 7), and nonallergic hypertrophic rhinitis (HR, n = 13). IL-17A expression was determined with immunohistochemical staining. The mean number of IL-17A+ cells and eosinophils per field were counted. Total serum immunoglobulin E (IgE) levels, blood eosinophil count, and forced expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) ratio were also examined in each patient. IL-17A was primarily expressed in infiltrating inflammatory cells. The number of IL-17A+ cells in nasal mucosa was significantly higher in the PAR group compared with HR (p = 0.002) and NARES (p = 0.021) groups. There was a significant and positive correlation between the number of IL-17A+ cells and total nasal symptom score (rho = 0.403; p = 0.011), especially sneezing score (rho = 0.471; p = 0.003). The number of IL-17A+ cells was significantly and positively correlated with the degree of eosinophil infiltration (rho = 0.623; p < 0.001), but not with total serum IgE levels (rho = 0.284; p = 0.098), blood eosinophil counts (rho = 0.302; p = 0.056), or FEV1/FVC ratio (rho = 0.092; p = 0.569). The present study provides evidence that IL-17A expression in the nasal mucosa is associated with the pathophysiology of AR, including disease severity and nasal eosinophilia.

Interleukin‐9 and interleukin‐17C in chronic rhinosinusitis

“…The number of positively-stained cells were counted in 5 consecutive fields under ×400 magnification per section. 7 Each specimen was quantified in duplicate by counting 2 sections per specimen. The average of the 2 counts was then reported.…”

Cytokine Modulation by IL-35 in Mice with Allergic Rhinitis

“…E pstein-Barr virus-induced gene 3 and the p35 subunit of interleukin (IL) 12 have been reported to form a heterodimeric hematopoietin, IL-35, which, in 2007, was identified as an additional antiinflammatory and immunosuppressive cytokine. 1 IL-35 generates regulatory T (Treg) cells that express IL-35 but do not express Foxp3, transforming growth factor (TGF)-␤, or IL-10 (IL-35 producing inducible regulatory T cells [iTreg35 cells]).…”

Biomarcadores Na Rinite Alérgica: Qual a Importância E Potencial Diagnóstico?

“…A IL-9, na mucosa nasal, estimula a infiltração tecidual pelos mastócitos e eosinófilos, sendo sobrerregulada na mucosa nasal durante a temporada da polinização (NOURI-ARIA et al, 2005). Além disso, seus níveis séricos têm relação significativa com a severidade dos sintomas, demonstrada por uma relação moderada positiva entre IL-9 e prurido nasal (r=0,563, p<0,001), espirros (r=0,543, p<0,001), rinorreia (r=0,706, p<0,001) e uma pobre correlação negativa com a severidade da obstrução nasal (r=-0.0371, p=0,02).…”

“…Houve correlação positiva entre IL-17A e sintomas nasais (r=0.43, p=0.011) e com contagens de eosinófilos (r=0.623, p<0.001). Estes dados associam a IL-17A com a patofisiologia da RA, incluindo severidade da doença e eosinofilia nasal, podendo servir como futuro biomarcador na RA (MAKIHARA et al, 2014).…”