Local fibrinolysis and aspiration of intracerebral hematomas in rats. An experimental study using MR monitoring

Deinsberger, Wolfgang; Hartmann, Marius; Vogel, Johannes; Jansen, Olav; Kuschinsky, Wolfgang; Sartor, Klaus; Dk, Böker

doi:10.1080/01616412.1998.11740530

Cited by 12 publications

(8 citation statements)

References 21 publications

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“…Intrastriatal autologous whole blood injection in mice was previously described [23,24]. Briefly, after tracheal intubation, the animal's head was secured in a stereotactic frame, and a burr hole was created 2.2 mm left lateral to the bregma.…”

Section: Methodsmentioning

confidence: 99%

Progesterone Improves Neurobehavioral Outcome in Models of Intracerebral Hemorrhage

Lei

Wang²,

Jeong³

et al. 2015

Neuroendocrinology

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In models of acute brain injury, progesterone improves recovery through several mechanisms including modulation of neuroinflammation. Secondary injury from neuroinflammation is a potential therapeutic target after intracerebral hemorrhage (ICH). For potential translation of progesterone as a clinical acute ICH therapeutic, the present study sought to define efficacy of exogenous progesterone administration in ICH-relevant experimental paradigms. Young and aged C57BL/6 male, female, and ovariectomized (OVX) mice underwent left intrastriatal collagenase (0.05-0.075 U) or autologous whole blood (35 μl) injection. Progesterone at varying doses (4-16 mg/kg) was administered at 2, 5, 24, 48, and 72 h after injury. Rotarod and Morris water maze latencies were measured on days 1-7 and days 28-31 after injury, respectively. Hematoma volume, brain water content (cerebral edema), complementary immunohistochemistry, multiplex cytokine arrays, and inflammatory proteins were assessed at prespecified time points after injury. Progesterone (4 mg/kg) administration improved rotarod and water maze latencies (p < 0.01), and decreased cerebral edema (p < 0.05), microglial proliferation, and neuronal loss (p < 0.01) in young and aged male, young OVX, and aged female mice. Brain concentration of proinflammatory cytokines and Toll-like receptor-associated proteins were also decreased after progesterone (4 mg/kg) treatment (p < 0.01). Progesterone-treated young female mice showed no detectable effects. Exogenous progesterone improved short- and long-term neurobehavioral recovery and modulated neuroinflammation in male and OVX mice after ICH. Future studies should validate these findings, and address timing and length of administration before translation to clinical trial.

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Section: Methodsmentioning

confidence: 99%

Progesterone Improves Neurobehavioral Outcome in Models of Intracerebral Hemorrhage

Lei

Wang²,

Jeong³

et al. 2015

Neuroendocrinology

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“…This is most likely the reason why we were not able to demonstrate a relevant DWI-volumetric reduction of the clot after lysis, although previous groups reported a reliable significant modification in hematoma size [1,5]. According to the literature hematoma reduction of approximately 30% is achieved with this technique [4,5]. Due to the fact that blood was aspired we assume but cannot prove that hematoma volume was reduced by rt-PA facilitated evacuation.…”

Section: Discussionmentioning

confidence: 56%

“…Experimental and clinical studies which highlight the question whether hematoma evacuation can positively influence metabolism and perfusion in order to limit secondary neuronal damage in healthy tissue are rare [1][2][3][4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

MRI of the Perihemorrhagic Zone in a Rat ICH Model: Effect of Hematoma Evacuation

et al. 2008

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“…The obvious disadvantage to this technique is that it does not emulate small vessel rupture, which is thought to be the [8]. Blood ''reflux'' has been largely resolved by the development of a 'double injection' technique by Deissberger et al [9], whereby a small amount of blood is infused and allowed to clot along the needle track with subsequent infusion of the remainder of the blood volume. Blood injection allows for clinically relevant comparisons in that the mechanistic effects examined are strictly related to the presence of blood within parenchymal brain tissue; however, there is no opportunity to evaluate rebleeding or the effects of microvascular breakdown.…”

Section: Autologous Blood Injection Modelmentioning

confidence: 99%

“…Finally, the MRI was validated in the acute (under 6 h) [44] and hyperacute (30 min) phases of ICH with histological hemorrhage size in an autologous blood injection rabbit model [45]. Several projects have employed imaging as an outcome measure for therapeutic interventions including the calcium channel antagonist levemopamil [131], a variety of antioxidants [61,114], the anti-inflammatory fuicodan [132], a neurotrophic factor axokine [133], fibrinolysis for clot evacuation [9], and long-term outcome measures [34]. Imaging provides a useful mechanism to study progression of secondary injury in a living animal, and reduces the total number of animals necessary to produce meaningful results (Fig.…”

Section: Imaging Techniquesmentioning

confidence: 99%

Preclinical Models of Intracerebral Hemorrhage: A Translational Perspective

2007

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Intracerebral hemorrhage (ICH) is a devastating and relatively common disease affecting as many as 50,000 people annually in the United States alone. ICH remains associated with poor outcome, and approximately 40-50% of afflicted patients will die within 30 days. In reports from the NIH and AHA, the importance of developing clinically relevant models of ICH that will extend our understanding of the pathophysiology of the disease and target new therapeutic approaches was emphasized. Traditionally, preclinical ICH research has most commonly utilized two paradigms: clostridial collagenase-induced hemorrhage and autologous blood injection. In this article, the use of various species is examined in the context of the different model types for ICH, and a mechanistic approach is considered in evaluating the numerous breakthroughs in our current fund of knowledge. Each of the model types has its inherent strengths and weaknesses and has the potential to further our understanding of the pathophysiology and treatment of ICH. In particular, transgenic rodent models may be helpful in addressing genetic influences on recovery from ICH.

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Local fibrinolysis and aspiration of intracerebral hematomas in rats. An experimental study using MR monitoring

Cited by 12 publications

References 21 publications

Progesterone Improves Neurobehavioral Outcome in Models of Intracerebral Hemorrhage

Progesterone Improves Neurobehavioral Outcome in Models of Intracerebral Hemorrhage

MRI of the Perihemorrhagic Zone in a Rat ICH Model: Effect of Hematoma Evacuation

Preclinical Models of Intracerebral Hemorrhage: A Translational Perspective

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