Local imipenem activity against Pseudomonas aeruginosa decreases in vivo in the presence of siliconized latex

Pichardo, C.; Conejo, M. Carmen; Docobo-Pérez, Fernando; Velasco, C.; López-Rojas, Rafael; Garcı́a, Isabel; Pachón-Ibáñez, María Eugenia; Rodríguez, Juan Miguel Díaz; Pachón, Jerónimo; Pascual, Álvaro

doi:10.1007/s10096-010-1084-y

Cited by 3 publications

(4 citation statements)

References 7 publications

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“…The present study evaluates the in vivo therapeutic efficacies of daptomycin and vancomycin against two biofilm-producing MRSE strains by using a murine model of foreign-body infection described previously (18). Using this model, both antimicrobials reduced the bacterial concentrations in catheter and liver and increased the numbers of sterile blood cultures and survival rates with any strain.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Daptomycin versus Vancomycin in an Experimental Model of Foreign-Body and Systemic Infection Caused by Biofilm Producers and Methicillin-Resistant Staphylococcus epidermidis

Domínguez-Herrera

Docobo-Pérez

López-Rojas

et al. 2012

Antimicrob Agents Chemother

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Staphylococcus epidermidis is a frequent cause of device-associated infections. In this study, we compared the efficacy of daptomycin versus vancomycin against biofilm-producing methicillin-resistant S. epidermidis (MRSE) strains in a murine model of foreign-body and systemic infection. Two bacteremic biofilm-producing MRSE strains were used (SE284 and SE385). The MIC of daptomycin was 1 mg/liter for both strains, and the MICs of vancomycin were 4 and 2 mg/liter for SE284 and for SE385, respectively. The in vitro bactericidal activities of daptomycin and vancomycin were evaluated by using time-kill curves. The model of foreign-body and systemic infection of neutropenic female C57BL/6 mice was used to ascertain in vivo efficacy. Animals were randomly allocated into three groups (n ‫؍‬ 15): without treatment (controls) or treated with daptomycin at 50 mg/kg/day or vancomycin at 440 mg/kg/day. In vitro, daptomycin showed concentration-dependent bactericidal activity, while vancomycin presented time-dependent activity. In the experimental in vivo model, daptomycin and vancomycin decreased liver and catheter bacterial concentrations (P < 0.05) and increased the survival and the number of sterile blood cultures (P < 0.05) using both strains. Daptomycin produced a reduction in the bacterial liver concentration higher than 2.5 log 10 CFU/g compared to vancomycin using both strains, with this difference being significant (P < 0.05) for infection with SE385. For the catheter bacterial concentrations, daptomycin reduced the concentration of SE284 3.0 log 10 CFU/ml more than did vancomycin (P < 0.05). Daptomycin is more effective than vancomycin for the treatment of experimental foreign-body and systemic infections by biofilmproducing methicillin-resistant S. epidermidis. Staphylococcus epidermidis is a common nosocomial and health care-associated pathogen in several infections, causing important morbidity, mortality, and/or health care costs. Thus, it is the most important cause of infections of orthopedic prostheses, accounting for approximately 40% of all cases, and between 30% and 50% of catheter-related bacteremias are caused by coagulasenegative Staphylococcus (CNS) strains (19,26). Other severe and/or frequent nosocomial and health care-associated infections are also caused by CNS, being the etiology in the 22.7% of endocarditis infections (9, 13) and in 37 to 78% of cerebrospinal fluid shunt-associated infections (4, 11).Moreover, the high frequency of methicillin-resistant S. epidermidis (MRSE) is an important therapeutic problem.

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Section: Discussionmentioning

confidence: 99%

Efficacy of Daptomycin versus Vancomycin in an Experimental Model of Foreign-Body and Systemic Infection Caused by Biofilm Producers and Methicillin-Resistant Staphylococcus epidermidis

Domínguez-Herrera

Docobo-Pérez

López-Rojas

et al. 2012

Antimicrob Agents Chemother

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“…A modification of the model has been used by Pichardo et al. to evaluate treatments for infections of urinary catheters [192]. Siliconized latex (SL) or silicone (S) catheter sections were implanted in the peritoneal cavity of C57BL/6 mice in one lateral abdominal wall.…”

Section: Other Foreign Body Infectionsmentioning

confidence: 99%

Evaluation of Pseudomonas aeruginosa pathogenesis and therapeutics in military‐relevant animal infection models

Bobrov

Getnet

Swierczewski

et al. 2021

APMIS

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Modern combat-related injuries are often associated with acute polytrauma. As a consequence of severe combat-related injuries, a dysregulated immune response results in serious infectious complications. The gram-negative bacterium Pseudomonas aeruginosa is an opportunistic pathogen that often causes life-threatening bloodstream, lung, bone, urinary tract, and wound infections following combat-related injuries. The rise in the number of multidrug-resistant P. aeruginosa strains has elevated its importance to civilian clinicians and military medicine. Development of novel therapeutics and treatment options for P. aeruginosa infections is urgently needed. During the process of drug discovery and therapeutic testing, in vivo testing in animal models is a critical step in the bench-to-bedside approach, and required for Food and Drug Administration approval. Here, we review current and past literature with a focus on combat injuryrelevant animal models often used to understand infection development, the interplay between P. aeruginosa and the host, and evaluation of novel treatments. Specifically, this review focuses on the following animal infection models: wound, burn, bone, lung, urinary tract, foreign body, and sepsis.

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“…This study evaluated the in vivo therapeutic efficacy of ceftaroline and vancomycin against two biofilm-producing MRSE strains using a previously described foreign-body and systemic infection murine model [20,29]. Both antimicrobials reduced bacterial concentrations in the catheter and liver and increased survival with both strains; regarding the foreign-body infection, ceftaroline reduced the bacterial concentration of both strains by ca.…”

Section: Discussionmentioning

confidence: 99%

“…A modified murine foreign-body infection model [20,29] was used to evaluate the efficacy of ceftaroline and vancomycin. Briefly, mice were rendered neutropenic and then 1 cm of sterile polyfluorinated ethylene-propylene catheter was aseptically implanted into the abdominal cavity.…”

Section: Animal Modelmentioning

confidence: 99%

Efficacy of ceftaroline versus vancomycin in an experimental foreign-body and systemic infection model caused by biofilm-producing methicillin-resistant Staphylococcus epidermidis

Domínguez-Herrera

López-Rojas

Smani

et al. 2016

International Journal of Antimicrobial Agents

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Local imipenem activity against Pseudomonas aeruginosa decreases in vivo in the presence of siliconized latex

Cited by 3 publications

References 7 publications

Efficacy of Daptomycin versus Vancomycin in an Experimental Model of Foreign-Body and Systemic Infection Caused by Biofilm Producers and Methicillin-Resistant Staphylococcus epidermidis

Efficacy of Daptomycin versus Vancomycin in an Experimental Model of Foreign-Body and Systemic Infection Caused by Biofilm Producers and Methicillin-Resistant Staphylococcus epidermidis

Evaluation of Pseudomonas aeruginosa pathogenesis and therapeutics in military‐relevant animal infection models

Efficacy of ceftaroline versus vancomycin in an experimental foreign-body and systemic infection model caused by biofilm-producing methicillin-resistant Staphylococcus epidermidis

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