LOCAL IMMUNE RESPONSE IN MICE TO VIBRIO CHOLERAE

Abstract

Summary Cholera immunization schedules were investigated in mice, with emphasis placed on obtaining an immune response in the intestine. The most effective schedule for producing a good local response was found to be several orally‐given priming doses of the organism followed after 14 days by an intravenous boosting dose. Major differences between the immune responses in the spleen and the intestine were noted.

Efficacy of Intraduodenal, Oral and Parenteral Boosting in Inducing Intestinal Mucosal Immunity to Cholera Toxin in Rats

“…However, the marked anti-CTx-IgA antibody titer in bile following oral boosting was not anticipated. Bloom and Rowley showed that oral immunization followed by a parented boost yields a vigorous intestinal antibody response to CTx (15). Whether or not these antibody titers are in response to the primary ID vaccination in the present study is unresolved since we did not measure biliary titers prior to Day 19. i.e.. before the oral boost.…”

The Effect of Antibody on the Intestinal Absorption of Macromolecules and on Intestinal Permeability in Adult Mice

“…Mice immunised orally to induce high levels of intestinal IgA antibody [11], surprisingly showed an increased permeability compared to nor mal mice when challenged orally with the antiserum-antigen mixtures (p = 0.013), and also when given the antigen mixed with normal mouse se rum or NIJ (p = 0.024; table IV). This paradox can perhaps be explained by the presence of other anti body classes (IgM/IgG) that are undoubtedly present [12], and the high antigenic dose required to produce the phenomenon could have overridden any func tional importance of the IgA antibodies. Indeed, some modulation of the effect by IgA was observed when the BA fed to immune mice was mixed with IIJ (previously incubated at 37 °C for 1 h to degrade any IgM or IgG present [13] compared with that given with NIJ (p = 0.040; table IV).…”

Enteric Immunization: Promises and Challenges

“…This kind of synergy was observed in comparative studies of secretory IgA antibody responses in Swedish and Pakis tani women vaccinated subcutaneously with cholera vaccine [40], Whereas the Pakistani women, who had had previous natural expo sure to cholera, had a secretory IgA response in milk and saliva to the subcutaneous injec tion, Swedish women did not. Similarly, the best local immune responses in mice vacci nated with V cholerae were obtained after several oral priming doses followed by an intravenous dose of the organisms [41]. In human subjects, two booster doses of subcuta neously administered killed typhoid vaccine significantly increased the specific intestinal IgA antibody response in those persons primed with the live oral vaccine, S. typhi Ty21a.…”