Local immune response to experimental<i>Fasciola hepatica</i>infection in sheep

Chauvin, Alain; Boulard, Chantal

doi:10.1051/parasite/1996033209

Cited by 37 publications

(25 citation statements)

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“…The same IgM responses were noted in other hosts such as sheep (Chauvin et al, 1996) and to other trematodes such as Schistosoma mansoni (Khalif et al, 1985). Thus IgM may act as a blocking isotype during fasciolasis.…”

Section: Discussionsupporting

confidence: 60%

“…The local immune response in the area surrounding young flukes during migration through the liver which may play a role in resistance mechanisms has been explored less. Chauvin et al, (1996) showed that the migratory tunnels produced by juvenile flukes in sheep liver appeared as focal areas of necrosis surrounded by infiltrating inflammatory cells, in particular numerous macrophages, eosinophils and OvCD4 + lymphocytes. Moreover, during chronic infection and re-infection in sheep, the number of CD8 + lymphocytes became greater than that of CD4 + (Meeusen et al, 1995).…”

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confidence: 99%

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Local hepatic immune response in rats during primary infection withFasciola hepatica

et al. 2000

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Summary:The distribution of lymphocyte subpopulations (TCD4 + , TCD8 + , TCD43 + and lg + cells), macrophages and eosinophils were analysed in the inflammatory infiltrates associated with hepatic lesions and in hepatic lymph nodes (HLN) from rats experimentally infected with F. hepatica and necropsied 1, 2, 3, 4, 6 and 8 week post infection (WPI). We also investigated the fixation of immunoglobulin isotypes on migrating flukes in the liver. As early as 1WPI, portal tract areas surrounding migratory tunnels were

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Section: Discussionsupporting

confidence: 60%

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confidence: 99%

Local hepatic immune response in rats during primary infection withFasciola hepatica

et al. 2000

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“…Resistance to reinfection and host response to F. hepatica vary greatly between species [12]. Several studies have investigated immune response to F. hepatica in sheep [4,5,16]. These studies reported that sheep do not develop resistance to reinfection.…”

Section: Introductionmentioning

confidence: 99%

“…Local and systemic immune responses have been studied in sheep infected and reinfected with F. hepatica [4,5,16]. More recently, the effects of infection and treatment on subsequent challenge have been studied in cattle [13].…”

Section: Introductionmentioning

confidence: 99%

Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

Pérez¹,

Ortega²,

Bravo³

et al. 2005

Vet. Res.

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-The phenotype of the hepatic inflammatory infiltrate and hepatic lymph nodes (HLN) was analysed in lambs primarily and challenge infected with Fasciola hepatica. Group 1 was primarily and challenge infected with two doses of 200 metacercariae (mc) each and was nontreated. Trickle infection was administered to five groups: group 2 was challenge infected and nontreated; group 3 was primarily infected and non-treated; group 4 was primarily infected and treated with triclabendazole (TCBZ) at 12 weeks postinfection (wpi); group 5 was treated at 4 wpi and challenge infected and group 6 was treated at 12 wpi and challenge infected. An uninfected group was used as the control. The distribution of T cell subpopulations (CD3, CD4 and CD8), and B cells (CD79α, IgM, IgG) was analysed. The hepatic inflammatory infiltrate was represented mainly by CD3 and CD4 T cells, and B cells (CD79α, IgG). These infiltrates were more severe (P < 0.05) in primarily (group 3) or challenge (groups 2, 5 and 6) trickle infected lambs than in the group single challenge infected (group 1). Cellular changes in HLN consisted in an increase of CD4 over CD8 T cells and an increase of B cells and IgG + plasma cells, and they were more severe in primarily and challenge trickle infected groups than in the group infected with two larger doses of mc, although significant differences were not found with respect to all challenge trickle infected groups. The strong local cellular and humoral immune responses did not protect against subsequent infections, neither in non-treated lambs (group 2) nor in lambs treated with TCBZ at 4 wpi (group 5) or 12 wpi (group 6).

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“…The release of cytokines by lymphocytes is triggered by antigen recognition, which in turn initiates and regulates cell-to-cell communication. Granuloma formation, Ig isotype and the massive eosinophilia observed after 14 days of infection with F. hepatica indicate an oriented Th2 immune response [4,17,26]. However, before day 14 postinfection (pi), such data are unavailable and it is difficult to analyse the immune response by these means.…”

Section: Introductionmentioning

confidence: 99%

Early hepatic immune response in rats infected with Fasciola hepatica

et al. 2002

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-We investigated the phenotype of the T cells (CD4 + and CD8 + ) that produced Th1 (IFN-γ ) and Th2 cytokines (IL-4 and IL-10) during the first two weeks of experimental fasciolosis in rats. We also followed the kinetics of the cytokine and proliferative responses of hepatic mononuclear cells (HMNC) over the same period. We found that HMNC were more numerous in the infected animals than in the controls. The percentage of CD4 + cells increased significantly after infection, whereas the percentage of CD8 + cells did not change. Moreover, the frequency of the cells producing (CP) cytokine changed after infection. The frequency of CP IFN-γ on 7 days postinfection (pi) was similar to that in control animals. However, the frequency of CP IFN-γ was clearly lower on day 14 pi, whereas the frequency of CP IL-4 and CP IL-10 had increased. The CP IL-10-were mostly CD4 + . Mitogenic stimulation (phorbol myristate acetate/ionomycin) of HMNC led to an increase in the amounts of the Th2 cytokines in the supernatant on days 7 and 14 pi, with the increase more pronounced on day 14. In contrast, IFN-γ levels also increased by day 7 pi but then decreased to below control levels by day 14. In addition, HMNC proliferation in response to mitogen followed a similar pattern to IFN-γ production. These findings suggested that, during the first 2 weeks of infection, F. hepatica induced a transient Th0 cytokine profile followed by downregulation of the cellular response and the induction of a Th2 cytokine profile.Fasciola hepatica / rat / cytokine / liver / lymphocytes Résumé -Réponse immunitaire hépatique précoce chez le rat infesté par Fasciola hepatica. Nous avons caractérisé le phénotype des sous-populations lymphocytaires T (CD4 + , CD8 + ) qui produisent des cytokines de type Th1 (IFN-γ ) et Th2 (IL-4, IL-10) au cours de l'infestation expérimen-tale par F. hepatica chez le rat (phase précoce). Nous avons aussi suivi la production des cytokines et (33) 2 47 42 77 74; e-mail: sibille@tours.inra.fr la réponse proliférative des cellules mononucléaires hépatiques (CMNH) après une stimulation in vitro par des mitogènes (phorbol myristate acetate) et par des antigènes spécifiques (les produits d'excrétion-sécrétion de F. hepatica). Le pourcentage des cellules CD4 + augmente après infestation tandis que celui des cellules CD8 + demeure inchangé. Après infestation, la fréquence des cellules productrices (CP) de cytokine change. Tandis qu'au 7 e jour après infestation (JAI) la fréquence des CP d'IFN-γ reste identique à celles des animaux témoins, elle est nettement plus basse au 14 e JAI ; la fréquence des CP d'IL-4 et des CP d'IL-10 augmente (surtout les CD4 + IL-10 + , notamment au 14 e JAI). En outre, dans les surnageants de culture des CMNH, la production de cytokines de type Th2 est accrue au 7 e JAI et plus importante encore au 14 e JAI. Le niveau de production d'IFN-γ augmente au 7 e JAI mais décroît jusqu'à devenir inférieure à celle du témoin au 14 e JAI. De plus, la ré-ponse proliférative est plus importante au 7 e JAI mais...

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Local immune response to experimentalFasciola hepaticainfection in sheep

Cited by 37 publications

References 10 publications

Local hepatic immune response in rats during primary infection withFasciola hepatica

Local hepatic immune response in rats during primary infection withFasciola hepatica

Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

Early hepatic immune response in rats infected with Fasciola hepatica

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