Local induction of a cytotoxic factor in a murine tumour by systemic administration of an antitumour polysaccharide, MGA

Takahashi, Kazue; Watanuki, Yutaka; Yamazaki, Masatoshi; Abe, Shigeru

doi:10.1038/bjc.1988.35

Cited by 24 publications

(6 citation statements)

References 9 publications

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“…Otterlei et al (1991) showed that alginate with high M-and MG-blocks (isolated from Ascophyllum nodosum) were much more potent in inducing IL-1, IL-6, and TNF-α cytokine production compared with alginate with high G blocks (prepared from Laminaria digitata) [50]. Mannoglucan showed also TNF-α-like antitumor activity [51]. These results are in contrast with the anti-inflammatory activity of alginate demonstrated in our study.…”

Section: Discussioncontrasting

confidence: 80%

Extraction, Structural Characterization, and In Vivo Anti-Inflammatory Effect of Alginate from Cystoseira crinita (Desf.) Borry Harvested in the Bulgarian Black Sea

et al. 2023

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The aim of this study was to identify the chemical composition and sequential structure of alginate isolated from C. crinita harvested in the Bulgarian Black Sea, as well as its effects in histamine-induced paw inflammation in rats. The serum levels of TNF-α, IL-1β, IL-6, and IL-10 in rats with systemic inflammation, and the levels of TNF-α in a model of acute peritonitis in rats were also investigated. The structural characterization of the polysaccharide was obtained by FTIR, SEC-MALS, and 1H NMR. The extracted alginate had an M/G ratio of 1.018, a molecular weight of 7.31 × 104 g/mol, and a polydispersity index of 1.38. C. crinita alginate in doses of 25 and 100 mg/kg showed well-defined anti-inflammatory activity in the model of paw edema. A significant decrease in serum levels of IL-1β was observed only in animals treated with C. crinita alginate in a dose of 25 mg/kg bw. The concentrations of TNF-α and IL-6 in serum were significantly reduced in rats treated with both doses of the polysaccharide, but no statistical significance was observed in the levels of the anti-inflammatory cytokine IL-10. A single dose of alginate did not significantly alter the levels of the pro-inflammatory cytokine TNF-α in the peritoneal fluid of rats with a model of peritonitis.

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Section: Discussioncontrasting

confidence: 80%

Extraction, Structural Characterization, and In Vivo Anti-Inflammatory Effect of Alginate from Cystoseira crinita (Desf.) Borry Harvested in the Bulgarian Black Sea

et al. 2023

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“…We tested several preparations of AMPH (Sigma Chemical Co.) and AMPH (Fungizone; Bristol-Myers Squibb Co.) with similar results and observed that DMSO neither stimulated the production of TNF nor was a primer for TNF production (data not shown). Additionally, these TNF activities in the supematants of the macrophages stimulated by AMPH preparations and OK432 were immunologically confirmed to be neutralized by a rabbit antibody to TNF prepared as described previously (15). The doubt that the endotoxin that was possibly contaminated in the AMPH preparation might affect TNF production was ex- Endotoxin-insensitive macrophages from C3H/He J mice also produced TNF when stimulated by AMPH and OK432 (data not shown).…”

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confidence: 71%

Augmentation of murine tumor necrosis factor production by amphotericin B in vitro and in vivo

Tokuda

Tsuji

Yamazaki

et al. 1993

Antimicrob Agents Chemother

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Murine peritoneal macrophages were preincubated with amphotericin B (AMPH) and were then stimulated with bacterial lipopolysaccharide or streptococcal preparation (OK432). These macrophages produced a large amount of tumor necrosis factor. When administered to mice, the priming activity of amphotericin B for tumor necrosis factor production in vivo was also observed.Amphotericin B (AMPH) is known to activate macrophage functions in terms of oxidative burst (17) and fungicidal activity (10). It was also reported to induce the production of a marginal level of tumor necrosis factor-a (TNF) in murine macrophages in vitro (2, 3). There is much evidence that TNF exhibits many types of immunological properties (1,4,14). This suggests that some part of the immunological or pharmacological actions of AMPH may be mediated by TNF. Efficient induction of TNF by macrophages is known to require at least two types of stimulation (7). Here, we report that AMPH induces a relatively large amount of TNF in vitro and in vivo when combined with other macrophage stimulators.C3H/HeN mice were obtained from Shizuoka Experimental Animal Corporation, Hamamatsu, Japan. Deoxycholatefree AMPH supplied from Bristol-Myers Squibb K. K., Tokyo, Japan, was used unless indicated otherwise. AMPH was dissolved in dimethyl sulfoxide (DMSO), and final concentrations of DMSO in culture media were less than 0.05%. Endotoxin contamination of all tested AMPH preparations except AMPH as Fungizone (Bristol-Myers Squibb Co.) was estimated to be less than 15 pg/i ,ug of AMPH by a Limulus assay (Endospecy; Seikagaku Industrial Co., Tokyo, Japan). Bacterial lipopolysaccharide (LPS) from Escherichia coli 0127:B8 was purchased from Difco Laboratories. A streptococcal preparation, OK432, was kindly supplied by Chugai Chemical Co., Tokyo, Japan. Antitumor mannoglucan polyalcohol (MGA) was supplied by Daiichi Pharmaceutical Co., Ltd., Tokyo, Japan. Fetal calf serum, in which contaminating endotoxin was present at less than 3 pg/ml, was purchased from Hyclone Co., Logan, Utah.Male C3H/HeN mice (6 weeks old) were injected intraperitoneally with 3 ml of 3% thioglycolate. Four days later, peritoneal cells were collected, washed twice with RPMI 1640 medium containing 5% fetal calf serum, and adjusted to 1.0 x 106 cells per ml in the same medium. They were seeded into flat-bottom microtiter plates (2 x 105 cells per well) and incubated for 2 h in a 5% CO2 incubator at 37°C, and nonadherent cells were removed. The monolayers, which consisted of macrophages (>95%) (9), were incubated in the presence of several concentrations of AMPH for 1 h and were then restimulated by the addition of * Corresponding author. LPS or OK432 for an additional 2 h. The viabilities of the macrophages, which were checked by the trypan blue dye exclusion test, were always greater than 95% before stimulation, and AMPH at a concentration of less than 5 ,ug/ml did not affected viability. The culture supernatants of each well were collected and were used immediately for the TNF assay. The TNF activi...

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“…TNF-␣ induces lysis of malignant cells and causes regression of some animal tumors [52], so that one possible mechanism for the anti-tumoral effect of polysaccharides has been related to induction of TNF [53][54][55][56][57][58]. Now, TNF-␣ production induced by the acidic arabinogalactan ARAGAL (100 mg/kg), following treatment of animals, was increased 26-fold when compared with the control group.…”

Section: Discussionmentioning

confidence: 93%

Induction of secretory and tumoricidal activities in peritoneal macrophages activated by an acidic heteropolysaccharide (ARAGAL) from the gum of Anadenanthera colubrina (Angico branco)

et al. 2004

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Local induction of a cytotoxic factor in a murine tumour by systemic administration of an antitumour polysaccharide, MGA

Cited by 24 publications

References 9 publications

Extraction, Structural Characterization, and In Vivo Anti-Inflammatory Effect of Alginate from Cystoseira crinita (Desf.) Borry Harvested in the Bulgarian Black Sea

Extraction, Structural Characterization, and In Vivo Anti-Inflammatory Effect of Alginate from Cystoseira crinita (Desf.) Borry Harvested in the Bulgarian Black Sea

Augmentation of murine tumor necrosis factor production by amphotericin B in vitro and in vivo

Induction of secretory and tumoricidal activities in peritoneal macrophages activated by an acidic heteropolysaccharide (ARAGAL) from the gum of Anadenanthera colubrina (Angico branco)

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