Local Injection of Hyaluronic Acid Filler Improves Open Gingival Embrasure: Validation Through a Rat Model

Pi, Soojin; Choi, Yoon Jeong; Hwang, Soonshin; Lee, Dong-Won; Yook, Jong In; Kim, Kyung Ho; Chung, Chooryung J.

doi:10.1902/jop.2017.170101

Cited by 22 publications

(16 citation statements)

References 34 publications

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“…An alternative method for assessing the biological response and safety of an implanted material is an implantation in the dorsal subcutaneous tissue of mice, rats, guinea-pigs, or rabbits (Pi et al, 2017 ). For example, the implantation of films made of HA tested by subcutaneous implantation into the backs of Wistar rats (Liu et al, 2005 ).…”

Section: In Vivo Tests Of Ha-based Materialsmentioning

confidence: 99%

An Effective Translation: The Development of Hyaluronan-Based Medical Products From the Physicochemical, and Preclinical Aspects

Huerta-Ángeles

Nešporová

Ambrožová

et al. 2018

Front. Bioeng. Biotechnol.

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This review shows the steps toward material selection focalized on the design and development of medical devices based on hyaluronan (HA). The selection is based on chemical and mechanical properties, biocompatibility, sterilization, safety, and scale-up costs. These facts play a vital role in the industrialization process. Approved medical devices containing-HA are illustrated to identify key parameters. The first part of this work involves the steps toward a complete characterization of chemical and mechanical aspects, reproducibility of the processes and scale up. In a second stage, we aimed to describe the preclinical in vitro and in vivo assays and selected examples of clinical trials. Furthermore, it is important to keep in mind the regulatory affairs during the research and development (R&D) using standardization (ISO standards) to achieve the main goal, which is the functionality and safety of the final device. To keep reproducible experimental data to prepare an efficient master file for the device, based on quality and recorded manufacturing data, and a rigorous R&D process may help toward clinical translation. A strong debate is still going on because the denominated basic research in HA field does not pay attention to the purity and quality of the raw materials used during the development. So that, to achieve the next generation of devices is needed to overcome the limitations of state of art in terms of efficacy, biodegradability, and non-toxicity.

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Section: In Vivo Tests Of Ha-based Materialsmentioning

confidence: 99%

An Effective Translation: The Development of Hyaluronan-Based Medical Products From the Physicochemical, and Preclinical Aspects

Huerta-Ángeles

Nešporová

Ambrožová

et al. 2018

Front. Bioeng. Biotechnol.

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“…3 Hyaluronic acid filler has been shown to be able to fill open gingival embrasures in an in vivo model. 6 Most previous studies have not taken the complex implications of tooth movement into consideration when evaluating open gingival embrasures. Recent studies have reported that the movement of teeth during orthodontic treatment could also affect the occurrence of open gingival embrasures.…”

Section: Introductionmentioning

confidence: 99%

Risk factors associated with open gingival embrasures after orthodontic treatment

Choi

Kim

et al. 2018

The Angle Orthodontist

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Objectives: To investigate the incidence of and contributing factors to open gingival embrasures between the central incisors after orthodontic treatment. Materials and Methods: One hundred posttreatment patients (29 men and 71 women; mean age, 24.7 years) were divided retrospectively into occurrence and nonoccurrence groups based on intraoral photographs. Based on the severity, the occurrence group was further divided into mild, moderate, and severe groups. Parameters from periapical radiographs, superimposed lateral cephalograms, and study models were compared between the occurrence and the nonoccurrence groups by using independent t-tests and were also analyzed on the basis of severity via analysis of variance. Logistic regression analysis was performed to identify the contributing factors to open gingival embrasures. Results:The incidence of open gingival embrasures between the central incisors was 22% and 36% in the maxilla and the mandible, respectively. Lingual movement of the incisors, distance from the contact point to the alveolar crest after treatment, antero-posterior overlap of the two central incisors before treatment in the maxilla, and distance from the contact point to the alveolar crest after treatment in the mandible were significantly associated with the occurrence of open gingival embrasures (P , .05). In the mandible, the amount of intrusion was significantly related to severity (P , .05). Conclusions:The incidence of open gingival embrasures following orthodontic tooth movement is high. Therefore, attention should be paid to the contributing factors to prevent or reduce the occurrence of open gingival embrasures. (Angle Orthod. 2018;88:267-274.)

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“…A previous study successfully developed an experimentally induced OGE model using rats and demonstrated the effect of HA filler injection on IDP reconstruction. However, the study also suggested the need for a histological evaluation to examine the side effects of HA filler injection [34]. Based on the findings of a previous study, OGE was mimicked by inducing IDP loss between both incisors of mice in this study [34].…”

Section: Discussionmentioning

confidence: 78%

“…However, the study also suggested the need for a histological evaluation to examine the side effects of HA filler injection [34]. Based on the findings of a previous study, OGE was mimicked by inducing IDP loss between both incisors of mice in this study [34]. The morphological changes in IDP of the OGE mouse model were evaluated after wire attachment.…”

Section: Discussionmentioning

confidence: 97%

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Effect of Hyaluronic Acid Filler Injection on the Interdental Papilla in a Mouse Model of Open Gingival Embrasure

Kim

Cho

Jue

et al. 2020

IJERPH

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The black triangle resulting from interdental papilla (IDP) loss is associated with poor aesthetics and difficulty in pronunciation and food impaction. There is limited knowledge of gingival tissue inflammatory response to hyaluronic acid (HA) filler injection, a minimally invasive IDP reconstruction method. This study aimed to examine the morphological and histological changes in IDP and the inflammatory cytokine localization to the IDP post-HA filler injection using an open gingival embrasure (OGE) mouse model. Mice from the control, sham, and OGE groups were attached with reference, inactive, and activated wires for 5 days, respectively. The degree of IDP loss was determined based on the spring-papilla distance (SPD). Morphological and histological changes in the OGE group injected with phosphate-buffered saline (PBS) or HA fillers were examined on days 2 and 7 post-injection. Immunohistochemical analysis was performed to determine the localization patterns of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, myeloperoxidase (MPO), and Ki67. Five days post-wire attachment, the control and OGE groups exhibited a significantly higher SPD than the sham group (p < 0.0167). The SPD of the HA filler injection group was significantly lower than that of the PBS injection group on days 2, 4, and 7 post-injection (p < 0.05). The IDP of the OGE group was wide and flat. HA filler was stable in the connective tissue underlying the epithelial tissue even on day 7 post-injection. TNF-α, IL-1β, IL-6, MPO, and Ki67 were highly localized to the connective tissue surrounding the filler on day 2, which decreased on day 7 post-injection. Thus, HA filler can safely and successfully reconstruct the IDP in cases of OGE.

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Local Injection of Hyaluronic Acid Filler Improves Open Gingival Embrasure: Validation Through a Rat Model

Abstract: Open gingival embrasure was reproduced reliably in vivo. Local injection of HA filler was validated as a meaningful minimally invasive procedure to improve open gingival embrasure.

Cited by 22 publications

References 34 publications

An Effective Translation: The Development of Hyaluronan-Based Medical Products From the Physicochemical, and Preclinical Aspects

An Effective Translation: The Development of Hyaluronan-Based Medical Products From the Physicochemical, and Preclinical Aspects

Risk factors associated with open gingival embrasures after orthodontic treatment

Effect of Hyaluronic Acid Filler Injection on the Interdental Papilla in a Mouse Model of Open Gingival Embrasure

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