Peri-urban regions, especially in the Mediterranean, face challenges like farmland loss due to urban pressure. This study emphasizes retail stores as strategic focal points for evaluating societal, economic, and production systems. It hypothesizes that analyzing retail stores in agricultural areas provides insights into traded and cultivated agrobiodiversity. Using the Baix Llobregat Agrarian Park (Catalonia, NE Iberian Peninsula) as a case study, this research examines different food retailers from short and conventional food supply chains. Results indicate variations in plant diversity, origin, and seasonality among different retail stores. Farmers’ markets exhibit higher intraspecific diversity, contributing to local agrobiodiversity conservation. This study observes temporal changes in farmers’ markets, highlighting shifts influenced by socioeconomic factors and climate change perceptions. Finally, this research underscores certain strategies to promote sustainable peri-urban local food systems and preserve agrobiodiversity, offering valuable insights into food supply chain dynamics in peri-urban agricultural regions.