Local mechanical properties of mouse outer hair cells: Atomic force microscopic study

Murakoshi, Michio; Yoshida, Naohiro; Iida, Kei; Kumano, Shun; Kobayashi, Toshimitsu; Wada, Hiroshi

doi:10.1016/j.anl.2005.11.009

Cited by 19 publications

(12 citation statements)

References 31 publications

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“…3), which might reflect the sensory and supporting roles of these cell types in development. Although we directly probed the actin-rich cuticular plate at the OHC lumenal surface, we were unable to explore the basolateral actin content previously described in dissociated OHCs (Tolomeo and Holley, 1997;Murakoshi et al, 2006), which contributes to the heterogeneity in OHC Young's modulus between apical and basolateral areas at adult stages (Zelenskaya et al, 2005;Murakoshi et al, 2006). Instead, by making measurements within the semi-intact organ of Corti, we were able to describe cellspecific changes in surface mechanical properties of this tissue along OHC rows and not PC rows (Figs 4,5), which might inform future theoretical models of cochlear morphogenesis.…”

Section: Cytoskeletal Architecture Mediates Developing Mechanical Promentioning

confidence: 99%

“…1). Young's modulus, also referred to as stiffness, is a material property describing the resistance of structure to mechanical deformation, where an increasing Young's modulus indicates cell stiffening ( Murakoshi et al, 2006). As the structural organization of cells in the cochlea is specific to transmit mechanical sound stimuli, the measurement of the cell stiffness can lead to further understanding the important developmental events within the inner ear.…”

Section: Introductionmentioning

confidence: 99%

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Cytoskeletal changes in actin and microtubules underlie the developing surface mechanical properties of sensory and supporting cells in the mouse cochlea

et al. 2012

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SUMMARYCorrect patterning of the inner ear sensory epithelium is essential for the conversion of sound waves into auditory stimuli. Although much is known about the impact of the developing cytoskeleton on cellular growth and cell shape, considerably less is known about the role of cytoskeletal structures on cell surface mechanical properties. In this study, atomic force microscopy (AFM) was combined with fluorescence imaging to show that developing inner ear hair cells and supporting cells have different cell surface mechanical properties with different developmental time courses. We also explored the cytoskeletal organization of developing sensory and non-sensory cells, and used pharmacological modulation of cytoskeletal elements to show that the developmental increase of hair cell stiffness is a direct result of actin filaments, whereas the development of supporting cell surface mechanical properties depends on the extent of microtubule acetylation. Finally, this study found that the fibroblast growth factor signaling pathway is necessary for the developmental time course of cell surface mechanical properties, in part owing to the effects on microtubule structure.

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Section: Cytoskeletal Architecture Mediates Developing Mechanical Promentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytoskeletal changes in actin and microtubules underlie the developing surface mechanical properties of sensory and supporting cells in the mouse cochlea

et al. 2012

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“…20 Regarding cells, E Y of human umbilical vein endothelial cells (HUVECs) with regional variations ranged from 1 to 7 kPa, 14 and E Y of outer hair cells from the cochlea of mice was ~2 kPa. 21 The age-dependent apparent elastic moduli of cardiac myocytes were in the range of 35 kPa to 43 kPa, 22 and the values of human aortic endothelial cells (HAECs) were ~1.5 kPa and ~5.6 kPa for two distinct populations. 23 In conjunction with Hertzian analysis, AFM indentation measurements with sharp-conical tips were also performed to evaluate E Y of melanosomes in the retinal pigment epithelium (~100 MPa) and E Y of escherichia coli (~182 MPa, ~13 MPa, and ~1 MPa in methanol, water, and formamide, respectively).…”

Section: Introductionmentioning

confidence: 99%

Effect of tip geometry of atomic force microscope on mechanical responses of bovine articular cartilage and agarose gel

Park

Duong

Lee

et al. 2010

Int. J. Precis. Eng. Manuf.

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The objective of this study is to investigate the effect of different Atomic Force Microscope (AFM) tip geometries (sharp-conical and spherical tips) on the microscale Young's modulus of bovine articular cartilage and agarose gel that is calculated by the method of the average point-wise modulus. The measurements of the microscale Young's moduli of 3% agarose gel under a conical AFM tip (20.9±4.9 kPa) and under a spherical AFM tip (17.5±3.0 kPa), averaged over an indentation depth of 600 nm, were comparable. However, the microscale Young's moduli of articular cartilage, as measured with a conical AFM tip (116.9±62.9 kPa), were significantly higher than the corresponding values under a spherical AFM tip (30.9±14.3 kPa). The results of the current study suggest that the AFM tip geometry affects the microscale measurements of the mechanical properties on the surfaces of biological materials. The findings of the study can help to elucidate more accurately the microscale mechanical properties on the surface layers of diverse biological materials including tissue-engineered cartilages with different material characteristics.

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“…AFM can be useful for studying cell pathology Simon and Durrieu, 2006;Sirghi and Rossi, 2006;Sirghi et al, 2008;Guzmá n et al, 2006;Radmacher, 2007;Rosenbluth et al, 2006;Lam et al, 2007;Murakoshi et al, 2006;Fitzgerald, 2006;Crick and Yin, 2007;Kuznetsova et al, 2007;Orr et al, 2006;Costa, 2006;Addae-Mensah and Wikswo, 2008). Any changes in cell structures can cause the alterations in mechanical properties of cell (Lee and Lim, 2007).…”

Section: Introductionmentioning

confidence: 99%

Connection between biomechanics and cytoskeleton structure of lymphocyte and Jurkat cells: An AFM study

Cai

Xing

Cai

et al. 2010

Micron

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Local mechanical properties of mouse outer hair cells: Atomic force microscopic study

Cited by 19 publications

References 31 publications

Cytoskeletal changes in actin and microtubules underlie the developing surface mechanical properties of sensory and supporting cells in the mouse cochlea

Cytoskeletal changes in actin and microtubules underlie the developing surface mechanical properties of sensory and supporting cells in the mouse cochlea

Effect of tip geometry of atomic force microscope on mechanical responses of bovine articular cartilage and agarose gel

Connection between biomechanics and cytoskeleton structure of lymphocyte and Jurkat cells: An AFM study

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