Localisation of the benzimidazole fungicide binding site of <i>Gibberella zeae</i> β<sub>2</sub>‐tubulin studied by site‐directed mutagenesis

Qiu, Jianbo; Xu, Jianqiang; Yu, Junjie; Bi, Chaowei; Chen, Changjun; Zhou, Mingguo

doi:10.1002/ps.2050

Cited by 82 publications

(67 citation statements)

References 24 publications

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“…The sensitivity of all strains to carbendazim was measured as described [69]. Sensitive strains could not grow on PDA plates containing 1.4 μg/mL carbendazim, while resistant strains grew normally on this medium.…”

Section: Methodsmentioning

confidence: 99%

Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China

Qiu

Shi

2014

Toxins

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Members of the Fusarium graminearum species complex (FGSC) are important pathogens on wheat, maize, barley, and rice in China. Harvested grains are often contaminated by mycotoxins, such as the trichothecene nivalenol (NIV) and deoxynivalenol (DON) and the estrogenic mycotoxin zearalenone (ZEN), which is a big threat to humans and animals. In this study, 97 isolates were collected from maize, wheat, and rice in Jiangsu and Anhui provinces in 2013 and characterized by species- and chemotype-specific PCR. F. graminearum sensu stricto (s. str.) was predominant on maize, while most of the isolates collected from rice and wheat were identified as F. asiaticum. Fusarium isolates from three hosts varied in trichothecene chemotypes. The 3-acetyldeoxynivalenol (3ADON) chemotype predominated on wheat and rice population, while 15ADON was prevailing in the remaining isolates. Sequence analysis of the translation elongation factor 1α and trichodiene synthase indicated the accuracy of the above conclusion. Additionally, phylogenetic analysis suggested four groups with strong correlation with species, chemotype, and host. These isolates were also evaluated for their sensitivity to carbendazim and mycotoxins production. The maize population was less sensitive than the other two. The DON levels were similar in three populations, while those isolates on maize produced more ZEN. More DON was produced in carbendazim resistant strains than sensitive ones, but it seemed that carbendazim resistance had no effect on ZEN production in wheat culture.

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Section: Methodsmentioning

confidence: 99%

Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China

Qiu

Shi

2014

Toxins

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“…The resistance to MBC fungicides is caused by point mutations in the b2-tubulin [19,20]. Deletion of the b2-tubulin resulted in reduced conidiation, vegetative growth and pathogenicity, but increased sensitivity to carbendazim [19,21]. In addition, the b1-tubulin deletion mutants displayed also reduced vegetative growth and pathogenicity, produced shorten spores, and were resistant to carbendazim [22], indicating both b1-and b2-tubulins play important roles in vegetative growth, conidiation, and responses to fungicides in F. asiaticum.…”

Section: Introductionmentioning

confidence: 99%

α1-Tubulin FaTuA1 plays crucial roles in vegetative growth and conidiation in Fusarium asiaticum

Zhang

Chen

et al. 2015

Research in Microbiology

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“…In fact, many mutation sites at the β-tubulin locus have been found in benzimidazole-resistant plant-pathogenic fungi (e.g., Albertini et al 1999;Qiu et al 2011), but most molecular studies have focused on replacement of the amino acid at position 198 and/or 200 (e.g., Davidson et al 2006;Fujimura et al 1992;Hollomon et al 1998). …”

Section: Resultsmentioning

confidence: 99%

Benomyl-resistant Beauveria bassiana (Hypocreales: Clavicipitaceae) mutants induced by ion beams

et al. 2014

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Fungicides targeted at phytopathogens can be harmful to entomopathogenic fungi. The purpose of this study was to use ion-beam irradiation technology to produce benomyl-resistant mutants of the entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuill. After irradiation of conidia at 150 Gy, two mutant isolates, BB22 and BB24, were selected on media containing the fungicide. In an assay of vegetative growth, BB22 and BB24 were over 500 and 800 times more tolerant to benomyl, respectively, compared with the wild isolate. However, in an assay of conidial germination, neither mutant had increased tolerance compared with the wild isolate. Both mutant isolates also had increased tolerance to thiophanate-methyl during vegetative growth, but reduced tolerance to diethofencarb. A mutation was found at position 198 of the β-tubulin gene in the mutant isolates, with a substitution of glutamate for alanine (E198A). Ion beams have great potential as a tool to improve the traits of entomopathogenic fungi such as increasing tolerance to fungicides. Fungicide-resistant mutants produced in this way could be useful agents for biological control within IPM programmes where fungicides are also used.

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Localisation of the benzimidazole fungicide binding site of Gibberella zeae β₂‐tubulin studied by site‐directed mutagenesis

Abstract: Mutations at codons 50, 167, 198 and 200 of G. zeae β(2)tub could cause resistance to carbendazim, and these codons may form a binding pocket.

Cited by 82 publications

References 24 publications

Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China

Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China

α1-Tubulin FaTuA1 plays crucial roles in vegetative growth and conidiation in Fusarium asiaticum

Benomyl-resistant Beauveria bassiana (Hypocreales: Clavicipitaceae) mutants induced by ion beams

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Localisation of the benzimidazole fungicide binding site of Gibberella zeae β2‐tubulin studied by site‐directed mutagenesis

Abstract: Mutations at codons 50, 167, 198 and 200 of G. zeae β(2)tub could cause resistance to carbendazim, and these codons may form a binding pocket.

Cited by 82 publications

References 24 publications

Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China

Genetic Relationships, Carbendazim Sensitivity and Mycotoxin Production of the Fusarium Graminearum Populations from Maize, Wheat and Rice in Eastern China

α1-Tubulin FaTuA1 plays crucial roles in vegetative growth and conidiation in Fusarium asiaticum

Benomyl-resistant Beauveria bassiana (Hypocreales: Clavicipitaceae) mutants induced by ion beams

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Localisation of the benzimidazole fungicide binding site of Gibberella zeae β₂‐tubulin studied by site‐directed mutagenesis