Localisations histochimiques.: II: Procédés simples de localisation de pigments flavoniques. Application à quelques Phanérogames

Guérin, Hélène; Delaveau, Pierre; Paris, Renzo

doi:10.1080/00378941.1971.10838874

Cited by 60 publications

(32 citation statements)

References 2 publications

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“…For histochemical tests, cross sections of fresh fronds of Doryopteris triphylla were made and the following reagents were applied: Toluidine blue to detect polysaccharides [30], Nile Blue for neutral and acidic lipids [31], Ferrum chloride for dihydroxyphenols (catechol phenols) [32], Phloroglucinol stain for lignin [31], Neutral red for lipids [33], Ruthenium red for non-cellulose polysaccharides such as pectin [31], Sudan IV for lipids [31] [35], Vanillin/HCl [34] and Aluminum trichloride [34] under UV for flavonoids.…”

Section: Histochemical Testsmentioning

confidence: 99%

Anatomical, Histochemical and Cytogenetic Features of <i>Doryopteris triphylla</i> (Pteridaceae)

Neira¹,

Andrada²,

Páez³

et al. 2017

AJPS

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Doryopteris triphylla (Pteridaceae-Cheilanthoideae) grows in xeric habitats in Brazil, Paraguay, Uruguay and Argentina. The aim of this study was to characterize D. triphylla anatomically, histochemically and cytogenetically. For anatomical characterization, rhizomes, roots, petioles and leaves were made and then stained using Safranine-Astra Blue for further observations. Leaf blades were also cleared. For histochemical analysis, leaf cross sections were stained with different reagents to identify glandular trichomes compounds. For cytogenetic characterization, a karyogram was performed using laboratory cultivated roots. Results show a dictyostelic rhizome covered with scales with apical secreting gland; diarch roots; petiole cross-sections show thick cuticle, uniseriate epidermis, parenchymatic cortex cells with thick walls and a vascular bundle with two xylem groups; and hypostomatic fronds with glandular trichomes. Histochemical studies of secretion products of the glandular trichomes were positive for polysaccharides, pectins, lipids, acid lipids, dihydroxyphenols, phenols and flavonoids. Cytogenetically, D. triphylla is described as a diploid species (2n = 60), with chromosomes gradually decreasing in size. The apical glands in scales of rhizomes, the presence of two xylem groups in the vascular bundle in the petiole and the glandular trichomes on the abaxial surface are new contributions to the species. The type of chemical products secreted by glandular leaf trichomes and karyotype estimation is shown for the first time in this species.

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Section: Histochemical Testsmentioning

confidence: 99%

Anatomical, Histochemical and Cytogenetic Features of <i>Doryopteris triphylla</i> (Pteridaceae)

Neira¹,

Andrada²,

Páez³

et al. 2017

AJPS

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“…For light microscopy, free hand sections were stained with a 0.5% toluidine blue (TBO) solution, which stains acidic carbohydrates blue and phenolic compounds green (Egerton-Warburton 1998). FeCl 3 solution (1%) was used for O-dihydroxyphenols that stained dark brown or dark blue (Guerin et al 1971). Stained sections were then observed under light microscope (type Olympus DX41).…”

Section: Methodsmentioning

confidence: 99%

Histochemical Localization of Essential Oils and Bioactive Substances in the Seed Coat of the Halophyte Crithmum maritimum L. (Apiaceae)

et al. 2009

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Despite seeds and fruits of several halophytes being rich in essential oils and other bioactive substances, the histochemical characterization of these compounds has not received much attention. The aims of the present investigation were to localize the essential oils and the bioactive substances in the seed coat of the oilseed halophyte Crithmum maritimum L. Fruits were collected from the rocky coasts of Tabarka (NW of Tunisia, 36°57′ 12″ N, 08°45′18″ E). C. maritimum L. seed is shown to be surrounded by two envelopes: The first structure is a secretory envelope, consisting in about 20 canals. The second layer represents the endocarp. As revealed by fluorescence and light microscopy, the essential oils, O-dihydroxyphenols and flavonoids, known as bioactive compounds, were accumulated in the canals. The endocarp layer accumulated polysaccharides, O-dihydroxyphenols, and flavonoids. As a whole, these findings highlight the histochemical features and confirm the valuable quality of C. maritimum L. seeds.

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“…For light microscopy and histochemistry (bright field and fluorescence), transverse sections of approximately 25 μm thickness were cut with a cryotome and stained with Nadi reagent for the detection of phenolics, Sudan IV (Jensen, 1962) for lipids, Dragendorff reagent for alkaloids, and AlCl 3 (Guerin et al, 1971) for the enhancement of fluorescence of flavonoids. The samles were observed and photographed with an Olympus BX40 light microscope, equipped with an Olympus DP21 digital camera.…”

Section: Methodsmentioning

confidence: 99%

Limoniastrum monopetalum (L.) Boiss, a Candidate plant for use in Urban and suburban Areas with Adverse Conditions. An Anatomical and Histochemical Study.

Ioannidou¹,

Spentza²,

Fasseas³

2015

BUASVMCN-HORT

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Limoniastrum monopetalum L. is an interesting small shrub native to Greece and other Mediterranean countries with an attractive foliage, and blooming in the summer with pink flowers. In this work an anatomical and hystochemical study of the species were carried out in order to investigate the suitability of the L. monopetalum for use in urban and suburban areas as ornamental plant and its growth in hostile environments such as archeological sites, roof gardens, landscape restoration (e.g. quarries, roadsides, etc.). The results showed that the leaves of L. monopetalum are amphistomatic bifacial with leaf blade approx. 170 μm thick. The epidermal cells is relatively thick approximately 20 μm and covered by a 2 μm thick cuticle. Furthermore both surfaces of the leaves are entirely covered by salt crystals secreted by the salt glands, as this is revealed from fresh unprocessed specimens observed with the SEM.The histochemical tests showed the presence of terpenoids in the cell walls of the epidermal cells and in the leaf vascular tissues, phenolics, nitrites and alkaloids, flavonoids in the mesophyll cells, especially in the vacuoles, and lipids only in the cuticle. Both morphological anatomical and histochemical characteristics of the leaves indicate the xeromorphic character of the species which render L. monopetalum a suitable plant for ornamental use in a wide spectrum of areas with adverse conditions

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Localisations histochimiques.: II: Procédés simples de localisation de pigments flavoniques. Application à quelques Phanérogames

Cited by 60 publications

References 2 publications

Anatomical, Histochemical and Cytogenetic Features of <i>Doryopteris triphylla</i> (Pteridaceae)

Anatomical, Histochemical and Cytogenetic Features of <i>Doryopteris triphylla</i> (Pteridaceae)

Histochemical Localization of Essential Oils and Bioactive Substances in the Seed Coat of the Halophyte Crithmum maritimum L. (Apiaceae)

Limoniastrum monopetalum (L.) Boiss, a Candidate plant for use in Urban and suburban Areas with Adverse Conditions. An Anatomical and Histochemical Study.

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Localisations histochimiques.: II: Procédés simples de localisation de pigments flavoniques. Application à quelques Phanérogames

Cited by 60 publications

References 2 publications

Anatomical, Histochemical and Cytogenetic Features of &lt;i&gt;Doryopteris triphylla&lt;/i&gt; (Pteridaceae)

Anatomical, Histochemical and Cytogenetic Features of &lt;i&gt;Doryopteris triphylla&lt;/i&gt; (Pteridaceae)

Histochemical Localization of Essential Oils and Bioactive Substances in the Seed Coat of the Halophyte Crithmum maritimum L. (Apiaceae)

Limoniastrum monopetalum (L.) Boiss, a Candidate plant for use in Urban and suburban Areas with Adverse Conditions. An Anatomical and Histochemical Study.

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Anatomical, Histochemical and Cytogenetic Features of <i>Doryopteris triphylla</i> (Pteridaceae)

Anatomical, Histochemical and Cytogenetic Features of <i>Doryopteris triphylla</i> (Pteridaceae)