Localization and partial characterization of a rat ovarian granulose cell protein with a monoclonal antibody

Russinova, Angelina; Vassilev, Alex; Davidoff, M

doi:10.1016/0248-4900(93)90145-5

Cited by 4 publications

(2 citation statements)

References 29 publications

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“…Further controls were performed with subclass-matched control Mab 5G5 (IgG) obtained against rat ovarian antigens, produced and characterized in our laboratory after immunization with granulosa cells and oocytes (Russinova et al, 1993(Russinova et al, , 1994). An additional control was performed with Mab 2F2 preabsorbed with protein extract obtained from the cells utilized for immunization before being processed for immunohistochemistry.…”

Section: Controls For Light Microscope Observationmentioning

confidence: 99%

Stage-specific nuclear antigen is expressed in rat male germ cells during early meiotic prophase

et al. 2000

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Section: Controls For Light Microscope Observationmentioning

confidence: 99%

Stage-specific nuclear antigen is expressed in rat male germ cells during early meiotic prophase

et al. 2000

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“…In controls, the primary antibody was replaced with (i) normal mouse serum; (ii) Mab 6C3 absorbed with synthetic ANF (20 µg ml -1 ) for 24 h at 4°C or (iii) PBS instead of Mab 6C3. Further controls were performed with Mab 5G5 (IgG) obtained against rat ovarian antigen which bound a 59-kDa protein located on granulosa and Leydig cell surface (Russinova et al, 1993;Russinova et al, 1995) as a subclass matched control.…”

Section: Immunocytochemical Controlsmentioning

confidence: 99%

Immunocytochemical localization of atrial natriuretic factor (ANF) in rat female reproductive tract: evidence for a potential hormonal regulation

Russinova

Mourdjeva

Valkova

et al. 2002

Biology of the Cell

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In the present studies atrial natriuretic factor (ANF) was characterized immunocytochemically in the reproductive tract of immature female rats, and changes of ANF levels in response to different hormonal conditions were demonstrated. Administration of pregnant mare serum gonadotropin (PMSG) to immature animals has shown to be a useful method to synchronize growth, differentiation and atresia of ovarian follicles. ANF immunoreactivity was investigated in rat uterus and oviduct during follicular growth and estrogenic dominance (48 h after PMSG treatment) and during follicular atresia and progesterone dominance (96 h after PMSG treatment). Our immunocytochemical results showed that in rat uterus ANF was localized in endometrial mucosal and glandular epithelium and smooth muscle cells of the myometrium. In the oviduct ANF immunoreactivity was observed in mucosal cells and muscle layers. Immunocytochemical staining patterns and Western blot analysis revealed that ANF levels in rat uterus and oviduct are modulated by the hormonal status. ANF immunoreactivity was elevated during estrogenic dominance (48 h after PMSG) in uterus and oviduct. However, during progesterone dominance (96 h after PMSG) elevation of ANF immunoreactivity was observed in the uterus only. These results raise the possibility that ANF expression in rat oviduct is positively controlled by estrogen and negatively by progesterone. ANF staining in uterus during progesterone phase provides evidence that both estrogen and progesterone regulate ANF levels in uterus. The observed staining patterns indicate that ANF may have intracellular functions as well as a role in priming the extracellular environment. Accordingly, the possibility that ANF might be an important regulatory molecule for autocrine/paracrine communication within the female reproductive tract should be considered.

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Comparative investigation of the use of various commercial microcarriers as a substrate for culturing mammalian cells

Ayyildiz-Tamis

Avcı

Deliloglu-Gurhan

2013

In Vitro Cell.Dev.Biol.-Animal

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Microcarriers provide large adhesion area allowing high cell densities in bioreactor systems. This study focused on the investigation of cell adhesion and cell growth characteristics of both anchorage-dependent CHO-K1 and anchorage-independent Ag8 myeloma cell lines cultivated on four different microcarriers (Biosilon®, Microhex®, Cytodex 3®, Cytoline 2®) by considering the cell kinetics and physiological data. Experiments were performed in both static and agitated cell culture systems by using 24-well tissue culture plates and then 50-ml spinner flasks. In agitated cultures, the highest specific growth rates (0.026 h for CHO-K1 and 0.061 h for Ag8 cell line) were obtained with Cytodex 3® and Cytoline 2® microcarriers for CHO-K1 and Ag8 cell line, respectively. Metabolic characteristics showed some variation among the cultures with the four microcarriers. The most significant being the higher production of lactate with microcarriers with CHO-K1 cells relative to the Ag8 cells. SEM analyses revealed the differences in the morphology of the cells along with microcarriers. On Cytodex 3® and Cytoline 2®, CHO-K1 cells attached to the substratum through long, slender filopodia, whereas the cells showed a flat morphology by covering the substratum on the Biosilon® and Microhex®. Ag8 cells maintained their spherical shapes throughout the culture for all types of microcarriers. In an attempt to scale-up, productions were carried out in 50-ml spinner flasks. Cytodex 3® (for CHO-K1 cells) and Cytoline 2® (for Ag8 cells) were evaluated. The results demonstrate that high yield of biomass could be achieved through the immobilization of the cells in each culture system. And cell cultures on microcarriers, especially on Cytodex 3® and Cytoline 2®, represented a good potential as microcarriers for larger scale cultures of CHO-K1 and Ag8, respectively. Moreover, owing to the fact that the cell lines and culture media are specific, outcomes will be applicable for other clones derived from the same host cell lines.

show abstract

Localization and partial characterization of a rat ovarian granulose cell protein with a monoclonal antibody

Cited by 4 publications

References 29 publications

Stage-specific nuclear antigen is expressed in rat male germ cells during early meiotic prophase

Stage-specific nuclear antigen is expressed in rat male germ cells during early meiotic prophase

Immunocytochemical localization of atrial natriuretic factor (ANF) in rat female reproductive tract: evidence for a potential hormonal regulation

Comparative investigation of the use of various commercial microcarriers as a substrate for culturing mammalian cells

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