Localization and regulation of aquaporins in the murine larynx

Abstract

AQP1, 4, and 5 were expressed in the laryngeal epithelium including the vocal folds. Vocal fold AQP expression was minimal at the vocal process. AQP2, 3, 5, 6, 7, and 8 were localized to the submucosal glands. Expression of AQPs in the vagotomized region was significantly decreased compared with the contralateral intact side. The expression in the laryngeal glands was also decreased in the aged mice.

A Comparison of the Localization of Integral Membrane Proteins in Human and Rabbit Vocal Folds

“…Between species, the localization of AQP4 was most comparable in the VF epithelium, which is also supported by findings in mouse VFs 13 and studies in airway epithelium of rats. 22 Although there are few studies claiming a minimal role of AQP4 in transepithelial water transport in airway tissue, 20,23 AQP4 is believed to contribute to neural signaling 24 and cell migration 25 in glial cells.…”

“…The integral membrane transport protein AQP1 was primarily localized superficial LP in both human and rabbit VFs, which is supported by findings of AQP1 in mouse VFs. 13 Aquaporin 1 may serve an important role in vascular permeability of the VF LP. In corneal and respiratory tissue in mice, the deletion of this gene reduces fluid production in the eyes 19 and fluid movement across the endothelium that lines blood vessels in the lungs.…”

“…11 Aquaporins, (AQP), which are integral membrane protein that forms pores in the membranes of cells, facilitates the transport of water between cells and has been explored primarily in sheep 12 and mouse models. 13 Cell-cell adhesion and tight junction molecules such as E-cadherin, ZO-1, and occludin, have been more extensively studied across various animal species and humans, but the localization of this integral cell adhesion protein in animals has not been directly compared to humans. [14][15][16] Although the aforementioned integral membrane proteins have been explored in various animal models, the identification and localization of these proteins has not been localized in rabbit and compared to human VFs.…”

The Effect of Hydration on Voice Quality in Adults: A Systematic Review

“…The vocal folds are composed of layers of ligaments, muscles and soft tissue and within them is a highly hydrated interstitial tissue. Outwardly, they are covered by a thin layer of liquid (Ahmed, et al, 2012). As air is pushed through them, the resultant consequence is their vibration.…”

Impact of Irradiation on laryngeal hydration and lubrication in rat larynx

“…Many mammalian AQPs participate in water‐selective transportation across epithelial membranes . The expression profile of AQP families in animal VFs has been investigated elsewhere; however, correlation of VF liquid homeostasis with radiation‐induced dysphonia has not been established. In this study, the expression of AQP1, 4, and 5 in VF mucosa and AQP5 on the apical surface of laryngeal glands decreased in response to irradiation in vivo.…”