Localization of blood group antigens in human pancreas with lectin-horseradish peroxidase conjugates.

Ito, Nobuaki; Nishi, Katsuji; Nakajima, Mitsuru; Matsuda, Yoshie; Ishitani, Akiko; J, Mizumoto; Hirota, Tadaomi

doi:10.1267/ahc.19.205

Cited by 25 publications

(8 citation statements)

References 32 publications

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“…To mention one essential technical detail, normal serum used to block non-specific binding sites should be preabsorbed with neuraminidase-treated red blood cells in order to avoid anti-TF auto-antibody interference (Springer and Desai 1985). Another point to consider is the observed reactivity of pancreatic acinar cells and gastric mucins from non-secretors with PNA, but not from secretors (Ito et al 1986;Macartney 1986). In general terms, we would like to stress that the combined use of different mAbs and their side-by-side comparison, if necessary in combination with specific glycosidase digestion procedures, are of great importance in obtaining more accurate results.…”

Section: Discussionmentioning

confidence: 99%

Thomsen-Friedenreich-related carbohydrate antigens in normal adult human tissues: a systematic and comparative study

Cao

Stosiek

Springer

et al. 1996

Histochem Cell Biol

142

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A broad variety of normal human tissues were examined for the expression of Thomsen-Friedenreich (TF)-related histo-blood group antigens, TF (Gal beta 1-3GalNAc alpha 1-R), Tn (TF precursor, GalNAc alpha 1-R), sialosyl-Tn (NeuAc alpha 2-6GalNAc alpha 1-R), considered to be useful in cancer diagnosis and immunotherapy, and sialosyl-TF, the cryptic form of TF. These antigens or, more correctly, glycotopes, were determined by immunohistochemistry with at least two monoclonal antibodies (mAbs) each (except sialosyl-TF) as well as by lectin histochemistry. For a better dissection of sialosyl-TF and TF glycotopes, tissue sections were pretreated with galactose oxidase or the galactose oxidase-Schiff sequence. Staining with mAbs appeared to be more restricted than with the lectins used. Distribution patterns among normal epithelia were different for all four antigens. These antigens were also detected in some non-epithelial tissues. They can be classified in the following sequence according to the frequency of their occurrence in normal tissues: sialosyl-TF > > sialosyl-Tn > Tn > TF. Most of the positively staining sites for TF, Tn, and sialosyl-Tn are located in immunologically privileged areas. The complex results obtained with anti-TF mAbs (after treatment of the tissue sections with sialidase from Vibrio cholerae) and the lectins amaranthin and jacalin revealed a differential distribution of the subtypes of sialosyl-TF [NeuAc alpha 2-3Gal beta 1-3GalNAc alpha 1-R and Gal beta 1-3 (NeuAc alpha 2-6)GalNAc alpha 1-R] in normal human tissues. From our data it can be inferred that TF, Tn, and sialosyl-Tn are promising targets for a cancer vaccine.

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Section: Discussionmentioning

confidence: 99%

Thomsen-Friedenreich-related carbohydrate antigens in normal adult human tissues: a systematic and comparative study

Cao

Stosiek

Springer

et al. 1996

Histochem Cell Biol

142

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“…A glycosylation dependent on blood group systems was only analyzed by histochemical methods in secretory cell types so far: in the human submandibular gland (Ito et al, 1989;Nakayima et al, 1988) and in the pancreas (Ito et al, 1986(Ito et al, & 1990. Since the other cell types of the respiratory surface epithelium-except the secretory goblet cell-do not exhibit such carbohydrate components, it seems likely that the marked substances are a characteristical feature of the status of the basal cells.…”

Section: Discussionmentioning

confidence: 99%

Lectins and antibodies to blood group antigens as markers for the basal cells of the human respiratory epithelium

Bals¹,

Welsch

1997

Microsc. Res. Tech.

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“…Tissues were fixed in 10% formalin for 6-10 days and embedded in paraffin: serial sections were cut at a thickness of 4 l.tm. The blood groups (ABO and Lewis) of the tissue donors were determined as reported previously (Ito et al, 1986(Ito et al, , 1987. The secretor status of the donors was determined by the Lewis blood type or was deduced histochemically from the presence (secretor) or absence (non-secretor) of H antigens in serous cells of the corresponding donor's submandibular glands (Ito et al, 1989a).…”

Section: Methodsmentioning

confidence: 99%

“…Details of the labelled lectin staining methods have been reported previously (Ito et al, 1986). In brief, sections were incubated for 2 h at 4~ in a 20 gg ml -~ solution of lectin conjugate in 0.1 M phosphate buffer (pH 7.2) containing 0.2 M NaC1 and Img ml -~ bovine serum albumin.…”

Section: Methodsmentioning

confidence: 99%

Localization of blood-group-related linear poly-N-acetyllactosamine structure in different human tissues by Griffonia simplicifolia agglutinin-II staining following endo-?-galactosidase digestion

et al. 1994

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Endo-beta-galactosidase from Escherichia freundii cleaves polylactosaminyl structures as follows: R-GlcNAc beta I-3Gal beta I-4GlcNac beta I-R' + H2O-->R-GlcNAc beta I-3Gal + GlcNAc beta I-R'. By staining with Griffonia simplicifolia agglutinin-II following the enzyme digestion, the distribution of R-GlcNAc beta I-3Gal beta I-4GlcNAc can be demonstrated in tissue sections. This carbohydrate chain is one of the backbone structures carrying the blood-group-related antigens and, thus, localization of this structure may provide detailed information about the distribution of variants with different backbone structures. Various formalin-fixed, paraffin-embedded tissue sections were stained by Griffonia simplicifolia agglutinin-II with or without prior enzyme digestion and the reactivity of the agglutinin imparted by enzyme digestion was studied in the following tissues and cells: pancreatic acinar cells, gastric surface mucosae, duct cells and mucous cells of salivary glands and tracheal glands, surface epithelium of trachea, goblet cells of large intestine, columnar epithelium of uterine cervical glands, distal and collecting tubules of kidney, certain cells of anterior lobe and colloid of middle lobe of pituitary glands, epithelial reticular cells and Hassall's corpuscles of thymus and Kupffer cells of liver. In gastric surface mucosae, the reactivity of the agglutinin appeared in non-secretor individuals but not in the secretor individuals, and in mucous cells of salivary and tracheal glands the reactivity appeared in Le(a- b-) non-secretor individuals but not in Le(a + b-) non-secretor or secretor individuals.(ABSTRACT TRUNCATED AT 250 WORDS)

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Localization of blood group antigens in human pancreas with lectin-horseradish peroxidase conjugates.

Cited by 25 publications

References 32 publications

Thomsen-Friedenreich-related carbohydrate antigens in normal adult human tissues: a systematic and comparative study

Thomsen-Friedenreich-related carbohydrate antigens in normal adult human tissues: a systematic and comparative study

Lectins and antibodies to blood group antigens as markers for the basal cells of the human respiratory epithelium

Localization of blood-group-related linear poly-N-acetyllactosamine structure in different human tissues by Griffonia simplicifolia agglutinin-II staining following endo-?-galactosidase digestion

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