Localization of chondrocyte precursors in periosteum

Ito, Yoichi M.; Fitzsimmons, James S.; Sanyal, Abhik Kumar; Mello, María Alice Rostom de; Mukherjee, N.; O’Driscoll, Shawn W.

doi:10.1053/joca.2000.0378

Cited by 119 publications

(91 citation statements)

References 38 publications

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“…Periosteum has two discrete layers: an outer fibrous layer and an inner juxtaosseous cambial layer [21]. The fibrous layer appears to be composed of fibroblastic cells in a collagen and elastin fiber matrix along with a nerve and microvascular network [9,19].…”

Section: Introductionmentioning

confidence: 99%

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Ball

Bonzani

Bovis

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Periosteal cells are important in embryogenesis, fracture healing, and cartilage repair and could provide cells for osteochondral tissue engineering. Questions/purpose We determined whether a population of cells isolated from human periosteal tissue contains cells with a mesenchymal stem cell (MSC) phenotype and whether these cells can be expanded in culture and used to form tissue in vitro. Methods We obtained periosteal tissue from six patients. Initial expression of cell surface markers was assessed using flow cytometry. Cells were cultured over 10 generations and changes in gene expression evaluated to assess phenotypic stability. Phenotype was confirmed using flow cytometry and colony-forming ability assays. Mineral formation was assessed by culturing Stro-1 À and unsorted cells with osteogenic supplements. Three cell culture samples were used for a reverse transcription-polymerase chain reaction, four for flow cytometry, three for colony-forming assay, and three for mineralization. Results Primary cultures, containing large numbers of hematopoietic cells were replaced initially by Stro-1 and ALP-expressing immature osteoblastic cell types and later by ALP-expressing cells, which lacked Stro-1 and which became the predominant cell population during subculture.

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Section: Introductionmentioning

confidence: 99%

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Ball

Bonzani

Bovis

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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“…This potential of new bone formation is stimulated by infection, trauma, and growing lesions. The osteogenic potential of periosteum is proportional to its vascularity [10,11]. Many studies have shown that periosteum regenerates bone as well as cartilage from its progenitor cells [11,12].…”

Section: Discussionmentioning

confidence: 99%

“…The osteogenic potential of periosteum is proportional to its vascularity [10,11]. Many studies have shown that periosteum regenerates bone as well as cartilage from its progenitor cells [11,12]. The periosteum consists of two discrete layers: an outer fibrous layer containing fibroblasts, blood vessels, and Sharpey's fibers; and an inner cambium layer containing nerve fibers, capillaries, osteoblasts, and undifferentiated mesenchymal stem cells.…”

Section: Discussionmentioning

confidence: 99%

“…The periosteum consists of two discrete layers: an outer fibrous layer containing fibroblasts, blood vessels, and Sharpey's fibers; and an inner cambium layer containing nerve fibers, capillaries, osteoblasts, and undifferentiated mesenchymal stem cells. The latter are able to differentiate into osteoblasts and chondroblasts as a source of growth factors play an important role in the healing and remodeling process at the outer surface of the cortical bone [11,12].…”

Section: Discussionmentioning

confidence: 99%

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Self-Regeneration of the Mandible Following Hemimandibulectomy for Ameloblastoma: A Case Report and Review of Literature

Ahmad

Omami

2012

J. Maxillofac. Oral Surg.

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Resection of the mandible with immediate or delayed graft reconstruction is widely used in the treatment of ameloblastoma involving a large portion of the mandible. The purpose of reconstruction is mainly to restore the esthetic appearance and mandibular function of the patient. Spontaneous regeneration of the mandible after resection is rarely encountered. This article reports a rare case of spontaneous regeneration of the mandible after hemimandibulectomy for ameloblastoma in a 16-year-old male patient. We discuss the theories pertaining to the mechanism and source of the new bone formation in this case and review the English literature.

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“…Two morphologically distinguishable layers can be found in periosteum; (i) the outer, thicker fibrous layer, and (ii) the layer adjacent to the bone called the cambium layer (Figure 1). The definition "cambium" refers to the cambium layer of the trunk of a tree [3]. The cambium layer in the periosteum contains mesenchymal progenitor cells with osteo-and chondrogenic potential.…”

Section: Periosteum -A Historical Perspectivementioning

confidence: 99%

Cartilage Tissue Engineering; Lessons Learned From Periosteum

Emans¹

2011

J Tissue Sci Eng

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Cartilage, due to its unique physiology (lack of vasculature), can be potentially repaired using tissue engineered in the laboratory, by combining cells and with a supporting scaffold. This requires a marriage between material science, cell biology, and translational medicine, a concept well established as Tissue Engineering.Over the years the in vivo and in vitro chondrogenic potential of periosteum has been recognised by many researchers and as such periosteum is explored both to repair cartilage defects directly by transplanting periosteum into the cartilage defect or by using periosteum as a cell source for cartilage engineering purposes. The initial example hereof is the first generation of Autologous Chondrocyte Transplantation. Graft hypertrophy and ossification remain the primary drawbacks of cartilage repair strategies using engineered cartilage. These drawbacks may (partially) be due to the endochondral ossification process that can take over when cartilage is repaired. In this process chondrogenesis of progenitor cells is followed by hypertrophy of these cells and subsequent ossification. Periosteal progenitor cells go through this process in order to heal bone fractures. This review provides an overview of the role of periosteum in cartilage repair and cartilage tissue engineering and illustrates how periosteum can be used as a model to study the endochondral process. Such studies may provide clues to further optimize cartilage tissue engineering by identifying important factors which are capable of maintaining cells in their chondrogenic phenotype. Finally, the use of periosteum to engineer cartilage in vivo at an extra-articular site is described.

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Localization of chondrocyte precursors in periosteum

Cited by 119 publications

References 38 publications

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Human Periosteum Is a Source of Cells for Orthopaedic Tissue Engineering: A Pilot Study

Self-Regeneration of the Mandible Following Hemimandibulectomy for Ameloblastoma: A Case Report and Review of Literature

Cartilage Tissue Engineering; Lessons Learned From Periosteum

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