Localization of decorin gene expression in normal human breast tissue and in benign and malignant tumors of the human breast

Boström, Pia; Sainio, Annele; Kakko, Tanja; Savontaus, Mikko; Söderström, Mirva; Järveläinen, Hannu

doi:10.1007/s00418-012-1026-0

Cited by 35 publications

(52 citation statements)

References 48 publications

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“…Recently, we have shown that different types of human breast cancer cells totally lack decorin mRNA [19]. We have also shown that the adhesion, proliferation and apoptosis of MCF-7 human breast cancer cells can be influenced by decorin transduction [19].…”

Section: Introductionmentioning

confidence: 98%

“…On the other hand, delivery of decorin gene or protein has been demonstrated to lead to growth retardation of different cancers [16]–[18] through various mechanisms of action such as via binding to growth factor receptors mentioned above and modulating their activity [11]. Recently, we have shown that different types of human breast cancer cells totally lack decorin mRNA [19]. We have also shown that the adhesion, proliferation and apoptosis of MCF-7 human breast cancer cells can be influenced by decorin transduction [19].…”

Section: Introductionmentioning

confidence: 99%

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Lack of Decorin Expression by Human Bladder Cancer Cells Offers New Tools in the Therapy of Urothelial Malignancies

et al. 2013

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Decorin, a multifunctional small leucine-rich extracellular matrix proteoglycan, has been shown to possess potent antitumour activity. However, there is some uncertainty whether different cancer cells express decorin in addition to non-malignant stromal cells. In this study we clarified decorin expression by human bladder cancer cells both in vivo and in vitro. In addition, the effect of adenovirus-mediated decorin expression on human bladder cancer cells in vitro was examined. We first demonstrated using the publicly available GeneSapiens databank that decorin gene expression is present in both normal and malignant human bladder tissues. However, when we applied in situ hybridization with digoxigenin-labeled RNA probes for decorin on human bladder carcinoma tissue samples derived from a large radical cystectomy patient cohort (n = 199), we unambiguously demonstrated that invasive and non-invasive bladder carcinoma cells completely lack decorin mRNA. The cancer cells were also negative for decorin immunoreactivity. Instead, decorin expression was localized solely to original non-malignant stromal areas of bladder tissue. In accordance with the aforementioned results, human bladder cancer cells in vitro were also negative for decorin expression as shown by RT-qPCR analyses. The lack of decorin expression by bladder cancer cells was shown not to be due to the methylation of the proximal promoter region of the decorin gene. When bladder cancer cells were transfected with a decorin adenoviral vector, their proliferation was significantly decreased. In conclusion, we have shown that human bladder cancer cells are totally devoid of decorin expression. We have also shown that adenovirus-mediated decorin gene transduction of human bladder cancer cell lines markedly inhibits their proliferation. Thus, decorin gene delivery offers new potential therapeutic tools in urothelial malignancies.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Lack of Decorin Expression by Human Bladder Cancer Cells Offers New Tools in the Therapy of Urothelial Malignancies

et al. 2013

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“…Extracellular matrix macromolecules are involved in cellular physiologic events and pathological processes (43). Among these, Fmod and Dcn, extracellular matrix proteoglycans, have been designated as potent antitumor molecules and the lack of these proteins is permissive for tumorigenesis (44).…”

Section: Discussionmentioning

confidence: 99%

Recombinant fibromodulin and decorin effects on NF-κB and TGFβ1 in the 4T1 breast cancer cell line

et al. 2017

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Abstract. Constitutive activation of nuclear factor-κB (NF-κB) stimulates cell proliferation and metastasis, and inhibits apoptosis in breast cancer. Transforming growth factor-β (TGF-β) signaling pathway is deregulated in breast cancer progression and metastasis. The aim of the present study was to investigate the inhibitory effects of the two small leucine rich proteoglycans fibromodulin (Fmod) and decorin (Dcn), overexpressed using adenovirus gene transfer, on NF-κB-activity and TGF-β1-expression in the highly metastatic 4T1 breast cancer cell line. The results demonstrate that Fmod and Dcn overexpression is associated with NF-κB and TGF-β1 downregulation, and that Fmod promotes this effect more effectively compared with Dcn.

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“…This protein is found in normal connective tissues, including the bone, where it binds to and cross-links collagen fibrils [14]. Decorin is also found in tumor ECM and is mainly attributed to surrounding, non-malignant stromal cells (tumor cells express little decorin) [15–17]. However, decorin expression is decreased in stromal cells of many tumors compared to normal tissues and inversely correlates with prognosis of breast and lung carcinomas [16, 18, 19].…”

Section: Small Leucine-rich Proteoglycans (Slrps)mentioning

confidence: 99%

Matricellular proteins as regulators of cancer metastasis to bone

Trotter

Yang

2016

Matrix Biology

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Metastasis is the major cause of the death in cancer patients, and a frequent site of metastasis for many cancers is the bone marrow. Therefore, understanding the mechanisms underlying the metastatic process is necessary for future prevention and treatment. The tumor microenvironment is now known to play a role in the metastatic cascade, both at the primary tumor and in metastatic sites, and includes both cellular and non-cellular components. The extracellular matrix (ECM) provides structural support and signaling cues to cells. One particular group of molecules associated with the ECM, known as matricellular proteins, modulate multiple aspects of tumor biology, including growth, migration, invasion, angiogenesis and metastasis. These proteins are also important for normal function in the bone by regulating bone formation and bone resorption. Recent studies have described a link between some of these proteins and metastasis of various tumors to the bone. The aim of this review is to summarize what is currently known about matricellular protein influence on bone metastasis. Particular attention to the contribution of both tumor cells and non-malignant cells in the bone has been given.

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Localization of decorin gene expression in normal human breast tissue and in benign and malignant tumors of the human breast

Cited by 35 publications

References 48 publications

Lack of Decorin Expression by Human Bladder Cancer Cells Offers New Tools in the Therapy of Urothelial Malignancies

Lack of Decorin Expression by Human Bladder Cancer Cells Offers New Tools in the Therapy of Urothelial Malignancies

Recombinant fibromodulin and decorin effects on NF-κB and TGFβ1 in the 4T1 breast cancer cell line

Matricellular proteins as regulators of cancer metastasis to bone

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