Localization of Dengue Virus in Naturally Infected Human Tissues, by Immunohistochemistry and In Situ Hybridization

Jessie, Kala; Fong, Mun Yik; Devi, Shamala; Lam, Sai Kit; Wong, Kum Thong

doi:10.1086/383043

Cited by 519 publications

(494 citation statements)

References 33 publications

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“…Infected dendritic cells mature and migrate to local or regional lymph nodes where they present viral antigens to T cells, initiating the cellular and humoral immune responses. There is also evidence of abundant replication of DENVs in liver parenchymal cells and in macrophages in lymph nodes, liver and spleen, as well as in peripheral blood monocytes 17 . Both in vitro and in vivo, macrophages and monocytes participate in antibody-dependent enhancement (ADE) [18][19][20] .…”

Section: Dengue Virus Pathogenesismentioning

confidence: 99%

Dengue: a continuing global threat

et al. 2010

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Dengue fever and dengue haemorrhagic fever are important arthropod-borne viral diseases. Each year, there are ~50 million dengue infections and ~500,000 individuals are hospitalized with dengue haemorrhagic fever, mainly in Southeast Asia, the Pacific and the Americas. Illness is produced by any of the four dengue virus serotypes. A global strategy aimed at increasing the capacity for surveillance and outbreak response, changing behaviours and reducing the disease burden using integrated vector management in conjunction with early and accurate diagnosis has been advocated. Antiviral drugs and vaccines that are currently under development could also make an important contribution to dengue control in the future.Dengue is the most important arthropod-borne viral infection of humans. Worldwide, an estimated 2.5 billion people are at risk of infection, approximately 975 million of whom live in urban areas in tropical and sub-tropical countries in Southeast Asia, the Pacific and the Americas 1 . Transmission also occurs in Africa and the Eastern Mediterranean, and rural Europe PMC Funders GroupAuthor Manuscript Nat Rev Microbiol. Author manuscript; available in PMC 2015 February 19. Published in final edited form as:Nat Rev Microbiol. 2010 December ; 8(12 0): S7-16. doi:10.1038/nrmicro2460. Europe PMC Funders Author ManuscriptsEurope PMC Funders Author Manuscripts communities are increasingly being affected. It is estimated that more than 50 million infections occur each year, including 500,000 hospitalizations for dengue haemorrhagic fever, mainly among children, with the case fatality rate exceeding 5% in some areas [1][2][3][4] . The geographical areas in which dengue transmission occurs have expanded in recent years (FIG. 1), and all four dengue virus serotypes (DENV-1-4) are now circulating in Asia, Africa and the Americas, a dramatically different scenario from that which prevailed 20 or 30 years ago (FIG. 2). The molecular epidemiology of these serotypes has been studied in an attempt to understand their evolutionary relationships 11 .This Review will provide an update on our understanding of the pathogenesis of this successful pathogen, how we diagnose and control infection and the progress that has been made in vaccine development. Dengue virus pathogenesisDengue viruses belong to the genus flavivirus within the Flaviviridae family. DENV-1-4 evolved in non-human primates from a common ancestor and each entered the urban cycle independently an estimated 500-1,000 years ago 12 . The virion comprises a spherical particle, 40-50 nm in diameter, with a lipopolysaccharide envelope. The positive singlestrand RNA genome (FIG. 3), which is approximately 11 kb in length, has a single open reading frame that encodes three structural proteins -the capsid (C), membrane (M) and envelope (E) glycoproteins -and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5 [18][19][20] . ADE occurs when mononuclear phagocytes are infected through their Fc receptors by immune complexes that form between DE...

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Section: Dengue Virus Pathogenesismentioning

confidence: 99%

Dengue: a continuing global threat

et al. 2010

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“…After this primary viraemia circulating monocytes within the blood and macrophages within the liver, spleen and bone marrow are infected. 125,126 The range of tissues and cell types infected with DEN viruses suggests that the host receptor(s) are broadly distributed. To date, there is evidence for several candidate host receptors, for example mannose binding protein, heparan sulphate, chondroitin sulphate and DC-SIGN.…”

Section: Cellular and Tissue Targets Of Den Virus Infectionmentioning

confidence: 99%

The pathogenesis of dengue

Whitehorn

Simmons

2011

Vaccine

226

185

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“…107(8): 1021-1029, December 2012 Clinical and pathological evidence implicating abnormal cytokine release as the main mediator of disease and an increased risk of severe dengue disease have been described during secondary dengue virus (DENV) infections (Kurane & Ennis 1992). Indeed elevated levels of tumour necrosis factor (TNF)-α have been detected in dengue haemorrhagic fever/dengue shock syndrome (DHF/DSS) patients (Vitarana et al 1991, Hober et al 1993, Nguyen et al 2004) and the production of this cytokine was correlated with increased numbers of macrophages/monocytes (de-Oliveira-Pinto et al 2012), which are potent phagocytic cells and primary targets for DENV infection (Kangwanpong et al 1995, Jessie et al 2004. These cells express all three classes of FCγR making them especially prone to DENV entry in the form of virus-antibody immune complexes ).…”

mentioning

confidence: 99%

Environmental influences on antibody-enhanced dengue disease outcomes

Diniz

Fôro

Turiel³

et al. 2012

Mem. Inst. Oswaldo Cruz

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Because an enriched environment (EE) enhances T-cell activity and T-lymphocytes contribute to immunopathogenesis during heterologous dengue virus (DENV) infections, we hypothesised that an EE increases dengue severity. To compare single serotype (SS) and antibody-enhanced disease (AED) infections regimens, serial intraperitoneal were performed with DENV3 (genotype III) infected brain homogenate or anti-DENV2 hyperimmune serum followed 24 h later by DENV3 (genotype III) infected brain homogenate. Compared AED for which significant differences were detected between the EE and impoverished environmental (IE) groups (Kaplan-Meyer log-rank test, p = 0.0025), no significant differences were detected between the SS experimental groups (Kaplan-Meyer log-rank test, p = 0.089). Survival curves from EE and IE animals infected with the AED regimen were extended after corticoid injection and this effect was greater in the EE than in the IE group (Kaplan-Meyer log-rank test, p = 0.0162). Under the AED regimen the EE group showed more intense clinical signs than the IE group. Dyspnoea, tremor, hunched posture, ruffled fur, immobility, pre-terminal paralysis, shock and death were associated with dominant T-lymphocytic hyperplasia and presence of viral antigens in the liver and lungs. We propose that the increased expansion of these memory T-cells and serotype cross-reactive antibodies facilitates the infection of these cells by DENV and that these events correlate with disease severity in an EE

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Localization of Dengue Virus in Naturally Infected Human Tissues, by Immunohistochemistry and In Situ Hybridization

Cited by 519 publications

References 33 publications

Dengue: a continuing global threat

Dengue: a continuing global threat

The pathogenesis of dengue

Environmental influences on antibody-enhanced dengue disease outcomes

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