Localization of gene expression for phosphatidylinositol transfer protein in the brain of developing and mature rats

Regulation of PI3K signalling by the phosphatidylinositol transfer protein PITPα during axonal extension in hippocampal neurons

“…1A; see Fig. S1 in the supplementary material), supporting previously reported mRNA distributions (Imai et al, 1997;Utsunomiya et al, 1997). PITPβ, by sequence and structure a closely related PITP, is also expressed in the brain, although levels are substantially lower than levels of PITPα ( Fig.…”

“…PITPs are lipid transfer proteins that can bind and transfer PtdIns and PtdCho, thereby regulating PI metabolism and membrane traffic. Detailed information on the tissue distribution of PITPα and PITPβ protein isoforms is not available, although evidence has been presented for an enriched mRNA expression of both isoforms in the brain (Imai et al, 1997;Utsunomiya et al, 1997). Here, we report on the expression of PITPα protein in the nervous system at embryonic and adult stages.…”

Cloning and characterization of a novel variant (mM‐rdgBβ1) of mouse M‐rdgBs, mammalian homologs of Drosophila retinal degeneration B gene proteins, and its mRNA localization in mouse brain in comparison with other M‐rdgBs

“…This finding suggests that mM‐rdgBβ1 is involved in the neural cell proliferation at least during the pre‐natal stages, while mM‐rdgBβ exerts its influence mainly in differentiating and mature neurons during both pre‐ and post‐natal stages. Significantly positive expression signals for mM‐rdgB1 and mM‐rdgB2 are not detected in the ventricular zone, although the signals have been detected there for PITPα and β in a previous study (Utsunomiya et al . 1997).…”

“…With this novel identification of mM‐rdgBβ1 , it is now clear that two isoforms are present in each of the two subfamilies of mM‐rdgBs as well as the family of PITP. When the localization patterns of mRNAs for these six molecules are compared, according to the present study and our previous study (Utsunomiya et al . 1997), several features can be noted in addition to the mutual spatio‐temporal differences between mM‐rdgBβ1 and mM‐rdgBβ described above.…”

“…During the study, a novel variant of mouse (m) M-rdgBb was identified by a cDNA cloning analysis. In the present report, the molecular characteristics of the novel form, termed mM-rdgBb1, and its localization in cultured pheocromocytoma cells (PC12) cells, as well as in in vivo brain, were clarified and compared with those of the three known mM-rdgBs, and with those of the two PITPs previously reported by us (Utsunomiya et al 1997).…”