Localization of myosin-Va in subpopulations of cells in rat endocrine organs

Espíndola, Foued Salmen; Banzi, Silmara R.; Calábria, Luciana Karen; Custódio, Rogério; Oliveira, Rita C. M.; Procópio, Leandro D.; Lima, Andreia Barcelos Passos; Cunha-Júnior, Jair P.; Coelho, Milton V.; Guedes, Iêda Maria Louzada; Pellizzon, Cláudia Helena; Larson, Roy Edward; Espreáfico, Enilza Maria

doi:10.1007/s00441-008-0630-8

Cited by 10 publications

(6 citation statements)

References 66 publications

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“…The coverslips were washed once with PBS for 10 min and then were incubated with blocking solution (PBST 0.1% with 10% NGS and 0.5% BSA) for 30 min in a moist chamber at room temperature. The blocking solution was gently removed and coverslips were incubated with the primary antibodies to the medial tail of myosin‐Va (1:250 – Espindola et al, ); Tuj1 (1:500 – Covance, USA), TrkA (1:200 – Epitomics), GFP (1:1,000 – Abcam) and Myc (1:200 – Invitrogen) overnight. They were washed once with PBS and exposed to the secondary antibody Alexa 488‐conjugated goat anti‐rabbit (1:500, Molecular Probes), Alexa 568‐conjugated goat anti‐mouse (1:500, Molecular Probes) and Alexa 488‐conjugated goat anti‐mouse (1:500, Molecular Probes).…”

Section: Methodsmentioning

confidence: 99%

Role of myosin Va in neuritogenesis of chick dorsal root ganglia nociceptive neurons

Kanno

Espreáfico

Yan

2013

Cell Biology International

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Myosin-Va, widely distributed throughout the developing nervous system, is involved in the transport of vesicles and other intracellular components with its globular tail domain (GTD) implicated in cargo recognition/interaction. Inactivation of myosin-Va in dorsal root ganglia (DRG) neurons of chick embryos, in vitro, decreases the rate of filopodial extension. MYO5A mutant mice have severe neurological defects. We have found that the overexpression of GTD in DRG cultures reduces the number of neurons with long neurites (above fourfold cell body length) and increased the number of neurons with short or no neurites. However, if transfection occurred after the onset of neuritogenesis, this was not seen. In embryo, we characterized the expression pattern of myosin-Va during neuritogenesis of TrkA-positive cells at different stages of chick DRG development. Myosin-Va expression was detected starting from HH25. At this stage, it was present in cells both with and without neurites. The presence of myosin-Va in DRG neurites persisted throughout the last stage analysed (HH34). The data suggest that Myosin Va can participate in embryonic DRG neuritogenesis.

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Section: Methodsmentioning

confidence: 99%

Role of myosin Va in neuritogenesis of chick dorsal root ganglia nociceptive neurons

Kanno

Espreáfico

Yan

2013

Cell Biology International

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“…Rabbit affinity-purified polyclonal antibodies included: antichicken brain myosin-Va (anti-myosin-Va) head domain recombinant protein [29][30][31] and anti-glucose transporter GLUT4 (anti-GLUT4) by Abcam Inc. (Cambridge, USA). Mouse monoclonal antibodies used: anti-calcium/calmodulin-dependent protein kinase IIα (anti-αCaMKII), anti-synaptosomal-associated protein-25 (anti-SNAP-25), and anti-synapsin by BD Transduction (San Jose, USA) and antiβ-actin by Sigma-Aldrich (St. Louis, USA).…”

Section: Antibodiesmentioning

confidence: 99%

Vochysia rufa and glibenclamide promote effects on oxidative damage and in proteins implicated in vesicular trafficking in diabetic rat brain

Costa¹,

Calábria²,

Rezende³

et al. 2022

OAJS

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Many Vochysiaceae species are widely used in folk medicine to treat some diseases. Vochysia rufa, popularly known as “quina-doce”, has been used in folk medicine to treat type 1 and type 2 diabetes mellitus in the state of Minas Gerais, Brazil. Although the antidiabetic and antioxidant effects and phytochemical profile of Vochysia rufa have already been elucidated, further studies are needed on the effects of this treatment in specific tissues, such as the brain. This study investigated the effect of aqueous extract of Vochysia rufa in diabetic rat brains and for this purpose, oxidative stress markers and the expression/localization of proteins implicated in vesicular trafficking were evaluated. Thirty-two rats were randomized into four groups (non-diabetic, diabetic non-treated, diabetic treated for 43 days with glibenclamide - 6 mg/kg or Vochysia rufa - 500 mg/kg). The extract was administered by gavage for 43 days. Analyses were conducted of enzymes concentration and activity in the brain. The protein levels and localization of myosin-Va, CaMKII, synapsin, SNAP-25 and GLUT4 were also analyzed. Vochysia rufa extract decreased superoxide dismutase, glutathione peroxidase, reduced glutathione, total sulfhydryl and lipid peroxidation levels and increased glutathione S-transferase levels. Additionally, Vochysia rufa treatment increased the expression of myosin-Va, CaMKII and also synapsin, which were confirmed by immunolocalization. The treatment with aqueous extract of Vochysia rufa reduces oxidative stress on diabetic rat and protecting the brain from damage caused by hyperglycemia.

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“…In the present study, in addition to the islet dimension and composition, we used antibodies against the myosin Va (MyoVa) motor protein to document their functionality. The myosin motor protein plays a crucial role in maintaining many biological functions, including the maturing, mobilization, maintenance, and exocytosis of the dense core vesicles in humans [19,20]. In our study, we have considered MyoVa out of all the myosin classes, as this protein is highly expressed in the endocrine cell region and is also directly involved in the localization and secretion of the endocrine granules, especially in neurons and neuroendocrine cells [19,20].…”

Section: Introductionmentioning

confidence: 99%

“…The myosin motor protein plays a crucial role in maintaining many biological functions, including the maturing, mobilization, maintenance, and exocytosis of the dense core vesicles in humans [19,20]. In our study, we have considered MyoVa out of all the myosin classes, as this protein is highly expressed in the endocrine cell region and is also directly involved in the localization and secretion of the endocrine granules, especially in neurons and neuroendocrine cells [19,20]. The primary objective of this study is to document the cellular composition (proportion of alpha cells, beta cells, and NAB cells) in islets of various dimensions and regions of the pancreas, along with their secreting capacity, employing the expression of the MyoVa motor protein in the non-diabetic Indian population.…”

Section: Introductionmentioning

confidence: 99%

Islet Dimensions and Its Impact on the Cellular Composition and Insulin-Secreting Capacity: Insights Into the Role of Non-beta Cells

Mishra,

Sahu,

Naik

et al. 2024

Cureus

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Studies have underscored the significance of islet dimensions, encompassing i) the necessity for islets to maintain an optimal diameter to sustain functional activity; ii) larger islets exhibit an intermingled architecture of alpha and beta cells, enhancing functional activity through paracrine effects; iii) nonalpha/beta (NAB) cells play a significant role in regulating beta cells; and iv) there is a preferential loss of larger islets in cases of type 2 diabetes mellitus.To delve deeper into these aspects, the authors documented the cellular composition in islets of various dimensions and regions of the pancreas, along with their secreting capacity, using the expression of the myosin Va motor protein in nine non-diabetic adult human pancreases. The proportion of NAB cells was found to be higher in intermediate islets and significantly lower in smaller and larger islets.By comparing the differences in islet composition, where NAB cells increase from smaller to intermediate islets, leading to a decrease in the proportion of alpha and beta cells, and in larger islets, there is a higher proportion of beta and alpha cells similar to smaller islets, we propose the hypothesis that NAB cells proliferate as islets increase in size. Furthermore, in larger islets, these NAB cells convert into alpha and beta cells, resulting in the scattered, intermingled arrangement observed in larger islets.The higher intensity of myosin Va expression in the islets of the tail region, along with a similar proportion of NAB cells in intermediate islets of the tail region compared to larger islets, leads to decreased inhibitory stimuli to beta cells and an increased insulin-secreting capacity.

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Localization of myosin-Va in subpopulations of cells in rat endocrine organs

Cited by 10 publications

References 66 publications

Role of myosin Va in neuritogenesis of chick dorsal root ganglia nociceptive neurons

Role of myosin Va in neuritogenesis of chick dorsal root ganglia nociceptive neurons

Vochysia rufa and glibenclamide promote effects on oxidative damage and in proteins implicated in vesicular trafficking in diabetic rat brain

Islet Dimensions and Its Impact on the Cellular Composition and Insulin-Secreting Capacity: Insights Into the Role of Non-beta Cells

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